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  urine samples
     Results Expression of Survivin was found in all 53 cases of bladder transitional epidermal cancer by nested RT-PCR,the sensitivity and specificity of expression of Survivin in all urine samples being100%,and 90% respectively.
     结果用巢式RT-PCR检测53例膀胱移行上皮癌患者尿及癌组织中Survivin均有表达,所有样本尿中Survivin的表达敏感性为100%,特异性为90%。
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     Whether the VB 2 intake is sufficient or not, VB 2/creatine ratios of morning, random urine samples showed no consistent correlation with VB 2/creatine and VB 2 of 24-hours sample. VB 2/creatine was also inconsistent in different urine samples by individual calculation.
     还发现无论核黄素(VB_2)摄入充足与否,空腹尿、任意尿中VB_2/肌酐与24h尿中VB_2量及其VB_2/肌酐之相关结果也不一致,在同一个体,各不同尿样的VB_2/肌酐也不稳定。
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     The average relative standard deviation for the runs carried out on the same day was approximately 2.9% at the range of 50~200μmol/L of CEMA, and the mean analytical recovery from spiked urine samples was 94%.
     方法检测限为3μmol/L,尿中CEMA浓度为50、200μmol/L时批内平均相对标准偏差为2.9%,尿加标平均回收率为94%。
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     The linear relationship b etween the initial reaction rate and the hemoglobin concentration was in the range of 1×10-9~8×10-8mol/L. The detection limit was 3.4×10-10mol/L. The method was used to determine hemoglobin in the urine samples with satisfactory result.
     在1.5×10-3mol/L SDS存在下,测定Hb的线性范围1×10-9~8×10-8mol/L,检出限为3.4×10-10mol/L,用于尿中Hb的测定,结果满意。
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     Methods:The gB genotypes in the urine samples from 88 newborns with HCMV infection were determined by using nested polymerase chain reaction(nested PCR) and restriction fragment length polymorphism(RFLP). The relationship between the gB genotypes and clinical symptoms were analyzed.
     方法:采用巢式聚合酶链反应(nested PCR),限制性片段长度多态性(RFLP)分析技术等测定88例感染患儿尿中HCMV gB基因型,并分析gB基因型与不同临床症状的关系。
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  “尿中”译为未确定词的双语例句
     A Method for the Separation and Determination of Pregnanediol in Women's Urine
     妇女尿中孕二醇的分离及其含量的比色测定
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     OESTROGENS IN THE URINE OF THE PREGNANT SOW
     孕猪妊娠期间尿中的雌性激素
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     Determination of Microamount of Tin in Urine
     尿中微量锡的测定
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     GAS CHROMATOGRAPHIC DETERMINATION OF THREE ESTROGENS IN NONPREGNANT URINE
     非孕尿中三种雌激素的气相层析测定法
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     Determination of thorium in human urine by co-precipitation method with bismuth phosphate
     磷酸铋共沉淀法测定人尿中的钍
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  相似匹配句对
     Determination of Cretinine in Urine by Flow Injection Analysis
     尿肌酐的流动注射分析
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     d.
     d
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     DETERMINATION OF ~(32)P IN URINE
     尿~(32)P的测定
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  urine samples
The satisfactory results on spiked urine samples are obtained, when the component number was chosen to 3 (N = 3) for both the methods.
      
The proposed method was applied to the determination of OF, NF, GF, and LF in both commercially available drugs and spiked human urine samples.
      
Study of a fluorescence quenching mechanism of enoxacin and its determination in human serum and urine samples
      
The method is simple, rapid, and can be used for the determination of enoxacin in human serum and urine samples with satisfactory results.
      
The stability of zopiclone and its metabolites upon time was studied by analyzing urine samples from patients receiving therapeutic doses of this substance stored for 1, 3, and 6 months.
      
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A method for the paper chromatography of aureomycin is here described. Out of 43 kinds of developing solvents studied, four have been proved satisfactory. They are: (1) n-butanol saturated with M/10 citrate buffer of pH 3.9, (2) 1% aqueous solution of boric acid, (3) M/10 citrate buffer of pH 3.9 saturated with n-butanol, and (4) n-butanol-acetic acid-water (5∶1∶4). The upward R_F values for the above four solvent systems are 0.40 ± 0.02, 0.83 ± 0.02, 0 and 1 respectively when Whatman No. 1 filter paper is...

A method for the paper chromatography of aureomycin is here described. Out of 43 kinds of developing solvents studied, four have been proved satisfactory. They are: (1) n-butanol saturated with M/10 citrate buffer of pH 3.9, (2) 1% aqueous solution of boric acid, (3) M/10 citrate buffer of pH 3.9 saturated with n-butanol, and (4) n-butanol-acetic acid-water (5∶1∶4). The upward R_F values for the above four solvent systems are 0.40 ± 0.02, 0.83 ± 0.02, 0 and 1 respectively when Whatman No. 1 filter paper is used. For general purpose the first solvent is preferred. With this solvent, the R_F values for terramycin hydrochloride, streptomycin hydrochloride-calcium chloride complex and chloromycetin are 0.32±0.03, 0 and 1 respectively. Chromatograms containing aureomycin may be easily recognized with naked eyes if the amount of the antibiotic present is not less than 5 μg/cm~2, or by fuming with HC1- vapour to give an orange stain of anhydroaureomycin hydrochloride if not less than 1.6 μg/cm~2, or by agar-plate method if not less than 0.3 μg/cm~2, or by fluorescence method if not less than 0.09 μg/cm~2. Under an UV-lamp, aureomycin hydrochloride shows a bright lemon-yellow fluorescence; terramycin, dirty yellow; and anhydroaureomycin hydro- chloride-boric acid complex, dull brown. The fluorescing chromatograms can be photo- graphed. The present method can be directly employed for qualitative as well as rough quantitative determination of the aureomycin in the beer of Streptomyces aureofaciens. It may serve as a useful aid in antibiotic screening. By means of mixing chromatography, aureomycin may be detected in the human urine collected after oral administrations. Using 1% boric acid as the developing solvent, anhydroaureomycin hydrochloride can be successfully separated from aureomycin hydrochloride by chromatography. In fact, the existence of a trace of the anhydro-compound has been detected in some crude aureomycin preparations.

本報告提供了一個金黴素的紙上層析方法。在28°用Whatman 1號濾紙研究了43種紙上層析用的顯層溶劑。用其中的四種顯層溶劑,M/10檸檬酸鹽pH 3.9緩衝液飽和的丁醇(I),1%硼酸水溶液(II),丁醇飽和的M/10檸檬酸鹽pH3.9緩衝液(III),和丁醇-醋酸-水(5∶1∶4)(IV)顯層,都可以得到較滿意的色層。它們的比移分別是0.40±0.02,0.83±0.02,0.87±0.01和0.88。這四種中尤以顯層溶劑(I)的結果最佳。用顯層溶劑(I),在同一條件下的鹽酸地黴素、鹽酸鏈黴素氯化鈣複鹽和氯黴素的比移,分別是0.32±0.03,0和1。色層辨認的方法,在超過5微克/厘米~2以上可用肉眼;1.6微克/厘米~2以上可用氯化氫氣體顯色;在0.3微克/厘米~2以上可用瓊脂平板培養基制菌法;在0.09微克/厘米~2以上可用螢光法。色層螢光可以直接攝照。 應用本法可以直接鑑定金黴菌發酵液中的金黴素,並可以初步估計含量。用混合層析法也可以鑑定尿中的金黴素。 應用1%硼酸水溶液作顯層溶劑,可以鑑別鹽酸金黴素和鹽酸脫水金黴素,用本法曾鑑定了幾批鹽酸金黴素粗製品中有微量脫水化合物的存在。

In a previous study, we have compared the physiological disposition of two oral diuretics, chlorothiazidè (CT) and hydrochlorothiazide (HCT). In the present paper, the distribution, absorption and excretion of 5-chloro-2,4-disulfonamidoaniline (DSA), its possible metabolic fate, as well as its diuretic action in rats, are reported.

本研究系比较CT与其水解产物——DSA对大鼠的利尿作用并探讨DSA在大鼠体内的转化、吸收、分布和排泄。大鼠口服20及100毫克/公斤时DSA的利尿及排氯作用与CT相同,口服CT或DSA 4毫克/公斤后,则作用均不明显。水解前后比色或纸层分析都证明服药后的大鼠尿中没有DSA的乙酰化产物或其他代谢产物。口服DSA 20毫克/公斤后,药物被迅速吸收,1、3、9小时的胃腸道含药量分别为口服剂量的55、20及12%。大鼠口服DSA 4—100毫克/公斤后,3小时内自尿排出剂量的30%左右,口服CT4毫克/公斤后同时期内排出剂量的34%,但加大剂量到20及100毫克/公斤时,仅排出剂量的17—18%。反之,静脉注射20毫克/公斤后,CT自尿排出比DSA迅速。自胆汁排出的药量少于口服剂量的1%。大鼠口服DSA 20毫克/公斤后1小时,药物在肾脏分布最多,肝次之。但总的说来,本药在各脏的浓度较趋平均。

It is well known that β-diethylaminoethyl diphenylpropylacetate HCl(SKF 525A) is a potent inhibitor of hepatic microsomal drug-metabolizing enzymes. However, our present study indicates that the effect of SKF 525A on these enzyme systems is actually biphasic. Sodium pentobarbital sleeping-time was used as an indirect criterion of the activity of the drug-transforming enzymes in mice and rats. The rate of disappearance of pentobarbital from whole mouse was determined to assess the effect of SKF 525A on the in...

It is well known that β-diethylaminoethyl diphenylpropylacetate HCl(SKF 525A) is a potent inhibitor of hepatic microsomal drug-metabolizing enzymes. However, our present study indicates that the effect of SKF 525A on these enzyme systems is actually biphasic. Sodium pentobarbital sleeping-time was used as an indirect criterion of the activity of the drug-transforming enzymes in mice and rats. The rate of disappearance of pentobarbital from whole mouse was determined to assess the effect of SKF 525A on the in vivo metabolism of the hypnotic. The amount of pentobarbital metabolized after incubating with rat liver slices was also determined. Goldbaum's method was used for the assay. It was found that either prolongation or shortening of pentobarbital sleeping-time could occur depending upon the length of the time interval between the administrations of SKF 525A(40-80 mg/kg, ip.) and pentobarbital(60 mg/kg, ip.). When pentobarbital was administered to mice within 12 hours after SKF 525A, prolongation of sleeping time was observed. On the other hand, if the mice were treated with SKF 525A 48 hours beforehand, a shortening of sleeping time was noted. Similar results were obtained for both rats and mice receiving daily injections of SKF 525A(10-40 mg/kg/day) for 3-10 days. Further studies showed that the decrease in pentobarbital sleeping time was associated with an increase in the rate of metabolism of the hypnotic. In mice receiving SKF 525A 48 hours prior to injection of pentobarbital, the whole body concentrations of the hypnotic were found to be significantly lower than those of control mice. Furthermore, liver slices from rats treated with a dose of SKF 525A 48 hours previously metabolized more pentobarbital than slices from control animals. However, the minimal body pentobarbital levels required to maintain the mice asleep seemed to be similar in both the experimental and the control groups. This fact indicates that the change in sleeping time cannot be attributed to changes in sensitivity of the central nervous system to pentobarbital. With diethylbarbital(a drug which is not metabolized in vivo), neither sleeping time nor the rate of disappearance of the drug from whole mouse was changed by SKF 525A pretreatment. The ascorbic acid contents of rat urine and mouse liver were also determined by the method of Roe and Kuther. It was found that pretreatment of animals with SKF 525A 12-24 hours before sacrifice led to elevated excretion and higher hepatic levels of ascorbic acid. This effect of SKF 525A disappeared 48 hours after injection. These results show that the enhancing effect of SKF 525A on ascorbic acid metabolism occurs before the stimulatory effect on liver drug-metabolizing enzymes.

已知二苯基丙基乙酸β-二乙基氨基乙酯(SKF 525A)为肝微粒体药物轉化酶的抑制剂。我們以戊巴此妥鈉睡眠时間为指标,发現SKF 525A的作用是双相的。小鼠注射一剂SKF 525A(40—80毫克/公斤)后0—12小时为莉酶受抑制期,表現为戊巴比妥鈉睡眠时間明显延长;但注射后48小时,小鼠戊巴比妥鈉睡眠时間不仅不延长,反而縮短。SKF 525A的双相效应在雌雄小鼠均能看到。小鼠及大鼠經連續多次注射SKF 525A后48小时,同样也出現第二相效应。下述进一步的实驗表明第二相效应是由于肝脏药物轉化酶活性加強的結果:(1)48小时前接受过一剂SKF 525A的小鼠,戊巴比妥鈉自体內消失的速率此正常动物者明显加快。(2)不論48小时前是否接受过SKF 525A,小鼠戊巴比妥鈉睡眠刚醒时,体內催眠药含量无显著差別。(3)48小时前曾經注射SKF 525A的大鼠肝切片轉化戊巴比妥鈉的速率比正常动物肝切片者快。(4)48小时前注射SKF525A并不改变二乙基巴比妥鈉(一个在体內不經轉化的莉物)引起的小鼠睡眠时間及其自体內消失的速度。 給小鼠(或大鼠)注射相当剂量的SKF 525A后12—24小时,肝脏(及尿)中的維生素C...

已知二苯基丙基乙酸β-二乙基氨基乙酯(SKF 525A)为肝微粒体药物轉化酶的抑制剂。我們以戊巴此妥鈉睡眠时間为指标,发現SKF 525A的作用是双相的。小鼠注射一剂SKF 525A(40—80毫克/公斤)后0—12小时为莉酶受抑制期,表現为戊巴比妥鈉睡眠时間明显延长;但注射后48小时,小鼠戊巴比妥鈉睡眠时間不仅不延长,反而縮短。SKF 525A的双相效应在雌雄小鼠均能看到。小鼠及大鼠經連續多次注射SKF 525A后48小时,同样也出現第二相效应。下述进一步的实驗表明第二相效应是由于肝脏药物轉化酶活性加強的結果:(1)48小时前接受过一剂SKF 525A的小鼠,戊巴比妥鈉自体內消失的速率此正常动物者明显加快。(2)不論48小时前是否接受过SKF 525A,小鼠戊巴比妥鈉睡眠刚醒时,体內催眠药含量无显著差別。(3)48小时前曾經注射SKF 525A的大鼠肝切片轉化戊巴比妥鈉的速率比正常动物肝切片者快。(4)48小时前注射SKF525A并不改变二乙基巴比妥鈉(一个在体內不經轉化的莉物)引起的小鼠睡眠时間及其自体內消失的速度。 給小鼠(或大鼠)注射相当剂量的SKF 525A后12—24小时,肝脏(及尿)中的維生素C含量比对照动物者显著增加。但在給药后48小时,此作用即已消失。可見SKF525A对动物体內維生素C合成的促进作用出現在对肝脏莉物轉化酶的刺激作用之前。

 
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