脾毒 spleen virus(6)   spleen virus      Sequencing analysis of E2 gene of muti-passsaging spleen virus and cytopathogenic clsssical swine fever virus      猪瘟病毒石门株动物传代脾毒与组织培养细胞毒E2基因序列的分析 短句来源      The results showed that the nucleotide homology between spleen virus and Shimen was 99.4%,the aminoacid homology 99.0%,the nucleotide homology between cultured cell virus and Shimen 98.3%,the aminoacid homology 96.7%.      结果发现,脾毒和标准石门株核苷酸序列的同源性为99.4%,氨基酸序列的同源性为99.0%; 细胞毒与标准石门株核苷酸序列的同源性为98.3%,氨基酸序列的同源性为96.7%。 短句来源      (2) Swine umbilicus veins was infected by spleen virus and induced pathological changes. The E2 gene of cell virus were cloned and sequenced.      (2)用猪瘟脾毒接种猪血管内皮细胞,对引起细胞病变的细胞毒E2 基因进行克隆测序,比较分析细胞毒E2 基因与标准石门株的核苷酸和氨基酸序列的同源性。 短句来源      The results showed that the nucleotide homologybetween spleen virus and Shimen was 99.4 %,theaminoacid homology was 99.0 %,the nucleotide homology between cultured cell virus and Shimen was 98.3 %,theaminoacid homology was 96.7 %,so we educed that after poly-passaging and Cytopathogenic effect,E2 genes of CSFVwere geneticallystable.      结果发现,脾毒和标准石门株的核苷酸序列同源性为99.4%,氨基酸序列同源性为99.0%,细胞毒标准石门株的核苷酸序列同源性为98.3%,氨基酸同源性96.7%。 由此说明猪瘟病毒经猪多次传代和致细胞病变后均保持遗传的稳定性。 短句来源      The results showed that the nucleotide homology between spleen virus and Shimen was 99.4%,the aminoacid homology was 99.0%. It was showed epitopes of all strains were not variable obviously by analysising the variation of some main amino acid residues substitutions of E2 gene main antigenic domains.      结果发现, 脾毒和标准石门株的核苷酸序列同源性为99.4%,氨基酸序列同源性为99.0%,通过对主要抗原区域氨基酸位点变异进行分析,发现E2 基因抗原决定簇未发生明显的变异。 短句来源 更多          “脾毒”译为未确定词的双语例句      Sequence Analysis of NS2-3 Gene of Hog Cholera Virus Lapinized Chinese Strain      中国猪瘟兔化弱毒兔脾毒NS2-3基因的序列分析 短句来源      3. Cells inoculated by shimen virulent strain and C strain of CSFV and control cells(notinoculated)at 24h,56h were observesed by transmission electron microscopy after fixationwith methyl aldehyde respectively.      3. 取接种 CSFV 石门株脾毒、C 株后 24h 和 56h 的血管内皮细胞和对照血管内皮细胞，经甲醛固定后进行透射电镜观察。 短句来源      The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection      猪瘟病毒C株兔脾毒在SK6细胞中的增殖培养及其鉴定 短句来源      The minimum immunity was 1:10 dilution of qlood in doses of 1ml. 1:100,000 dilution of lymph node and spleen in doses of Ⅰ ml.      并有100％的免疫力,其最小免疫量为原血10倍1毫升,淋脾毒100,000倍1毫升。 短句来源      2. The 2~3th passage endothelial cells were inoculated by shimen virulent strain settingas treatment group;      2. 取 2～3 代生长良好的猪血管内皮细胞，待长至 80%单层时接种 CSFV 石门株脾毒，加维持液（含 2%无牛病毒性腹泻病毒抗体的胎牛血清）培养。 短句来源 更多          相似匹配句对      Splenic Abscess      脾脓肿 短句来源      Hamartoma of the Spleen      脾错构瘤 短句来源      A Poison Tree      毒树 短句来源      Cytochalasin B      细胞毒B 短句来源      The key pathogenesis is accumulation of heat toxin and damage to the liver and spleen.      病机关键为热毒瘀结 ,肝脾损伤。 短句来源 查询“脾毒”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   spleen virus Cold or isolation stress increased blood brain and spleen virus levels as early as 2 days after inoculation. 例句来源                Summary 1.The cell-cultured lapinized strain of swine fever virus was sucessfully propagated on PK primary cell monolayer in rolling bottles, LHHank's solution containing horse or calve serum was used as the medium and no antibiotics were added. The inoculum was a 0.3% of 1:10 spleen suspension of infected rabbit or a 2—4% cell cultured virus. After cultivation at 36℃ the media were havested and changed every 4 days. The titre of havested virus in every run were 2x10~4-5x10~4 in terms of rabbit infective t...             用转瓶培养猪肾细胞生产猪瘟细胞苗,本试验证明①用小黄牛血清或马血清或犊牛血清均可制出较高毒价的猪瘟细胞毒,②营养液中加青、链霉索各90—100μ/ml,接毒后维持液不用抗菌素,可以控制污染,並有利于毒的复制,③按营养液量接种猪瘟兔化毒脾毒0.3％(克)或2—4％细胞培养毒,均可制出毒价5×10~(-4)的细胞苗,但必须保持收液pH在6.8—7.0左右,④残存抗菌素在0.5μ/ml以下的细胞毒,配三联苗后对猪丹毒及猪肺疫菌影响不显著,⑤已连续制三联苗70余批,三种成份分别检验均达到规定标准,田间试使已接种猪1000万头以上,安全有效。 文摘来源          This paper related to a special nerve symtom which was occured from 1950-1951 after the yaks were immune to lapinized rinderpest virus, when yak rinderpest greatly prevailed throughout Qinghai province. When the prevention could not prcceed and a kind of vaccine is urgent in need, a series of scientific research were started. It turned out that when the yaks were inoculated in laboratory with lapinized virus, 16.1% yaks had nerve symtom, the reaction of immunity was also serious, though 10,000 times diluted...             本文叙述了青海省牛痘大流行时,1950—1951年用兔化毒免疫牦牛后,发生一种特异的神经症状。在防疫陷于停顿急待一种疫苗作预防时,开展了一系列研究,试验结果:得出兔化毒在实验室接牦牛后,有61.1％发生了神经症状,免疫反应也较严重。兔化毒虽经稀释10,000倍,仍有1/2发生神经症状。山羊化兔毒接种牦牛后,有17.86％严重反应,虽经稀释至10,000倍,尚有1/2发生严重反应。用不同接种方法;口服有60％、皮内注射有13.33％发生严重反应,甚至死亡。点眼无严重反应,而只获得70％免疫力。山羊化兔毒回归家兔9代接种牦牛,有20％的严重反应,证明兔化毒与山羊化兔毒单独使用,均不能作为牦牛免疫之用。山羊化兔毒与少量抗牛瘟血清共同注射,无严重反应,32—42天后攻毒,有坚强的免疫力,可在疫区使用。绵羊化兔毒能用藏系绵羊继代,感染率可达95.2％。继代羊原血与100倍淋脾毒2毫升肌注牦牛,无严重反应,除体温反应与短期内泌乳量有所减少外,无明显的临床症状;并有100％的免疫力,其最小免疫量为原血10倍1毫升,淋脾毒100,000倍1毫升。免疫期已测至5年零32天,因免疫牛已尽而停顿,估计免疫力... 文摘来源          The envelope glycoprotein E0 gene of hog cholera virus (HCV) strain C was amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested PCR. The PCR product was cloned into pGEM T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device. Based on the incorporation of fluorescence labelled dideoxynuclotide teminators, the sequence was compared with HCV strain C sequenced by Moormann et al . The result showed that their homologies o...             采用异硫氰酸胍一步法从４８０代猪瘟病毒兔化弱毒株（ＨＣＬＶ）脾毒中提取总ＲＮＡ，以该ＲＮＡ为模板，进行反转录，然后采用套式ＰＣＲ扩增出ＨＣＬＶ的囊膜糖蛋白Ｅ０基因，琼脂糖凝胶电泳表明其大小与预计相符。将扩增出的Ｅ０基因克隆到ｐＧＥＭＴ载体中，用自动序列分析仪对其进行序列测定。将测得的序列及推导的氨基酸序列与国外测得的Ｃ株相应序列进行比较，结果发现，它们之间核苷酸序列同源性为９９．０８％，氨基酸序列同源性为９８．４２％。 文摘来源   << 更多相关文摘         相关查询: 脾 毒 脾— 毒蛛 两栖动物毒 脾肾的 脾病 脾梗塞 脾片 癌毒 毒率 出血毒  对查询结果不满意

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