In this regard, nude mice model of human hepatocellular carcinoma (LTNM1、LTNM2、LTNM3、LTNM4 ), nude rat model of human hepatocellular carcinoma (LTNR1、LTNR2), nude mice model of highly metastatic human hepatocellular carcinoma (LCI-D20), and highly metastatic human hepatocellular carcinoma cell line MHCC97 have been established and extensively studied.
Objective To study the effect of two cAMP analogs,8-Cl-cAMP(with site-selective for PKAⅡ) and DBcAMP(non-site-selective) on growth and differentiation of highly metastatic human lung cancer cells PGCC.
Methods: Our study was conducted on a highly metastatic human breast cancer cell line MDA-MB-231. Taking benzyl-α-GalNAc(a glycosilation inhinbitor) and RPMI-1640 group as positive and negative control respectively.
Methods: Micropipette aspiration technique was used to investigate quantitatively the adhesion force (Fa) and the relative adhesion stress (S1) between high metastatic human lung giant cell carcinoma (PG) cells or low metastatic lung adenocarcinoma (PAa) cells and human umbilical vein endothelial cells (HUVEC).
[Purpose]To explore the effects of retinoic acid(RA),glycyrrhizin(GL),18β-glycyrrhetinic acid(GA),the combination of RA and GL,and the combination of RA and GA on proliferation inhibition and anti-invasion in highly metastasis human lung can-cer cells(PGCL3).
We also used flow cytometer observed the expression of the thrombocytic immune related antigen CD42a, TSP and CD9 on PG and PAa, and the alter of the expression of CD42a,TSP and CD9 when the two cell lines were incubated with different level Elemene.
Objective: To study the gene expression of high metastatic human ovarian carcinoma cell line (HO-8910PM) and to screen for novel metastasis- associated genes by cDNA microarray.
We examined the expression of Gal-4 in primary and metastatic human ileal carcinoid tumors as well as in carcinoid tumors of the stomach, lung, and rectum.
HT1080 (metastatic human fibrosarcoma) and WI38 VA13 (virus-transformed human fibroblasts) were grown on substrates of collagen, collagen/hyaluronic acid or chondroitin sulphate and reconstituted basement membrane (Matrigel).
The HPRT deficiency had been induced with MNNG (N-methyl-N'-nitro N-nitrosoguanidine) in CHO-k1 cells and the cell clones were selected in a culture medium containing 6-thio-guanine (6-TG) .Although the cells were cultured for over 15 generations, the phenotype of HPRT- was stable.By the calcium phosphate coprecipitation and the electropration methods, the HeLa S3 DNA was transfered into HPRT gene deficient CHO cells. The' results indicated that the HPRT deficiency in the recipient cells was partially corre...
A highly metastatic giant cell carcinoma (PG) and a poorly metastatic adenocarcinoma (PAa) were orthotopically implanted into nude mice, and the take rates were 74% for PG and 56% for PAa. Invasive tumors located in hilar region or peripheral part of lung, or spread diffusely, which were quite similar to three macroscopic types of primary lung carcinomas and different from well-circumscribed masses of ectopically implanted tumors. It is interesting to point out that the metastatic incidence of PAa cells in ...
re-treatment of metastatic human lung cancer cell subline (PGCL3) with all trans retinoic acid(RA)resulted in inhibition of cell growth in vitro and invasion through the reconstituted basement membrane. RA was also noticed to inhibit the experimental metastatic ability of PGCL 3. Data showed that 5/6,3/6 and 2/6 of the nude mice developed lung colonization in the control, the 5μmol/L and the 10 μmol/L RA treated PGCL3 cells respectively.Data from DNA-RNA dot blot hybridization further showed that the 10 μmo...