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诱导凋亡能力
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  apoptosis-induced ability
     One of the possible reasons for the enhanced apoptosis-induced ability may be that NF-κB activation is inhibited in tumor cells treated with mt 471rhTNF-α.
     肿瘤细胞内NF-κB活化明显受抑是mt471rhTNF-α诱导凋亡能力增强的主要原因之一.
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  “诱导凋亡能力”译为未确定词的双语例句
     This study was to evaluate in vitro antitumor effects of different chemotherapeutic drugs on fresh human gast ric cancer cells, and explore their relationships with expressions of P-glycopro tein (P-gp), and glutathione S transferase -π (GST-π) in human gastric cancer tissue.
     本研究旨在评价不同化疗药物对原代胃癌细胞的体外杀伤效应及其诱导凋亡能力,同时研究上述效应与胃癌组织P鄄糖蛋白(P鄄gp)和谷胱甘肽S转移酶π(GST鄄π)表达的关系。
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     MethodsUsing RT-PCR,immunohistochemistry and reverse-phase high-performance liquid chromatography(HPLC) assay,CYP3A5 mRNA protein and activities in leukemia cell lines were detected. Daunorubicin(DNR) sensitivity profiles of leukemia cell lines were obtained with MTT assay. Cell cycle characteristics and apoptosis induced by DNR were observed with flow cytometry (FCM) analysis.
     方法逆转录 PCR、免疫组化、高压液相色谱法检测白血病细胞系CYP3A5基因的转录、表达及活性 ,四氮唑蓝法检测细胞系对柔红霉素 (DNR)IC50 值、流式细胞仪 (FCM )检测细胞周期及DNR诱导凋亡能力
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     Methods Using flow cytometry and fluorescence microscopy,we investigated in vitro BEL-7402 cells in different conditions.
     方法 体外培养的BEL -740 2细胞分别经单纯热疗,5 -氟尿嘧啶、丝裂霉素单纯化疗,或二者合并热处理,利用MTT、荧光显微镜和流式细胞仪,研究热化疗对细胞毒性,诱导凋亡能力和细胞周期的影响。
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     Conclusions: Arsenic trioxide of low concentration combining with cisplatin of low concentration may increase the anti-osteosarcoma effect, compare to use of arsenic trioxide or cisplatin of high concentration respectively, either in inhibiting colon26 cells proliferation or inducing apoptosis.
     结论:低浓度As2O3和CDP联合应用与两药高浓度单独应用相比,对colon26细胞增殖的抑制作用和诱导凋亡能力均有明显增强。
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     The results also suggested that pueraria flavonoids extract may execute apoptosis through the Fas way and/or mitochondria way.
     说明葛根黄酮提取物诱导HL-60细胞凋亡可能通过经F as死亡受体和/或线粒体途径共同介导执行,葛根黄酮提取物抑制诱导凋亡能力强于葛根素和大豆甙元单体。
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  相似匹配句对
     Finally, lead to the articular cartilage
     诱导软骨细胞凋亡
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     The morphology of apoptosis cells were observed with conventional Giemsa staining and DAPI counterstained.
     诱导瘤细胞的凋亡及分化;
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     scavenging ability of BSCA were measured by the chemical simulation.
     能力;
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     ( P<0.05)(2) The FYK can predominantly induce apoptosis of neoplastic cells and strengthen phagocytic function of macrophagus.
     (2)FYK 显著诱导瘤细胞凋亡,增强巨噬细胞的吞噬能力;
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     Its powerful capability of inducing apoptosis and its special selectivity of killing tumor cells have raised the interests of many researchers.
     TRAIL强大的凋亡诱导能力和选择性的杀伤作用 ,引起了广大研究者极大的兴趣。
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To study taxo1-induced apoptosis and growth inhibition in human esophageal carcinoma cells. Methods: MTT assay, cell morphology, agarose gel electrophoresis, and flow cytometry were used to examine taxol-induced apoptosis and growth inhibition in human esophageal carcinoma cell line Ecal09. Results: Taxol was cytotoxic to human esophageal carcinoma cells in a time-dependent manner and dose-dependent manner with a given range. The minimal effective concentration was 6 nmol/L. After treated with taxol, cells at...

To study taxo1-induced apoptosis and growth inhibition in human esophageal carcinoma cells. Methods: MTT assay, cell morphology, agarose gel electrophoresis, and flow cytometry were used to examine taxol-induced apoptosis and growth inhibition in human esophageal carcinoma cell line Ecal09. Results: Taxol was cytotoxic to human esophageal carcinoma cells in a time-dependent manner and dose-dependent manner with a given range. The minimal effective concentration was 6 nmol/L. After treated with taxol, cells at interphase and mitosis underwent apoptosis with typi-cal morphological feature and characteristic apoptotic feature at mitosis respectively. When cells were treated with taxol for 48 h,DNA ladder with agarose gel electrophoresis was observed, and the intensity of DNA ladder was enhanced in a dose- and time-dependent pattern. DNA fragment appeared in cells treated with 3 nmol/L taxol. Apoptotic program of cells treated with taxol can be initiated in short time. Sustained acting was unnecessary. DNA histograms by flow cy-tometric analysis displayed distinct apoptotic peak. Conclusion: The cytotoxic effect of taxol on human esophageal carcinoma cells resulted from taxol-induced apoptosis. This ability of inducing apoptosis may contribute to the clinical effect of taxol. Our results indicate that taxol has great potential for the treatment of esophageal carcinoma.

目的:研究紫杉醇对食管瘤细胞生长抑制及诱导凋亡的作用.方法;应用MTT分析法、形态学观察、琼脂糖凝胶电泳、流式细胞术等方法对紫杉醇诱导的食管癌细胞系Eca109进行了检测和观察.结果:紫杉醉在6 nmol.L浓度时对食管癌细胞就有显著的生长抑制作用,并且有时间依赖关系及一定范围的剂量依赖关系.凋亡可发生在细胞间期和分裂期,分别具有典型的凋亡形态特征及分裂期特有的凋亡特征.琼脂糖凝胶电泳显示紫杉醇作用48h便可出现DNA梯带,并呈时间剂量性增强.最低3 nmol/L浓度可见DNA梯带.紫杉醇可很快启动细胞凋亡的程序,并不需要持续作用.用流式细胞仪测定细胞周期,可见明显的凋亡峰.结论:紫杉醇对食管瘤细胞的细胞毒作用是诱导其凋亡的结果,这种诱导凋亡的能力是紫杉醇疗效的基础,因而在对食管瘤的治疗中紫杉醇有着巨大的潜力.

pDOR p21sense and pDOR p 21 antisense retroviral expression vectors were constructed and successfully transfected by Lipofectin into normal human diploid fibroblasts(2BS line), resulting in 2BS p 21s and 2BS p 21a cell lines, respectively. Southern blot analysis verified that the exogenous p 21 WAF1 cDNA were integrated into genomic DNA. Compared with the cells transfected with pDOR neo empty vector, p 21 WAF1 mRNA expression increased in 2BS p 21s cells, which were less sensitive...

pDOR p21sense and pDOR p 21 antisense retroviral expression vectors were constructed and successfully transfected by Lipofectin into normal human diploid fibroblasts(2BS line), resulting in 2BS p 21s and 2BS p 21a cell lines, respectively. Southern blot analysis verified that the exogenous p 21 WAF1 cDNA were integrated into genomic DNA. Compared with the cells transfected with pDOR neo empty vector, p 21 WAF1 mRNA expression increased in 2BS p 21s cells, which were less sensitive to apoptosis induced by NaBu, and showed higher cell viability, delayed appearance of DNA ladder, and less area of apoptosis peak. On the other hand, p 21 WAF1 mRNA expression decreased in 2BS p 21a cells, which were more sensitive to apoptosis induced by NaBu. These results indicated that the expression amount of p21 WAF1 in 2BS cells was negatively related with its susceptibility to apoptosis induced by NaBu.

构建了有义和反义p21WAF1 逆转录病毒表达载体, 分别经脂质体包裹后转染人胚肺二倍体成纤维细胞(2BS)。Southern 印迹杂交证实转染细胞中外源p21 WAF1cDNA 已整合入基因组中。与空载体转染细胞相比, 有义转染细胞的p21WAF1 m RNA 表达上升; 细胞增殖速度明显减慢; 对丁酸钠诱导凋亡的敏感性降低, 表现在细胞存活率升高, 核DNA 梯状断裂片段出现的时间滞后, 断裂片段浓度下降, 流式细胞计检测的凋亡峰面积缩小。而反义转染细胞的p21WAF1 m RNA表达下降; 细胞增殖速度较快; 对丁酸钠诱导凋亡的敏感性上升, 有关表现与有义转染细胞相反。说明2BS细胞内p21WAF1 的表达量与其被丁酸钠诱导凋亡的能力呈负相关。

Objective To study the effect of Fas gene transfection on bladder cancer cells. Methods Fas cDNA were transduced into bladder cancer cells EJ by DOTAP liposomal transfection regeant. Northern blot,in situ hybrization and flow cytometric evaluation were used to confirm the Fas mRNA and protein expression. The apoptosis and proliferation of EJ cells pre and posttransfection induced by cisplatin were analysed by flow cytometry、DNA ladder and MTT methods. Results Transfection of Fas gene can significantly upregulate...

Objective To study the effect of Fas gene transfection on bladder cancer cells. Methods Fas cDNA were transduced into bladder cancer cells EJ by DOTAP liposomal transfection regeant. Northern blot,in situ hybrization and flow cytometric evaluation were used to confirm the Fas mRNA and protein expression. The apoptosis and proliferation of EJ cells pre and posttransfection induced by cisplatin were analysed by flow cytometry、DNA ladder and MTT methods. Results Transfection of Fas gene can significantly upregulate the expression of Fas in human bladder cancer EJ cells. Apoptosis and decrease of proliferation were easily induced by cisplatin in the transfected EJ cells. Conclusions Fas system might involve in the development and progression of urogenital malignant tumors. Transfection of Fas gene by lipofectin can significantly upregulate the Fas expression in target cell. The synergistic cytotoxic effect obtainted in EJ cells suggested that combined use of Fas gene transfection and cisplatin may help in the treatment of cisplatin resistant bladder cancer.

目的 探讨Fas基因转染对膀胱癌细胞的作用。 方法 将人FascDNA通过脂质体导入膀胱癌细胞EJ中 ,并利用Northernblot、原位杂交、流式细胞术检测细胞的FasmRNA和分子表达。用流式细胞仪、DNA梯形带和MTT法分析顺铂对转染前后的EJ细胞抑制增殖和诱导凋亡的能力。 结果 Fas基因转染可明显增强膀胱癌细胞EJ的Fas表达 ,顺铂对转染后的EJ细胞抑制细胞增殖和诱导凋亡的能力明显增强。 结论 转染的Fas基因可显著上调膀胱癌细胞EJ的Fas表达 ,促进细胞凋亡 ,联合应用Fas基因转染和顺铂可获得协同的细胞毒作用

 
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