AIM:To evaluate the diagnostic value of four specific antigens from cDNA of Cysticercus cellulosae . cC1,cC2,and cP1 and cH1 ( 28 kDa, 18 kDa, 14 kDa and 34 kDa ),mixing in equal proportions for the diagnosis of cysticercosis.
Results: The diagnostic sensibility of spiral CT in non-calcified costal cartilage of human individual is 93.54%, accurate rate is 93.93%, positive predictive value is 93.54%, false positive rate 3%, false negative rate is 3%.
In this paper, the plate agglutination test was applied to examine the antibody against Trichinosis in human and swine, and the result showed that this method for diagnosis of Trichinosis to handy in manipulation, acute in reaction,speed and accurate in result.
A kit of rapid ELISA for diagnosis of trichinosis has been developed and used to determine serum samples from 107 cases with trichinosis in paralell with the routine ELISA with the result that positive rate was 99. 07% (106/107) for the former and 97. 19% (104/107) for the latter. They were also used to determine serum samples from 10 pigs,4 dogs and 5 rabbits infected with trichina with positive rate 100% for pigs and dogs and 80% for rabbits. No significant differences were found between the two methods o...
Using self-construted PBC4 plasmid.224bp Brucella abortus 544A fragment is got by Kpnl and BamHl.Then the probe is prco cessed by labelling digitoxin post purification dot blot indicated that the probe’s sensitivity is 5.0pg.ninteen types of Brucella show strong bloting signal .but five species of Gram negative bacteria and human WBC DNA do not show any hybridization signal.The tech nigue will be a effective method for laboratory cliagnosis of human or animal Brucella.