③Detection of mRNA expressions of LPS receptors on IECs: Total RNA was extracted from human normal IECs with and without LPS stimulation, human intestinal cancer epithelial cells (HIC), and THP1 cells, respectively.
③ 检测肠上皮细胞内毒素相关受体mRNA表达:提取有、无内毒素刺激的人正常肠上皮细胞及阳性对照THP1细胞的总RNA,用RT-PCR方法检测TLR4,TLR2,CD14和MD2 mRNA的表达,用RPA法检测TLR4,CD14和MD2 mRNA的表达。 用RT-PCR方法检测人小肠癌上皮细胞株(HIC)TLR4,CD14和MD2 mRNA的表达。
The FSCC is an important parameter in the calculation of crankshaft strength. It concerns the structure, size, material, technology and load of the crankshaft. The existing calculating formula of stress concentration coefficient (SCC) only represents the effect on the structure and size of the crankshaft.
Most notably, we found that the Cdx-2 in human intestine cell line Caco-2 is expressed in a differentiation-dependent manner and can efficiently bind to the mouse and human acat2 (acyl-coenzyme A: cholesterol acyltransferase 2) promoter regions, suggesting that the transcriptional factor Cdx-2 may play a role in regulating the acat2 expression in the intestinal cells.
This Research applies CT(computerized tomography) method to study the human body,mass center of Chinese adults. 40 male and 48 female Chinese adults were chosen as samples. In this wag,the regression equations for the calculation of the inertia parameters of human-body measurement;
Incubation of human intestinal carcinoma Caco-2 cells in suspension (i.e., in the absence of substrate contacts) leads to massive cell death by apoptosis.
The FSCC is an important parameter in the calculation of crankshaft strength. It concerns the structure, size, material, technology and load of the crankshaft. The existing calculating formula of stress concentration coefficient (SCC) only represents the effect on the structure and size of the crankshaft. There is a great error between the calculated value and the measured value, which influences the calculating precision of the crankshaft strength. The authors measure the actual SCC of the crankshaft thro...
Expression of c-myc oncogene of myeloma cells and their changes after hybridization in homo-and hetero-cellular cybrid and hybrid were studied with Northern-blot and dot-blot techniques, using cloned c-myc gene as a probe. Results indicated that c-myc oncogene transcripts could not be detected in cybrids of mouse X mouse (BW-RM), mouse X rabbit (BW-RR) and human X mouse (HMy-RM) hybridization. However, weak transcription of this oncogene was shown to be active in cybrids after 15th passages with a tendency ...
Ammoniun gulfate fractionation, N6-(6-aminohexyl)-5' AMP-Sepharoge affinity ohromatography and DEAE-Celluloge ion-exohange ohromatography were used to extract LDH-C4 from rat testis with a specific activity of 16.3 IU/mg, representing a 32.5% yield and about 1087-fold purification. The purified enzyme appeared to be homogeneous on polyaorylamide gel electrophoresis and a rabbit anti-rat LDH-C4 antigerum was prepared. BA-ELISA was used to examine the corss-reaotivity of the antiaerum with the extract of rat ...