The number of migrating cells was about 4 times higher in the proliferative EC group(n=6,P<0.05)than in the control group,while tha t in the confluent EC group was only half of the number of the control(n= 6,P<0.05). The proliferative ECs inhibited and confluent EC promoted signi ficantly the expression of α-SM-actin mRNA.
Tumstatin and tumstatin's 74-98aa peptide have been shown inhibiting activation of FAK,PI3-kinase, protien kinase B (PKB/Akt), and mammalian target of rapamycin (mTOR) in endothelial cells. Furthermore, those prevents the dissociation of eukaryotic initiation factor 4E protien (eIF4E) from 4E-binding protien 1 , leading to the inhibition of cap-dependent translation.
Results:the effect for normal EVC304 was not powerful of HSYA, But the remarkably inhibit the growth of endothelial cell with the stimulus of tumor cell conditioned medium of HSYA ( P < 0.001 compared with the tumor group). Were observed in time - dependent and dosage - inverse ratio manner.