After addition of small amounts of Ca~(++) (Ca~(++)/EGTA=0.56, free Ca~(++)=1.22×10~(-7) mol/L) to platelet lysate rich in actin and myosin and containing other platelet cytoskeletal components, a precipitate formed immediately.
Incubation of lysates with the calmodulin inhibitor chlorpromazine or trifluoperazine (50 μmol/L) at 37℃ for 30 min neither prevented precipitate formation after addition of Ca~(++) nor influenced the turbidity Ca~(++) concentration curves and the time course of complex formation.
After platelet lysis by 1% Triton X-100 and the separation of lipid rafts on a discontinuous sucrose gradient, TIIICBP is recovered in lipid raft-containing fractions and Triton X-100 insoluble fractions enriched in cytoskeleton proteins.
Activation of complement also occurs as plasmin activates C1 resulting in red cell and platelet lysis.
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This concentration was shown to have no effect on either platelet aggregation induced by agonists or platelet lysis.
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There was no increase in lactate dehydrogenase, ruling out platelet lysis, and moreover PGE1 was inhibitory.
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After addition of small amounts of Ca~(++) (Ca~(++)/EGTA=0.56, free Ca~(++)=1.22×10~(-7) mol/L) to platelet lysate rich in actin and myosin and containing other platelet cytoskeletal components, a precipitate formed immediately. Analysis of this precipitate by SDS-PAGE showed that the precipitated complex was composed of actin, myosin and small amounts of unidentified proteins. The precipitate formation was Ca~(++) concentration dependent. The turbidities of lysates after addition of Ca~(++) increased gradu...
By setting up in vitro an antibody-antigen-complement system andmeasuring platelet ~(51)Cr release in the system,we investigated the mecha-nism of platelet destruction in patients with idiopathic thrombocytopenicpurpura (ITP).The results showed that the values of ~(51)Cr release in 45patients with ITP were much higher than those in the 30 normals (P<0.01).There was no difference in the value between the patients of theacute and the chronic.In 31/45 cases the values of ~(51)Cr release were abovethe upper lim...
Seven dogs are inflicted with severe combined burn-blast injury and 6 as the controls. The counts?functions and structural changes of platelets and megaka-ryocytes are studied. Observations of platelets' peripheral venous blood platlets diminish significantly during 1-5 d postinjury(PI). Adhesive function reduces during 3-7 d PI. Various structural abnromalities are found. Degenerated mega-karyocytes increase (P<0.01) and 1%-5% of megakaryocytes are phagocytized by neutrophils (megakaryocytophagia) through ...