助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   损伤基因 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

损伤基因
相关语句
  damage genes
     Survival rate, apoptosis rate, expression level of growth-inhibition and DNA damage genes GADD34, GADD45, GADD153 were measured using MTT,FCM and RT-PCR methods respectively.
     用流式细胞术分析不同浓度BPA对细胞凋亡的影响; 通过RT-PCR分析BPA引起的生长抑制、DNA损伤基因GADD45、GADD153、GADD34表达水平的变化。
短句来源
  “损伤基因”译为未确定词的双语例句
     Expression level of the three kinds of GADD genes increased at the concentration of 50 μmol/L BPA,but decreased at 100 μmol/L BPA+30 μg/L VitC group.
     生长抑制DNA损伤基因GADD45、GADD153.GADD34表达水平随着BPA浓度增加表达量也增加,100μmol/LBPA组显著高于正常对照组,但在100μmol/L,BPA+VitC组三种GADD基因表达量均稍降低。
短句来源
     Construction of plasmid vector pIRES2-EGFP-MyoD in gene therapy of muscle injury and its expression in mesenchymal stem cells
     肌损伤基因治疗中的质粒载体pIRES2-EGFP-MyoD的构建及其在MSCs中的表达
短句来源
     Cell cycle controlling gene p27,p15、 apoptosis related gene NDRG1,p8,STK3 and TP53INP1、 stress response gene GPX,p44 、 cell proliferation related gene and energy metabolism related gene all participated the antiapoptotic effect of PCF.
     细胞周期调控基因p27和p15、凋亡相关基因NDRGl,p8,STK3和TP53INP1、抗细胞损伤基因GPX和p44及细胞增殖和能量代谢相关基因均参与了PCF的抗凋亡作用。
短句来源
     The work is to observe changes of the GADD45 (Growth arrest and DNA damage gene 45, GADD45) gene mRNA expression after X-ray irradiation and to study the relationship between the GADD45 expression and irradiation dose. Peripheral blood was irradiated to 0-5 Gy by X-rays.
     观察生长抑制和DNA损伤基因45(Growth arrest and DNA damage gene 45,GADD45)X射线照射后表达的改变,并研究其与照射剂量之间的关系。
短句来源
     Gadd45a, a p53 and BRCA1-regulated growth arrest and DNA damage gene, plays important roles in suppressing cell transformation and tumor malignancy.
     Gadd45a,一个受p53和BRCA1调节的生长阻滞和DNA损伤基因,在抑制细胞转化和肿瘤恶性进展中扮演重要的角色.
短句来源
更多       
  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     The Evolution of Gene Therapy for Spinal Cord Injury
     脊髓损伤基因治疗的研究进展
短句来源
     Sports Joint Injury and Gene Therapy
     基因治疗与运动性关节损伤
短句来源
     Genetic Information Carrier Gene
     基因简史
短句来源
     Duodenal injuries
     十二指肠损伤
短句来源
查询“损伤基因”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


Objective:To investigate the gene expression of cfos in cerebrum at various time points after brain ischemiareperfusion and effect of nimodipine on the expression,providing evidence to elucidate the molecular mechanism of brain injury.Methods:The cerebrum was obtained in rats after complete global brain ischemiareperfusion.Total cellular RNAs were isolated by acid guanidine isothiocyanated extraction,and the RNAs were hybridized to 32Plabeled cDNA and analyzed by automatic system.Results:Expression of cfos...

Objective:To investigate the gene expression of cfos in cerebrum at various time points after brain ischemiareperfusion and effect of nimodipine on the expression,providing evidence to elucidate the molecular mechanism of brain injury.Methods:The cerebrum was obtained in rats after complete global brain ischemiareperfusion.Total cellular RNAs were isolated by acid guanidine isothiocyanated extraction,and the RNAs were hybridized to 32Plabeled cDNA and analyzed by automatic system.Results:Expression of cfos gene in cerebrum was rapidly induced by 20minute ischemia,it increased obviously at 20 minutes of reperfusion (A:10128±1454 vs.2132±0623,P<001),and it reached peak levels at 40 minutes of reperfusion (A:14908±1862).It started to decline markedly as early as 60 minutes of reperfusion,and it returned to the normal value recovered at 90 minutes of reperfusion.However,pretreatment with nimodipine prevented the gene expression of cfos at 40 minutes of reperfusion (A:2312±0941).Conclusions:These results indicate that cfos gene probably involves in the molecular mechanism of cerebrum damage during complete global brain ischemiareperfusion.

目的:研究大鼠全脑缺血再灌注时大脑细胞cfos基因表达及药物对其的影响,为探讨脑缺血再灌注损伤的基因机制提供依据。方法:从不同缺血再灌注时点大鼠的大脑提取RNA,与探针cfoscDNA进行打点分子杂交,图像经分析测出各时点大脑cfos基因mRNA的相对水平。结果:大脑cfos基因表达从缺血20分钟开始,再灌注20分钟表达水平显著高于对照组(A值:10.128±1.454比2.132±0.623,P<0.01),再灌注40分钟达高峰(A值:14.908±1.862),随后下降,再灌注90分钟达到并维持正常水平,显示cfos基因早期、快速、一过性高表达;用尼莫地平缺血前预处理能显著抑制缺血30分钟再灌注40分钟时大脑cfos基因表达(A值:2.312±0.941)。结论:cfos基因极可能介导了Ca2+的信息,作为信使参与全脑缺血再灌注大脑病理损伤的基因机制

Objective\ To study the effects of growth arrest and DNA damage(gadd) genes on the growth and apoptosis in ovarian cancer cell lines. Methods We constructed a retrovirus(pLXSN gadd153)which was transfected into SKOV3 and 3AO with lipofectin (DOTAP),respectively, then screened by anti G418 and identified the expression of gadd153 genes by RT PCR. Apoptosis was analyzed using FCM and DNA ladder. Results Growth inhibition and the apoptosis were observed in the SKOV3 cells transferred with gadd153 genes and...

Objective\ To study the effects of growth arrest and DNA damage(gadd) genes on the growth and apoptosis in ovarian cancer cell lines. Methods We constructed a retrovirus(pLXSN gadd153)which was transfected into SKOV3 and 3AO with lipofectin (DOTAP),respectively, then screened by anti G418 and identified the expression of gadd153 genes by RT PCR. Apoptosis was analyzed using FCM and DNA ladder. Results Growth inhibition and the apoptosis were observed in the SKOV3 cells transferred with gadd153 genes and also in the 3AO cells transferred with the same genes but there was only growth inhibition with no apoptosis occured. Conclusions The expression of gadd153 could make tumor cells arrested in G1/G0 and apoptosis could be developed in some other cell lines.

目的 研究生长抑制DNA损伤基因(grow th arrest and DNA dam age, gadd)对卵巢癌细胞生长抑制和凋亡的作用。 方法 RT-PCR鉴定卵巢癌SKOV3 和3AO细胞株gadd153 基因表达。通过目的基因的克隆、载体构建技术,将目的基因gadd153 重组于plXSN-neo 逆转录病毒表达载体;构建的pLXSN-gadd153载体采用脂质体(lipofectin)(DOTAP)的方法,分别转染SKOV3 和3AO细胞株;经G418 抗性筛选;RT-PCR表达鉴定;足叶乙甙(VP16)诱导,采用流式细胞仪分析(FCM)的方法检测转染前后肿瘤细胞的生长抑制和凋亡。 结果 SKOV3-gadd153 细胞生长抑制在G1/G0 期,部分细胞进入凋亡的状态;3AO-gadd153 细胞生长抑制,未引起凋亡。 结论 转染gadd153 基因的肿瘤细胞系,诱导表达后可诱发肿瘤细胞生长抑制在G1/G0期,并有细胞进入凋亡状态。证实gadd153 基因参与了细胞生长抑制和凋亡过程。

Objective:To study the effects of growth arrest and DNA damage(GADD)gene 45 on ovarian cancer cell lines and the relationship between the effect and the expression of p53 protein.Methods:Expressions of GADD45 and p53 protein in ovarian cancer cell liines SKOV3 and 3AO were identified respectively using RT PCR and Western Blot A eukaryotic expressing vector encoding GADD45 named pcDNA GADD45 was constructed and transfected into SKOV3 and 3AO cell...

Objective:To study the effects of growth arrest and DNA damage(GADD)gene 45 on ovarian cancer cell lines and the relationship between the effect and the expression of p53 protein.Methods:Expressions of GADD45 and p53 protein in ovarian cancer cell liines SKOV3 and 3AO were identified respectively using RT PCR and Western Blot A eukaryotic expressing vector encoding GADD45 named pcDNA GADD45 was constructed and transfected into SKOV3 and 3AO cell lines with lipofectin(DOTAP),then selected by anti G418 The expression of GADD45 was identified using RT PCR.Apoptosis was analyzed using FCM and DNA ladder.Results:The expression of GADD45 in ovarian cancer cell lines SKOV3 and 3AO was not detected.There was positive expression of p53 protein in SKOV3 whereas negative expression in 3AO.It was in the SKOV3 transferred GADD45 gene after induction by VP16 and UV irradiation that the apoptosis was found,but no apoptosis in the 3AO transferred the same gene.Conclusions:The cell line,which has positive expression of p53 protein transferred GADD45 gene shows the apoptosis after induction by VP16 and UV irradiation.The cell line,however,which has negative expression of p53 protein,couldn

目的:研究生长抑制DNA损伤基因(growtharestandDNAdamage,GADD)对卵巢癌细胞的作用及与p53蛋白表达的关系。方法:利用RT-PCR鉴定卵巢癌SKOV3和3AO细胞株GADD45的表达;采用RT-PCR和WesternBlot鉴定SKOV3和3AO细胞株p53蛋白的表达。通过载体构建将目的基因GADD45重组于pcDNA-Ⅲ真核质粒内。利用脂质体(DOTAP)法转染SKOV3和3AO细胞株;G418抗性筛选,RT-PCR进行表达鉴定。采用流式细胞仪分析(FCM)以及DNA梯度法对细胞凋亡现象进行检测。结果:卵巢癌SKOV3和3AO细胞株GADD45表达阴性。SKOV3细胞株p53蛋白表达阳性;3AO细胞株p53蛋白表达阴性。转染GADD45基因的SKOV3细胞株经化学药物足叶已甙(VP16)和紫外线诱导后,可以使该细胞产生凋亡现象,而对转染GADD45基因的3AO细胞株经诱导后,未见细胞凋亡现象。结论:转染GADD45基因,对p53蛋白表达阳性的细胞株SKOV3经诱导后,可使细胞进入凋亡状态。对p53蛋白表达阴性的细胞株3AO转染GADD45基因后,经诱导细胞无凋亡现象,...

目的:研究生长抑制DNA损伤基因(growtharestandDNAdamage,GADD)对卵巢癌细胞的作用及与p53蛋白表达的关系。方法:利用RT-PCR鉴定卵巢癌SKOV3和3AO细胞株GADD45的表达;采用RT-PCR和WesternBlot鉴定SKOV3和3AO细胞株p53蛋白的表达。通过载体构建将目的基因GADD45重组于pcDNA-Ⅲ真核质粒内。利用脂质体(DOTAP)法转染SKOV3和3AO细胞株;G418抗性筛选,RT-PCR进行表达鉴定。采用流式细胞仪分析(FCM)以及DNA梯度法对细胞凋亡现象进行检测。结果:卵巢癌SKOV3和3AO细胞株GADD45表达阴性。SKOV3细胞株p53蛋白表达阳性;3AO细胞株p53蛋白表达阴性。转染GADD45基因的SKOV3细胞株经化学药物足叶已甙(VP16)和紫外线诱导后,可以使该细胞产生凋亡现象,而对转染GADD45基因的3AO细胞株经诱导后,未见细胞凋亡现象。结论:转染GADD45基因,对p53蛋白表达阳性的细胞株SKOV3经诱导后,可使细胞进入凋亡状态。对p53蛋白表达阴性的细胞株3AO转染GADD45基因后,经诱导细胞无凋亡现象,证实GAD?

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关损伤基因的内容
在知识搜索中查有关损伤基因的内容
在数字搜索中查有关损伤基因的内容
在概念知识元中查有关损伤基因的内容
在学术趋势中查有关损伤基因的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社