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细胞制备
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  cell preparation
     Using misgurnus auguillicaudatus as material, we made a primary study of embryonic cell preparation, cryopreservation, thawing and primary cell culture.
     另一个方面的有关原理技术,本文主要以泥鳅胚胎细胞为材料,根据细胞冻存,进行泥鳅胚胎细胞制备、冷冻保存、解冻、原代培养研究。
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     The optimum conditions of Streptomyces sp.108 immobilized cell preparation are reported in this paper.
     报道了链霉菌(Streptomycessp.108)固定化细胞制备的最适条件。
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     The optimal optical density (OD_ 600) range for competent cell preparation varied for each of the strains.
     每种菌株的感受态细胞制备的最佳光密度(OD600值)不同.
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     An improved method of cell preparation for cell cycle assay
     一种改进的用于测定细胞周期的细胞制备方法
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     CONCLUSION Serious bacterial contamination is found in the procedures of islet cell preparation.
     结论:胰岛细胞制备过程中细菌性污染严重。
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  cells preparation
     Experimental observation on the effect of five herbs in T-lymphocyte transformation and lymohocine activated killer cells preparation
     T淋巴细胞转化和LAK细胞制备中五种中药效用的实验观察
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  “细胞制备”译为未确定词的双语例句
     Results: Liposome-mediated pCA13-VEGI_(151) and pJM17 cotransfection of 293A cells (through homologous recombination of intracellular plasmid) is a practical and feasible method to obtain high-titre recombinant adenovirus.
     结果:应用细胞内质粒DNA同源重组法,将脂质体介导质粒pCA13-VEGI151与pJM17共转染293A细胞制备重组腺病毒,所获病毒滴度高,是一种制备重组腺病毒切实可行的方法。
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     Preparation and application of the epidemic encephalitis hemagglutinin from the infected C 6/36 cells
     C6/36细胞制备乙型脑炎血凝抗原的方法和应用效果
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     Human CD3AK cells were prepared from peripheral blood mononuclear cells by culturing with recombinant IL-2 and antiCD3AK McAb.
     应用抗CD3单抗和人rIL-2共同诱导人外周血单个核细胞制备人CD3AK细胞(CDAK).
短句来源
     Methods Total protein from BEP2D and BERP35T-2 cells was extracted and analyzed by 2-D electrophoesis and peptide mass fingerprinting spectrum.
     方法 培养BEP2D和BERP35T 2细胞 ,制备细胞总蛋白 ,进行双相电泳和质谱分析 ;
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     The preparation of fructose 1,6-diphosphate (FDP) by immobillized yeast cells and its production were studied.
     本文研究了固定化酵母细胞制备果糖1,6二磷酸(FDP)的方法及其生产。
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  cell preparation
Vegetative growth of thalli is sharply inhibited at the stage of cell preparation to gametogenesis a day before the beginning of gamete formation.
      
We therefore investigated the impact of adenoviral S100A1 overexpression on fractional shortening (FS%) and systolic Ca2+ transients in adult rat cardiomyocytes as well as of S100A1 protein on SERCA activity in skinned cell preparation.
      
While DNA was not recovered from samples at the 200 kGy exposure level, protein activity was reduced by 19 to 78%, depending on the method of cell preparation.
      
Methods of dendritic cell preparation for acute lymphoblastic leukemia immunotherapy in children
      
Whole cell preparation of original LC, susceptible to bacteriophages SG-T and J1, was more effective than its mutants resistant to either bacteriophage.
      
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  cells preparation
A two stage process is applied for the fine tuning of the CuGaSe2 composition and electronic properties appropriate for the solar cells preparation.
      
Homotypic adhesion of Caco-2 single cells to attached cells Preparation of Caco-2 single cell suspension.
      


The peritoneal exudate macrophages were prepared by injecting a suspension of Sephadex G-50 into peritoneal cavity of mice and cAMP concentrations of exudate macro-phages in the thymosin-treated mice and in control were determined separately. The value in control ranged between 0.31-1.23PM/10~6 cells (M±SD = 0.77±0.46 ), and that in the thymosin-treated mice was much higher (P<0.05) . In view of the fact that thymosin was capable of activating macrophages and significantly increasing cAMP concentration in exudate...

The peritoneal exudate macrophages were prepared by injecting a suspension of Sephadex G-50 into peritoneal cavity of mice and cAMP concentrations of exudate macro-phages in the thymosin-treated mice and in control were determined separately. The value in control ranged between 0.31-1.23PM/10~6 cells (M±SD = 0.77±0.46 ), and that in the thymosin-treated mice was much higher (P<0.05) . In view of the fact that thymosin was capable of activating macrophages and significantly increasing cAMP concentration in exudate macrophages, we believe that by determination of cAMP concentration in macrophages the functional states of macrophages can be revealed, and it may be used as an index for further studies of macrophage activation and immunological stimulant as well.

我们报告了采用SephadexG-50是液诱发小白鼠腹腔渗出液巨噬细胞的制备方法,并测定了注入胸腺素(F5)和正常小白鼠腹腔渗出液巨噬细胞内cAMP的含量.对照小白鼠巨噬细胞内cAMP含量为0.31—1.23PM/10~6细胞(M±SD0.77±0.46PM/10~6),而接受胸腺素的小白鼠表现明显增高(P<0.05).鉴于胸腺素(F5)有活化巨噬细胞及增加其cAMP含量的事实,因而我们认为测定巨噬细胞内cAMP的含量能揭示其功能状态,也可作为研究巨噬细胞活化或免疫刺激剂的指标.

Comparative chromosome studies were carried out by means of short culture of lymphocytes from peripheral blood of 8 cows in which leucosis showed haematologically and serologically negative, 8 with persistent lymphocytosis and 2 with bovine lymphosarcoma. The results of the studies revealed that aberration took place in different degrees in the lymphocyte chromosomes of a11 the cases with lymphocytosis. Diploid accounted for 68.9—83.9% of the total number of cells in the negative cases, averaging 76.6%, and...

Comparative chromosome studies were carried out by means of short culture of lymphocytes from peripheral blood of 8 cows in which leucosis showed haematologically and serologically negative, 8 with persistent lymphocytosis and 2 with bovine lymphosarcoma. The results of the studies revealed that aberration took place in different degrees in the lymphocyte chromosomes of a11 the cases with lymphocytosis. Diploid accounted for 68.9—83.9% of the total number of cells in the negative cases, averaging 76.6%, and 32,4—58.7% in the cases with lymphocytosis, 46.3% on an average. There was an extremely marked difference between them (P<0.01). In most of the cases with lymphocytosis the proportions of hypodiploid and hyperdiploid were higher than those in the negative cases. The increases in the proportions of hypodiploid were notabler than those in hyperdiploid. By comparison with the cases with persistent lymphocytosis, the proportions of diploid in the cases with lymphosarcoma presented a further decrease trend (28.6% and 34.4% respectively), the increases of the proportion of hyperdiploid being notabler in aneuploid, The increase of the proportion of hypodiploid approached that of hyperdiploid in one case, The proportion (42.9%) of diploid in the other case with lymphosarcoma whose chromosome specimen was prepared with marrow cells was higher than that in the two cases with blood lymphocytes the aneuploids in this case being mostly hyperdiploids. The frequency of structural aberration of chromosomes in the haematologically and serologically negative cases, cases with persistent lymphocytosis and cases with lymphosarcoma was 2.1%, 5.2% and 8% of the cells respectively. The chromosome aberrations of cows with leucosis presented a phasic nature at the two stages of persistent lymphocytosis and lymphosarcoma.A similar abnormal chromosome was found in nearly half of the cells in metaphase in two cases with persistent lymphocytosis.An abnormal clone was discovered in a case with lymphosarcoma. One chromosome of the 29th pair was deformed, and the other was lost. According to the researches of predecessors and the results of this experiment a tentative idea was advanced on the possibility of the existence of marker chromosome in lymphosarcoma.

采用外周血淋巴细胞短期培养法对8例血液学检查及琼扩实验阴性牛、8例持续性淋巴细胞增生牛和2例淋巴肉瘤牛进行的染色体比较研究表明,造白细胞组织增生牛的淋巴细胞染色体都发生了不同程度的畸变。阴性牛二倍体细胞的比例处于68.9%—83.9%之间,平均为76.6%;持续性淋巴细胞增生牛二倍体细胞的比例处于32.4%—58.7%之间,平均为46.3%,二者之间有极显著差异(P<0.01)。大部分持续性淋巴细胞增生病例亚二倍体和超二倍体细胞的比例都较阴性牛增高。亚二倍体细胞增多比超二倍体显著。与持续性淋巴细胞增生牛比较,淋巴肉瘤牛二倍体细胞的比例呈现出进一步降低趋势(分别为28.6%和34.4%),非整倍体细胞的增加以超二倍体细胞比例的增高较为显著,有1例亚二倍体比例的增高和超二倍体接近。采取骨髓细胞制备染色体标本的另一例淋巴肉瘤牛二倍体细胞的比例比采取外周血的两例要高(42.9%),非整倍体细胞多半为超二倍体细胞。阴性牛、持续性淋巴细胞增生牛和淋巴肉瘤牛染色体形态变异的频率分别为2.1%,5.2%和8%。造白细胞组织增生牛的染色体畸变在持续性淋巴细胞增生和淋巴肉瘤这两个不同时期存在着阶段性变化。 另外两例持...

采用外周血淋巴细胞短期培养法对8例血液学检查及琼扩实验阴性牛、8例持续性淋巴细胞增生牛和2例淋巴肉瘤牛进行的染色体比较研究表明,造白细胞组织增生牛的淋巴细胞染色体都发生了不同程度的畸变。阴性牛二倍体细胞的比例处于68.9%—83.9%之间,平均为76.6%;持续性淋巴细胞增生牛二倍体细胞的比例处于32.4%—58.7%之间,平均为46.3%,二者之间有极显著差异(P<0.01)。大部分持续性淋巴细胞增生病例亚二倍体和超二倍体细胞的比例都较阴性牛增高。亚二倍体细胞增多比超二倍体显著。与持续性淋巴细胞增生牛比较,淋巴肉瘤牛二倍体细胞的比例呈现出进一步降低趋势(分别为28.6%和34.4%),非整倍体细胞的增加以超二倍体细胞比例的增高较为显著,有1例亚二倍体比例的增高和超二倍体接近。采取骨髓细胞制备染色体标本的另一例淋巴肉瘤牛二倍体细胞的比例比采取外周血的两例要高(42.9%),非整倍体细胞多半为超二倍体细胞。阴性牛、持续性淋巴细胞增生牛和淋巴肉瘤牛染色体形态变异的频率分别为2.1%,5.2%和8%。造白细胞组织增生牛的染色体畸变在持续性淋巴细胞增生和淋巴肉瘤这两个不同时期存在着阶段性变化。 另外两例持续性淋巴细胞增生牛的近半数中期细胞都出现了一条相似的异常染色体。 发现在1例淋巴肉?

Substitution of thymidine with 5-Bromo-deoxyuridine (BUdR) was performed in vivo in mice for the detection of gossypol effects on the chromosome aberration and sister chromatid exchange (SCE) in spermatogonia. The male Kun-ming mice received a daily dose of 4mg/kg of gossypol for two weeks, followed by a single injec- tion intraperitoneally of BUdR adsorbed on activated charcoal. Metaphases from spermatogonia were prepared for observation 54 hours after the injection.

本实验用BUdR替代胸腺嘧啶核苷的体内实验方法检验棉酚对小鼠精原细胞姐妹染色单体交换(SCE)频率的影响。雄性昆明小鼠连续口服棉酚(4毫克/公斤/日)2周,随之一次腹腔注射吸附于活性炭上的BUdR。经过54小时后,取精原细胞制备中期染色体进行观察。实验结果表明,服棉酚组小鼠精原细胞染色体的畸变率和SCE频率均与对照组无明显差别。而作为阳性对照的致突剂丝裂霉素C(Mitomycin C)所诱致的SCE则明显增高。因而从生殖细胞SCE达一敏感指标提供了证据支持已有报道中认为棉酚不是致突变剂的观点。对所应用以分析精原细胞SCE的体内方法及其在检查避孕药物遗传效应上的意义予以了讨论。

 
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