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  using enzyme
     ESTABLISHMENT OF A RAPID DOT-ELISA METHED FOR IDENTIFYING HUMAN SEMINAL STAIN BY USING ENZYME LABLED ANTI-HUMAN SEMEN MONOCLONAL ANTIBODY(A_(10)C_6)
     用酶标抗人精液单克隆抗体A_(10)C_6建立ELISA斑点法快速鉴定人精(液)斑
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     The contents of IAA、GA1+3、ABA and iPAs of cotton ovules colleced on -3,-1,0,+1,+3,+5,+8,+12 days after anthesis in 5 types of Lint and fuzz mutants were examined by using Enzyme Linked Immuno Sorbent Assay(ELISA).
     用酶联免疫法分别测定了开花前3d、1d,开花当天,开花后1、3、5、7、8、12d的5个棉纤维突变体胚珠中GA1+3、IAA、ABA及iPAs的含量。
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     Using enzyme immunoassay, 686 urinary HCG of 298 patients with acute gynaecological diseases were tested from January 1989 to December 1990. Among them 47 cases of extrauterine pregnancy and 71 cases of threatened abortion yielded positive results.
     1989年1月~1990年12月,用酶标免疫检验法,对298例(686次)妇科急症病人尿中绒毛膜促性腺激素(HCG)检验中,筛查出宫外孕47例,先兆流产71例,均得到及时处理。
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     Methods Short chain oligonucleotide was designed according to the TGF-β1 mRNA sequence provided by Genebank, then DNA segment was gained through annealing after chemosynthesis, and then was cloned to pWH1 vector. The recombinant TGF-β1 shRNA expression vector was evaluated by using enzyme cutting.
     方法根据Genebank提供的TGF-β1 mRNA序列,设计短链寡核苷酸,化学合成后经退火形成双链 DNA片段,克隆到pWH1载体中,用酶切方法对重组体进行鉴定:最后将构建的TGF-β1特异性shRNA表达载体转染涎腺粘液表皮样癌细胞,通过RT-PCR、免疫组化观察其对细胞TGF-β1 mRNA和蛋白水平表达的影响。
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     The Synthesis of Isoamyl Acetate Using Enzyme as Catalyst
     用酶作催化剂合成乙酸异戊酯
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  “用酶”译为未确定词的双语例句
     Study on the Methodology of Evaluating Duck Feedstuffs Metabolizable Energy with Enzymic Method
     用酶法评定鸭饲料代谢能的方法学研究
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     DETERMINATION OF EB VIRUS ANTIBODY (VCA-IgA) IN SERA OF PATIENTS WITH NASOPHARYNGEAL CANCER BY IMMUNOENZYMATIC ASSAY
     用酶免疫法检测血清中EB病毒抗体(VCA-IgA)诊断鼻咽癌
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     Detection of anti-dsDNA antibody in SLE patients by enzyme marking staphylococcal protein A (SPA)
     用酶标记金葡菌A蛋白检测SLE患者抗dsDNA抗体的研究
短句来源
     DETECTION OF ANTI-dsDNA ANTIBODY IN SLE BY IMMUNOHISTO-CHEMICAL METHOD WITH ENZYME MARKING McAb
     用酶标单克隆抗体免疫组化法检测SLE抗dsDNA抗体的研究
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     Practial application of feed cnzymcs
     猪、禽饲用酶制剂的实际应用(下)
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  相似匹配句对
     The activation en ergy and reaction order are calculated by G.
     G.
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     Enzyme Preparations Used for Feeds
     饲料制剂
短句来源
     melanogaster, C.
     C.
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     Application Advancement of Feed Enzyme
     饲进展
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     Enzyme mimics
     模型
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  using enzyme
Degradation of 4-aminophenol by hydrogen peroxide oxidation using enzyme from Serratia marcescens as catalyst
      
Atrazine consumption by liquid surface cultures of Mycelia sterilia INBI 2-26 was monitored by using enzyme immune assay and reversed-phase HPLC.
      
Some catalytic and regulatory properties of NADP-IDH were studied using enzyme preparations purified from normal plants and plants exposed to salt stress.
      
Using enzyme-linked immunosorbent analysis, we detected zeatin (riboside) in the culture liquid of both bacteria studied.
      
Using enzyme-linked immunosorbent assays, the antibody activity of the monoclonal igM was shown to be directed against GD1a ganglioside, a new and so far unreported specificity.
      
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(1) Sodium salt of reduced codehydrogenase I has been obtained in good yield as a dry powder from codehydrogenase I by reduction with alcohol and alcohol dehydrogenase. This preparation was stable for at least 5 months when kept dry at -15℃. (2) The properties of the particle-bound codehydrogenase I cytochrome reductase system in heart muscle preparation were found to differ considerably from those of the soluble enzyme as obtained by Mahler et al. Among other things, the affinity for cytochrome c of the particle-bound...

(1) Sodium salt of reduced codehydrogenase I has been obtained in good yield as a dry powder from codehydrogenase I by reduction with alcohol and alcohol dehydrogenase. This preparation was stable for at least 5 months when kept dry at -15℃. (2) The properties of the particle-bound codehydrogenase I cytochrome reductase system in heart muscle preparation were found to differ considerably from those of the soluble enzyme as obtained by Mahler et al. Among other things, the affinity for cytochrome c of the particle-bound enzyme is much greater than the soluble enzyme. The Michaelis constant for cytochrome c of the former is only one twelfth of that of the latter.(Fig. 2A). (3) With either oxygen or excess cytochrome c as electron acceptor, it was found that the overall activity, in terms of rate of oxygen consumption or cytochrome c reduction, when both succinate and reduced codehydrogenase I were oxidized simultanously, did not represent the sum of the rates of oxidation when these two substrates were separately oxidized but equalled only the faster of the two separate oxidation rates(Fig. 5, Tables 1, 2). If 2,6-dichlorophenol indophenol was used as the electron acceptor, the overall rate of simultaneous oxidation of these two substrates was found to equal exactly the sum of the rates of separate oxidation(Table 3). (4) When either oxygen or excess cytochrome c was used as the electron acceptor, reduced codehydrogenase I and succinate each inhibited the rate of oxidation of the other(Figs 4, 6 & 7). Evidence has been presented to show that the inhibition of succinate oxidation by reduced codehydrogenase I is not due to the accumulation of oxaloacetate. (5) When malonate was also added to the reaction mixture, succinate no longer produced any inhibition of the oxidation of reduced codehydrogenase I(Fig. 8). (6) It is therefore concluded that in heart muscle preparation both succinate and reduced codehydrogenase I are oxidized by cytochrome c through a common, velocity limiting factor. This is in accordance with the view previously reached by some workers from studies on the action of certain inhibitors. However, it should be noted that in our experiments no agents which might produce any conceivable change in the colloidal structure of the enzyme system has been employed. (7) It should be emphasized that our results clearly show that great caution must be exercised in drawing conslusion on the role an enzyme might play in a complex enzyme system from studies of the properties of a solubilized enzyme. (8) It is believed that the competition of two enzyme systems for a common linking factor as demonstrated in this report has provided a new method for studies on the mutual relations of two or more enzyme systems.

(一)本報告提供了一個從輔酶Ⅰ,用酶還原法製備還原輔酶Ⅰ的方法。我們所製得的還原輔酶Ⅰ鈉鹽乾粉,可以在低温保存數月而不被氧化。 (二)與心肌製劑中顆粒相結合的輔酶Ⅰ細胞色素還原酶系,和用乙醇抽出的水溶性的輔酶Ⅰ細胞色素還原酶的性質頗不相同。其中比較重要的不同點是對於細胞色素c的親力,前者遠大於後者,其米氏常數僅約為後者的十二分之一。 (三)用一心肌顆粒製劑作為材料,無論用氧或過量之細胞色素c作為氫受體,還原輔酶Ⅰ與琥珀酸同時氧化時的總速度,不等於二者分別氧化時速度之和,而僅等於其中氧化較快者單獨氧化時之速度。但如用[2,6]二氯靛酚作為氫受體時,二者共同氧化時之總速度完全等於二者分別氧化時速度的和。 (四)當用氧或過量之細胞色素c作為氫受體時,琥珀酸與還原輔酶Ⅰ能彼此互相抑制對方氧化的速度。有足夠的實驗材料說明,還原輔酶Ⅰ對於琥珀酸氧化的抑制,不是由於草醯乙酸聚集的緣故。 (五)如果在反應混合物中同時含有琥珀酸脫氫酶的專一抑制劑,丙二酸,則琥珀酸對於還原輔酶Ⅰ氧化作用的抑制即被解除。 (六)根據以上的實驗結果,可以認為,還原輔酶Ⅰ及琥珀酸先通過一個共同的因子與細胞色素c作用。這個共同的因子在一般情...

(一)本報告提供了一個從輔酶Ⅰ,用酶還原法製備還原輔酶Ⅰ的方法。我們所製得的還原輔酶Ⅰ鈉鹽乾粉,可以在低温保存數月而不被氧化。 (二)與心肌製劑中顆粒相結合的輔酶Ⅰ細胞色素還原酶系,和用乙醇抽出的水溶性的輔酶Ⅰ細胞色素還原酶的性質頗不相同。其中比較重要的不同點是對於細胞色素c的親力,前者遠大於後者,其米氏常數僅約為後者的十二分之一。 (三)用一心肌顆粒製劑作為材料,無論用氧或過量之細胞色素c作為氫受體,還原輔酶Ⅰ與琥珀酸同時氧化時的總速度,不等於二者分別氧化時速度之和,而僅等於其中氧化較快者單獨氧化時之速度。但如用[2,6]二氯靛酚作為氫受體時,二者共同氧化時之總速度完全等於二者分別氧化時速度的和。 (四)當用氧或過量之細胞色素c作為氫受體時,琥珀酸與還原輔酶Ⅰ能彼此互相抑制對方氧化的速度。有足夠的實驗材料說明,還原輔酶Ⅰ對於琥珀酸氧化的抑制,不是由於草醯乙酸聚集的緣故。 (五)如果在反應混合物中同時含有琥珀酸脫氫酶的專一抑制劑,丙二酸,則琥珀酸對於還原輔酶Ⅰ氧化作用的抑制即被解除。 (六)根據以上的實驗結果,可以認為,還原輔酶Ⅰ及琥珀酸先通過一個共同的因子與細胞色素c作用。這個共同的因子在一般情形之下,也是這兩個酶系統的速度限制因子。應該指出在我們的實驗中,並未使用任何可能影響酶系統結構的條件,因此我們的結果是在一個比較接近於生理狀態的情形之下獲得的。 (七)應該着重指出,從本報告的結果可以看到,一個用人為的方法從複雜酶系上溶解下來的酶的性質,有時並不能代表這個酶在有組織的酶系統中的真實情况。 (八)我們相信,本報告所說明的兩酶系競爭一個共同因子的一些現象,將为研究複雜酶系之間的相互關係,提供一個新的方法。

Several important factors for the enzymatic synthesis of poly C were studied and the proper reaction conditions for the enzymatic synthesis of poly C were given. The polymerization ratio of poly C synthesis is 72.4%. The sedimentation constant of poly C is 8.8 S.In the present paper, the relationship among the nine factors at four orders were studied and optimal reaction conditions for the enzymatic synthesis of poly I were obtained by orthogonal method. The polymerization ratio of poly I synthesis is 72.0%....

Several important factors for the enzymatic synthesis of poly C were studied and the proper reaction conditions for the enzymatic synthesis of poly C were given. The polymerization ratio of poly C synthesis is 72.4%. The sedimentation constant of poly C is 8.8 S.In the present paper, the relationship among the nine factors at four orders were studied and optimal reaction conditions for the enzymatic synthesis of poly I were obtained by orthogonal method. The polymerization ratio of poly I synthesis is 72.0%. Control of the molecular size of poly I was also studied; after the maximum relative viscosity of the reaction solution was reached, the reaction was stopped The sedimentation constant of poly I is 11 S.The characteristics of the above reactions are as follows: (1) only crude PNPase from E. coli. is needed, (2) small amounts of the enzyme are used and (3) the yield of the polymers is high. Cu~(++) and Mn~(++) ions are used in synthesizing poly I, which can attain a high molecular weight even with the crude enzyme. These methods provide an effective procedure for the synthesis of poly I: C for medical uses.

本文报道了影响酶促合成poly C的主要因素,找出了合成poly C的适宜反应条件。聚合率达72.4%,poly C的沉降常数为8.8S。采用正交设计法研究了影响酶促合成poly I的九个因素在四个位级上的相互关系,并求出了合成poly I的最适反应条件。聚合率为72.0%。同时还研究了控制poly I分子大小的途径;当反应系统相对粘度显最大值后中止反应,此时poly Ⅰ的沉降常数为11S。上述反应的特点是利用大肠杆菌的PNPase粗酶液,而且用酶量少,产率高。在合成poly I时运用了Ca~(++)和Mn~(++)离子的特殊作用,达到了用粗酶液合成大分子产物的目的。实践证明,这些方法为生产药用poly I:C提供了可行的工艺路线。

Several important factors for the enzymatic synthesis of poly C were studied andthe proper reaction conditions for the enzymatic synthesis of poly C were given. Thepolymerization ratio of poly C synthesis is 72.4%. The sedimentation constant of polyC is 8.8S.In the present paper, the relationship among the nine factors at four orders werestudied and optimal reaction conditions for the enzymatic synthesis of poly I wereobtained by orthogonal method. The polymerization ratio of poly I synthesis is 72.0%.Control...

Several important factors for the enzymatic synthesis of poly C were studied andthe proper reaction conditions for the enzymatic synthesis of poly C were given. Thepolymerization ratio of poly C synthesis is 72.4%. The sedimentation constant of polyC is 8.8S.In the present paper, the relationship among the nine factors at four orders werestudied and optimal reaction conditions for the enzymatic synthesis of poly I wereobtained by orthogonal method. The polymerization ratio of poly I synthesis is 72.0%.Control of the molecular size of poly I was also studied: after the maximum relativeviscosity of the reaction solution was reached, the reaction was stopped Thesedimentation constant of poly I is 11S.The characteristics of the above reactions are as follows:(1)only crude PNPasefrom E. coli. is needed, (2)small amounts of the enzyme are used and (3)the yield ofthe polymers is high. Cu~(++) and Mn~(++) ions are used in synthesizing poly I, which canattain a high molecular weight even with the crude enzyme. These methods providean effective procedure for the synthesis of poly I:C for medical uses.

本文报道了影响酶促合成poly C 的主要因素,找出了合成poly C 的适宜反应条件。聚合率达72.4%,poly C 的沉降常数为8.8S。采用正交设计法研究了影响酶促合成poly I 的九个因素在四个位级上的相互关系,并求出了合成poly I 的最适反应条件。聚合率为72.0%。同时还研究了控制poly I 分子大小的途径;当反应系统相对粘度显最大值后中止反应,此时poly I 的沉降常数为11S。上述反应的特点是利用大肠杆菌的PNPase 粗酶液,而且用酶量少,产率高。在合成poly I时运用了Ca~(++)和Mn~(++)离子的特殊作用,达到了用粗酶液合成大分子产物的目的。实践证明,这些方法为生产药用poly I:C 提供了可行的工艺路线。

 
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