Among them, fractions with molecular weight ranges lower than 5kD(SISE p1) and from 5kD to 10kD(SISE p2) had relatively higher immunosuppressive activity, which could inhibit phytohemagglutinin(PHA) induced normal human peripheral blood lymphocyte(PBL) transformation by 59.8% and 61.7% respectively at a concentration of 6.8 μg/mL in vitro .
METHODS: Monocytes were isolated from normal human peripheral blood cells by density gradient centrifugation and the plastic-adherent cells were cultured with the cytokines ( rhGM-CSF , rhIL-4, rhTNF-α).
Objective:To evaluate the effects of lipopolysaccharide(LPS) and Staphylococcus aureus(SAC) on IL-23 and IL-12 subunits expression in normal human peripheral blood mononuclear cells (PBMC) ,and explored the regulation mechanisms.
AIM: To study the effects of morphine on tumor necrosis factor α (TNF α) and interleukin 6 (IL 6) production in human whole blood and the possible mechanism.
Methods Peripheral blood lymphocytes were treated with 10 -5 mol/L Sudan I for 8 h. The total RNA from cells of the subjects of the treated group and the control group were isolated, then they were reversely transcribed and labeled with fluorescence dyes, and finally hybridized with human whole genome microarray (about 35,000 spots).
The result shows that Dangui Yinzi within 10~(-1)~10~(-7)mg/ml can uniquely or together with PHA promote the monocyte hyperplasia in human being peripheral blood.
MethodTake normal persons as control,combine CD_3,CD_4,CD_8 with human being peripheral blood monocytes,analyse with flow cell method the cells of CD~+_3,CD~+_4,CD~+_8 in monocyte and CD~+_4/CD~+_8.Compare and analyse T cell subset in 3 groups before and after treatment.
A synthetic peptide was shown to bind to TACE and to inhibit TNF-α release from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) by up to 60.3%.
To study the endocytic activity of dendritic cells (DCs) by obtaining fusion protein HSP70-EGFP as exogenous antigen and loading it with DCs derived from human peripheral blood.
The effect of lead, copper, mercury, and cadmium ions on peroxidase system activity in human peripheral blood neutrophils was studied using cytochemical methods.
Effects of chorionic gonadotropin (CG), estradiol, progesterone, and their physiological combinations on apoptosis of human peripheral blood T-lymphocytes were studied.
Human LAK cells were prepared by culturing normal human peripheral blood mononuclear cells (PBMC) with or without rIL-2 and assayed for T cell surface markers as well as anti-tumor activity against PGin vitro and in nude mice.
(3) EIF4E, VEGF and MMP-9 were expressed at high levels in Raji cells as compared to normal human peripheral blood monocular cells (NHPMC), and blockage of EIF4E expression brought down the expression of VEGF and MMP-9.
Our study showed that deguelin inhibited the proliferation of K562 cell and K562/ADM cell in a time- and dose-dependent manner and had minimal effects on normal human peripheral blood mononuclear cells.
Gp120 as a sole agent significantly suppressed IFN-γ production by mitogen-stimulated PBLs and induced the production of IFN-α in cultures of normal human peripheral blood mononuclear cells (PBMCs).
As different experimental models the leucocytes of the normal human peripheral blood have been used taking into account the relatively condensed chromatin of granulocytes with respect to that of the mononuclear cells.
However, with human whole blood, positive effects were seen using TCDO together with zymosan as well as specific and non-specific opsonizedKlebsiella pneumoniae K 17.
However, with human whole blood, positive effects were seen using TCDO together with zymosan as well as specific and non-specific opsonizedKlebsiella pneumoniae K 17.
These results correlate with observations from timekill curve studies in human whole blood, where ciprofloxacin showed superior bactericidal activity compared to norfloxacin or ofloxacin.