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酶染
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  enzyme staining
     RESULTS: ①Staining feature of motor tract: Under high power lens it was shown that most fibers were moderate intensity positive reaction, enzyme staining limited at neuraxis, no staining at myelin sheath. Under low power lens only a few sparse block-shape enzyme staining regions were shown.
     结果:①运动束染色特点:高倍镜下见多数纤维染色呈现中等强度阳性反应,酶染色局限于轴索,髓鞘不染色,低倍镜下见只有少量稀疏的块状酶染区。
短句来源
     ②Staining feature of sensory tract: Under high power lens enzyme reaction of resoluble myelinated fiber was negative, but in it there was a mass of strong positive block-shape enzyme staining region (nonmyelinated fiber region). Under low power lens the fibers showed confertim clumping shape and evenly distributed in nerve tract.
     ②感觉束染色特点:高倍镜下所能分辨的有髓纤维,酶反应阴性,但是其间有大量强阳性的群块状酶染区(无髓纤维分布区),低倍镜下呈密集的团块状,均匀分布于神经束内。
短句来源
     But the two kinds of nerve fibers could be seen clearly based on their enzyme staining feature.
     而后相互交错,但是神经纤维的两类来源,根据其酶染特征,还是清晰可辩;
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  “酶染”译为未确定词的双语例句
     After producing mouse anti_cellulase BXC60 polyclonal antibody (anti_BXC60), the specificity of Anti_BXC60 was verified by SDS_PAGE, cellulase staining, western blot and ELISA method.
     制备松材线虫特定纤维素酶的多克隆抗体(Anti_BXC60),通过SDS_PAGE、纤维素酶酶染分析、免疫印迹以及ELISA方法考察该抗血清的特异性。
短句来源
     85.71 % 24/28)of the alpha-fetoprotein positive hepatocytes were found in host liver tissues.
     (3)85.71%(24/28)宿主肝细胞AFP免疫酶染阳性,多呈全胞浆型分布,阳性程度多为弱至中等度。
短句来源
     Methods:Thermostable lactase was separated by disc polyacrylamide gel electrophoresis, the lactase was stained with x-gal,and recovered from single electrophoretic strip.
     方法:采用聚丙烯酰胺凝胶盘状电泳分离耐热乳糖酶,用5-溴-4-氯-3-吲哚-β-D半乳糖苷(x-gal)进行酶染显色,切下单一电泳带进行回收;
短句来源
     In order to explore the pathogenesis, virus antigen of EHF in 20 sampls of gastric mucosa were detected by immunohistochemical staining. Results were positive in 17 of 20 samples, suggesting that the lesion of gastric mucosa was directly related to the virus antigen of EHF.
     为研究其发病机制,应用免疫组化酶染法对20例EHF患者的胃粘膜进行EHF病毒抗原定位检测,17/20例获得阳性结果,初步阐明了上消化道出血/胃粘膜损害与EHF病毒抗原的亲嗜及侵袭有关。
短句来源
     Methods Immunoenzymatic staining and triple antigen glass slide were adopted for immunoassay of schistosomiasis, malaria and filariasis, as well as blood test of plasmodia.
     方法 采用免疫酶染技术及三联抗原片 ,对外来流动人员进行血吸虫免疫测定、疟疾血片免疫测定及疟原虫血检和丝虫免疫测定。
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  相似匹配句对
     The Application of the Biological Enzyme in Dyeing and Finishing Process
     生物整生产中的应用
短句来源
     The problem of the indigo-backstaining in treatment with cellulases
     牛仔布洗中的返问题
短句来源
     Enzyme mimics
     模型
短句来源
     Immobilized enzymes
     固相
短句来源
     the dyeing dynamic is first order when m=1 and α exhaustion rate.
     α为上率。
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  enzyme staining
We sought to learn whether the tectal afferents to LPm might themselves be the source of the enzyme staining by examining the effects of collicular lesions on the thalamic staining pattern.
      
Vessel ingrowth could be observed with enzyme staining for AP, ?-Gluc, NAG, ALP, APA and APM.
      
The use of a fixative solvent mixture prior to the enzyme staining reaction appeared to labilize lysosomal membranes, to improve fixation and to eliminate "fiber" artefacts.
      
The dual localization of certain hydrolases in lysosomes and in endoplasmic reticulum as studied in enzyme staining reactions is now supported by cytobiochemical studies on mouse liver and kidney β-glucuronidase and acid phosphatase.
      
The cells are cultured on a polyester foil and incubated for the enzyme staining reaction on the membrane.
      
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Acid α-Naphthyl acetate esterase (ANAE) method was used to paraffin section to study the ANAE activity and distribution in the developing bursa of Fabricius of White Peking Duck in embryonic and duckling stages, The results indicate that 1. "Tlike" ANAE positive lymphocytes first appear in the follicles of 28-day embryos and its number increases slightly after hatching. 2. In the development of the lympho-epi-thelium follicle, the surface and basal layer of mucosal epithelium are tending to different directions...

Acid α-Naphthyl acetate esterase (ANAE) method was used to paraffin section to study the ANAE activity and distribution in the developing bursa of Fabricius of White Peking Duck in embryonic and duckling stages, The results indicate that 1. "Tlike" ANAE positive lymphocytes first appear in the follicles of 28-day embryos and its number increases slightly after hatching. 2. In the development of the lympho-epi-thelium follicle, the surface and basal layer of mucosal epithelium are tending to different directions of differentiation. Comparing the results with that of ALP, it is interesting to note that the follicle-associated epithelium (FAE) and the reticulo-epi-thelial cells in the center of the follicular medulla are "M-like" ANAE positive in certain stages, the basal layer of the mucosal epithlium, the marginal layer of the follicular medulla and the reticulo-epithelial cells near the marginal layer are ALP positive, but ANAE negative. The above results were discussed in relation to the mi-croenviroment for B lymphocyte development.

石蜡切片α-乙酸萘酯酶(ANAE)染色法用于研究北京鸭胚及胚后早期腔上囊发育的不同阶段。观察了淋巴细胞的ANAE反应特点,并将滤泡上皮和网状上皮成分的ANAE反应与对应的碱性磷酸酶(ALP)染色对比,结果表明:T型ANAE阳性淋巴细胞最初出现在胚胎28天。孵出后,数量略有增加。淋巴滤泡的联滤泡上皮,联滤泡上皮下方以及髓质中央的网状上皮细胞呈M型ANAE阳性,滤泡髓质边缘层及近边缘层的网状上皮细胞ALP阳性,ANAE阴性。值得了注意的是在胚胎发育中,酶染特性不同的两种上皮细胞分别来自于粘膜上皮的表层和基层。讨论了B淋巴细胞在腔上囊中发育的微环境。

Coagglutination (COAG) test and HRP-SPA staining were employedin the experiment. The results obtained are summarized as follows: It was foundthat pH value of PBS in which the reagent suspended is a critical factor. The re-agent prepared with pH 6.0 PBS shows the highest sensitivity for salmonella de-tection, approximately 1. 25~2. 5×10~7 salmonella cells per ml could te detected.HRP-SPA staining has a higher sensitivity, too. Results obtained by using referen-ce strain of salmonella showed that approximately...

Coagglutination (COAG) test and HRP-SPA staining were employedin the experiment. The results obtained are summarized as follows: It was foundthat pH value of PBS in which the reagent suspended is a critical factor. The re-agent prepared with pH 6.0 PBS shows the highest sensitivity for salmonella de-tection, approximately 1. 25~2. 5×10~7 salmonella cells per ml could te detected.HRP-SPA staining has a higher sensitivity, too. Results obtained by using referen-ce strain of salmonella showed that approximately 1. 5625~3. 125×10~6 salmonellacells per ml gave a positive reaction. No cross reactions occurred with unrelated,except for staphylococcus aureus, but it could be eliminated by the morphologicalfeature. Of 236 samples (bone meal 155, fish meal 73, dried blood 8 ) tested, 45(19. 1% )were positive by HRP-SPA staining, 43(18. 2%) were positive by COAGtest, 41(17.4% ) were positive by the conventional cultural technique (CCT). Thepercentage agreement when comparing the COAG test and HRP-SPA staining tothe CCT was 95. 3% and 91.1% respectively. Salmonellae in contaminated feeds could be detected by COAG test and HRP-SPA staining within 18 h. Fortyone salmonella were isolated from 236 samples. The rate of salmonella contamina-tion varied according to the nature of the feed samples tested. sixteen(10.3%)ofbone meal, 23(31. 5%) of fish meal, 2(25%) of dried blood contained salmonella.

本研究确定了适用于动物性饲料中沙门氏菌检验的增菌程序、协同凝集试验(COAG)及HRP—SPA染色法(酶染法)的最适实验条件。以这种条件进行增菌,并以COAG及酶染法进行检查,具有快度、敏感、特异性强的优点。应用COAG、酶染法及沙门氏菌常规分离鉴定法,对236份动物性饲料标本进行了检测。结果两种快速法与常规法有较好的符合率,酶染法在检出阳性标本上优于常规法.且可在16~18h内报告结果,比常规法的时间明显缩短。因此认为,COAG和酶染法适用于动物性饲料中沙门氏菌的快速检测。动物性饲料的沙门氏菌污染,依饲料的种类不同而异,其中鱼粉为31.5%、血粉25%骨粉10.3%,平均为17.4%,而且存在着多种血清型的沙门氏菌,其中以鼠伤寒沙门氏菌占优势。因此认为动物性饲料中的沙门氏菌污染在人畜沙门氏菌的流行病学上具有重要的作用。

The immunoporoxidase staining technics (IPST) was used in 37 patients with epidemic hemorrhagic fever (EHF) for detecting the specific IgM antibodies to EHF and compared with the method of IFA-IgG. The sera obtained at the early stage of the disease were ana- lyzed. In two cases, the results were negative by using the method of IPA-IgM antibodies. In 35 cases, the results were significantly higher than those by means of the method of IFA-IgG. But, the sera obtained at the late stage showed no difference between...

The immunoporoxidase staining technics (IPST) was used in 37 patients with epidemic hemorrhagic fever (EHF) for detecting the specific IgM antibodies to EHF and compared with the method of IFA-IgG. The sera obtained at the early stage of the disease were ana- lyzed. In two cases, the results were negative by using the method of IPA-IgM antibodies. In 35 cases, the results were significantly higher than those by means of the method of IFA-IgG. But, the sera obtained at the late stage showed no difference between the two methods. The inhibition test and 2-ME tolerance tcst showed that the antibodics detected by the IPST technics were specific IgM ones, which could be found in the patient's serum on the second day of onset. The results showed that the IPA-IgM method is sensitive and highspecific and the results can be read under the light microscope. Thus, this method is convenient for the country doctors to use.

本实验重点以免疫酶染检测了37例临床确诊EHF患者双份血清中的IgM抗体并与IFA—IgG抗体法进行了对比,所查37例EHF患者中2例早晚期血清免疫酶染IgM及FA—IgG抗体均为阳性,其余35例早期血清IgM抗体阳性检出率明显高于IFA—IgG法;晚期血清二种方法阳性检出率及阳性检出符合率为100%。阻断试验及2—ME耐性试验显示免疫酶染法检出的抗体系特异性IgM;该抗体可在发病第二日出现,整个病程均能检出。实验结果表明,免疫酶染IgM抗体技术敏感性高,特异性强,仅需要光学显微镜,观察清晰,便于基层人员使用。

 
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