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原始荧光信号
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  fluorescence signal
     Fluorescence signal was detected by Boxcar. Three life-time components (320 fs,4.0 ps and 20.0 ps) were obtained by multi-exponential curve model and nonlinear least-square fitting method.
     原始荧光信号由Boxcar采集 ,通过建立多指数模型 ,用非线性最小二乘法拟合 ,得到了激发能在LHCⅡ中传递的时间常数分别为 :32 0fs、4.0ps和 2 0 .0ps。
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  相似匹配句对
     Fast Simulation of SAR Raw Signal
     SAR原始信号的快速仿真
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     Biochip and fluorescence detection
     生物芯片及其荧光信号检测
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     Raw Signal Simulation of Synthetic Aperture Radar
     SAR原始信号模拟研究
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     Design of a Weak Fluorescence Signal Detection Circuit
     荧光微弱信号检测电路设计
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     New method to realize remote signal measure
     信号遥测方法
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  fluorescence signal
The microwave field induced magnetic dipole transitions between the magnetic sublevels of the 5S (F=2) and 5S (F=3) states, resulting in a change in the fluorescence signal.
      
The second type of energy trap gives the dominant contribution to the fluorescence signal at a registration wavelength having the oblique geometry or orthogonal direction of the transition dipole moments of the interacting pyroPheo molecules.
      
The resulting fluorescence signal shows a linear response to the quantity of solute present over 2 to 3 orders of magnitude (correlation coefficients: 0.990-0.98).
      
It was necessary to use acetic acid in the mobile phase to achieve good separation, but this led to fluorescence signal suppression, because puerarin and daidzein have native fluorescence at pH 8.0-9.0.
      
Results: After gene transfection and ping-pong transduction, amphotropic producer line Am12/LGSN was generated with a stable green fluorescence signal readily detectable by FCM in up to 97% of examined cells.
      
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Fluorescence properties of light-harvesting complex (LHCⅡ) in photosystem Ⅱ isolated from spinach (Spinacia oleraceaL.) was investigated with the time-resolved fluorescence spectroscopic technique. The sample was excited by double frequency Ti:Al 2O 3 laser with 400 nm wavelength and 120 fs width. Fluorescence signal was detected by Boxcar. Three life-time components (320 fs,4.0 ps and 20.0 ps) were obtained by multi-exponential curve model and nonlinear least-square fitting method. Three fluorescence peaks...

Fluorescence properties of light-harvesting complex (LHCⅡ) in photosystem Ⅱ isolated from spinach (Spinacia oleraceaL.) was investigated with the time-resolved fluorescence spectroscopic technique. The sample was excited by double frequency Ti:Al 2O 3 laser with 400 nm wavelength and 120 fs width. Fluorescence signal was detected by Boxcar. Three life-time components (320 fs,4.0 ps and 20.0 ps) were obtained by multi-exponential curve model and nonlinear least-square fitting method. Three fluorescence peaks (652 nm, 672 nm, 691 nm) were obtained by Global analysis and Gauss curve fitting. These components were analyzed according to the structural and fluorescence spectroscopic property of LHCⅡ. The fluorescence ratio of each component to that of all the component was 3.4%, 50% and 46.6% respectively. The possible model of energy transfer in LHCⅡ was discussed.

采用时间分辨荧光光谱技术研究了菠菜 (SpinaciaoleraceaL .)叶绿体中捕光色素复合体 (LHCⅡ )荧光的时间特性和光谱特性。用脉宽为 12 0fs、波长为 40 0nm的倍频钛宝石激光激发LHCⅡ样品 ;原始荧光信号由Boxcar采集 ,通过建立多指数模型 ,用非线性最小二乘法拟合 ,得到了激发能在LHCⅡ中传递的时间常数分别为 :32 0fs、4.0ps和 2 0 .0ps。相对应的各组分荧光占总荧光的百分比分别为 :3.4%、5 0 .0 %和 46 .6 %。经全局分析 ,解得荧光强度随波长变化曲线 ;用高斯 3峰解叠得到荧光光谱的峰值波长分别为 :6 5 2nm、6 72nm和 6 91nm。通过分析得出了时间常数与LHCⅡ中各色素成分之间的对应关系 ,并给出了可能的能量传递模型

 
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