MAO A and B genes are located on the X chromosome (Xp11 23) and consist of 15 exons with identical intron exon organization, which suggests that they are derived from the same ancestral gene.
Methods: Using the blast method combining with the common GU-AG splicing method, the exon-intron boundary of c7orf2/lmbr1 gene in human and mice was analyzed.
Methods Genetic DNA was prepared from peripheral blood leukocytes using DNA Isolation Kits for Mammalian Blood. The complete coding region and exon-intron splice sites of CNGA1 gene were amplified with polymerase chain reaction (PCR).
The results show that the genenom include 4 extrons and 3 introns, and the sequence of the extrons was the same to the cDNA of the Anpl-IL-8. The phylogenetic tree of IL-8 indicates the evolutionary pathways to other animals.
WT5”BZ]To investigate the structure of the genomic DNA sequence for ALR,specific PCR method was used to sub clone target fragment from the Wistar rat genome The whole length is 1508bp,including 3 exons and 2 introns The exons are 18bp,197bp and 163bp,each one encode 6aa,64aa and 55aa respectively The lengths of the exon are as long as that of human and mouse [WT5”HZ]
The human PDE6A gene consists of 22 exons spanning about 60 kb with the intron/exon junctions highly conserved in comparison to the mouse and human PDE6B genes.
In nuclear mRNA genes, exon/intron junctions (both exon/intron and intron/exon junctions in this paper) possess the specific duplex pattern with the corresponding ends (3' to 3', 5' to 5') of exons and introns more or less identical.
During the identification of the intron/exon structure of the gene in a normal healthy male subject with a BMI of 23.5 kg/m2, a nucleotide substitution replacing a glycine with a serine was identified at codon 84.
The porcine class II region appears very similar to that of man in number and nature of the class II genes identified and in the intron/exon organization of corresponding genes.
The exon-intron structure of these genes has been analyzed in zebrafish Danio rerio, loach Misgurnus fossilis, fugu Takifugu rubripes, and Nile puffer Tetraodon fahaka.
This is supported by high sequence similarity between mlc1 and mlc3 as well as by the exon-intron structure of these genes and their localization on different chromosomes.