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子宫颈损伤
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  uterine cervical lesions
     Conclusion Telomerase activity may play an important role during cervical carcinogenesis,and telomerase activity is strongly associated with HPV infection and oncogene in uterine cervical lesions.
     结论端粒酶活性在宫颈癌发生中可能起到重要作用,在子宫颈损伤中端粒酶激活与HPV感染及其致癌基因的表达密切相关;
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     Conclusion:Telomerase activity may play an important role in cervical carcinogenesis,and telomerase activity is strongly associated with HPV infection and oncogene in uterine cervical lesions.
     结论:端粒酶活性在宫颈癌发生中可能起到重要作用,在子宫颈损伤中端粒酶激活与HPV感染及其致癌基因的表达密切相关;
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  相似匹配句对
     Duodenal injuries
     十二指肠损伤
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     An Analysis of Rectal Injury Following Afterloading Radiotherapy in 158 Cases with Cervical Cancer
     158例子宫颈癌放疗后放射性直肠损伤分析
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     Histopathological Observation of Cervical Specimens After Thermal Damage from the Loop Electrosurgical Excision Procedure
     子宫颈电圈环切术标本的热损伤及组织形态学改变
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     Radiation induced lung injury
     放射性肺损伤
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     Freezing Treatment for Cervix Erosion
     冷冻治疗子宫颈糜烂
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  uterine cervical lesions
The typical MR imaging findings of uterine cervical lesions correspond to the histopathologic features.
      


Objective To study the relationship between telomerase activity,HPV 16/18 infection and its carcinogenic gene and cervical cancer.Methods 30 cases infiltrating cervical cancer,58 cases of cervical intraepithelial neoplasia (20 cases of CINⅢ,20 cases of CINⅡ,18 cases of CINⅠ)and 16 cases of normal control cervical fresh tissue were measured for telomerase activity using TRAP-PCR,for HPV 16/18 type DNA using FQ-PCR and for oncogene E6,E7 in the tissue of HPV 16/18-positive using PCR.Results We detected strong...

Objective To study the relationship between telomerase activity,HPV 16/18 infection and its carcinogenic gene and cervical cancer.Methods 30 cases infiltrating cervical cancer,58 cases of cervical intraepithelial neoplasia (20 cases of CINⅢ,20 cases of CINⅡ,18 cases of CINⅠ)and 16 cases of normal control cervical fresh tissue were measured for telomerase activity using TRAP-PCR,for HPV 16/18 type DNA using FQ-PCR and for oncogene E6,E7 in the tissue of HPV 16/18-positive using PCR.Results We detected strong telomerase activity in 22 cases of cervical cancer(73.33%)(among 15 cases HPV16/18+, E6 and E7 were positive in 7 cases) and telomerase was negative in 8 cases (1 case HPV16/18+ without E6,E7);telomerase was positive in 13 cases CINⅢ (46.4%),(among 6 cases HPV16/18+,E6 and E7 were positive in 2 cases) and telomerase was negative in 15 cases (none of HPV16/18+);telomerase was positive in 6 cases CINⅡ (25.00%),(among 2 cases HPV16/18+,E6 and E7 were positive in 1 cases) and telomerase was negative in 18 cases (none of HPV16/18+ );telomerase was positive in 2 cases CINⅠ (10.00%),(2 cases HPV16/18+ without E6,E7) and telomerase was negative in 16 cases (none of HPV16/18+);only 1 of 16 normal control tissue was positive of telomerase activity with HPV 16/18 + and without E6,E7.Expression of telomerase activity (P<0.01),HPV 16/18 infection (P<0.01)and expression of HPV 16/18 oncogene(P<0.01) were significantly greater in cervical cancer and precancerous lesions than in benign lesions and normal control.Conclusion Telomerase activity may play an important role during cervical carcinogenesis,and telomerase activity is strongly associated with HPV infection and oncogene in uterine cervical lesions.It is signality for discussing cervical disease advance and cervical carcinogenesis.

目的研究端粒酶活性、人乳头瘤病毒16/18(HPV16/18)感染及致癌基因与宫颈癌的相关性。方法对30例浸润型宫颈癌、58例宫颈内瘤形成(CINⅢ20例、CINⅡ20例、CINⅠ18例)及16例正常女性的宫颈新鲜组织,采用端粒酶重复序列扩增法(TRAP-PCR)检测端粒酶活性,用荧光定量多聚酶链式反应(FQ-PCR)检测HPV16/18DNA,用PCR方法对HPV16/18DNA阳性组织作HPV16型致癌基因E6、E7检测。结果宫颈癌组中端粒酶阳性22例(73.33%)(HPV16/18+15例,7例有E6、E7表达),端粒酶阴性8例(HPV16/18+1例,无E6、E7表达)。CINⅢ级中端粒酶阳性13例(46.4%)(HPV16/18+6例,2例有E6、E7表达),端粒酶阴性15例(无HPV16/18+);CINⅡ级中端粒酶阳性6例(25%)(HPV16/18+2例,1例有E6、E7表达),端粒酶阴性18例(无HPV16/18+);CINⅠ级中端粒酶阳性2例(10.00%)(HPV16/18+2例,无E6、E7表达)。而16例正常宫颈组织仅1例(6.25%)端粒酶活性表达,此例HPV也呈阳性而无E6、E...

目的研究端粒酶活性、人乳头瘤病毒16/18(HPV16/18)感染及致癌基因与宫颈癌的相关性。方法对30例浸润型宫颈癌、58例宫颈内瘤形成(CINⅢ20例、CINⅡ20例、CINⅠ18例)及16例正常女性的宫颈新鲜组织,采用端粒酶重复序列扩增法(TRAP-PCR)检测端粒酶活性,用荧光定量多聚酶链式反应(FQ-PCR)检测HPV16/18DNA,用PCR方法对HPV16/18DNA阳性组织作HPV16型致癌基因E6、E7检测。结果宫颈癌组中端粒酶阳性22例(73.33%)(HPV16/18+15例,7例有E6、E7表达),端粒酶阴性8例(HPV16/18+1例,无E6、E7表达)。CINⅢ级中端粒酶阳性13例(46.4%)(HPV16/18+6例,2例有E6、E7表达),端粒酶阴性15例(无HPV16/18+);CINⅡ级中端粒酶阳性6例(25%)(HPV16/18+2例,1例有E6、E7表达),端粒酶阴性18例(无HPV16/18+);CINⅠ级中端粒酶阳性2例(10.00%)(HPV16/18+2例,无E6、E7表达)。而16例正常宫颈组织仅1例(6.25%)端粒酶活性表达,此例HPV也呈阳性而无E6、E7表达。宫颈癌和癌前病变(CINⅢ)组织端粒酶活性表达频率(P<0.01)和HPV16/18感染率(P<0.01)及其致癌基因表达(P<0.01)均显著高于良性损伤(CINⅠ和CINⅡ)和正常对照。结论端粒酶活性在宫颈癌发生中可能起到重要作用,在子宫颈损伤中端粒酶激活与HPV感染及其致癌基因的表达密切相关;对于探讨宫颈病变的进展和宫颈癌的发生具有重要意义。

Objective:To study the relationship among telomerase activity,HPV 16/18 infection and its carcinogenic gene and cervical cancer.Methods:40 cases of infiltrating cervical cancer,110 cases of cervical intraepithelial neoplasia(32 cases CINⅢ,40 cases CINⅡ,38 cases CINⅠ) and 30 cases of normal control cervical fresh tissue were measured for telomerase activity using TRAP-PCR,for HPV 16/18 type DNA using FQ-PCR and for oncogene E6,E7 in the tissue of HPV 16/18-positive using PCR.Results:The strong telomerase activity...

Objective:To study the relationship among telomerase activity,HPV 16/18 infection and its carcinogenic gene and cervical cancer.Methods:40 cases of infiltrating cervical cancer,110 cases of cervical intraepithelial neoplasia(32 cases CINⅢ,40 cases CINⅡ,38 cases CINⅠ) and 30 cases of normal control cervical fresh tissue were measured for telomerase activity using TRAP-PCR,for HPV 16/18 type DNA using FQ-PCR and for oncogene E6,E7 in the tissue of HPV 16/18-positive using PCR.Results:The strong telomerase activity in 40 cervical cancer(95%)(among 39 cases HPV16/18+,E6,E7 was positive in 17 cases) and telomerase was negative in 8 cases(1 case HPV16/18+ without E6,E7);Telomerase was positive in 28 cases CINIII(46.4%)(among 39 cases HPV16/18+,E6,E7 was positive in 22 cases) and telomerase was negative in 15 cases(none of HPV16/18+);Telomerase was positive in l2 cases CINⅡ(25.00%)(among 16 cases HPV16/18+,E6,E7 was positive in 4 cases) and telomerase was negative in 18 cases(none of HPV16/18+);Telomerase was positive in 3 cases CIN Ⅰ(10.00%),(0 case HPV16/18+ without E6,E7) and telomerase was negative in 16 cases(none of HPV16/18+);Only 1 of 16 normal control tissues was positive of telomerase activity with HPV 16/18 + and without E6,E7.Expression of telomerase activity(P<0.01),HPV 16/18 infection(P<0.01) and expression of HPV 16/18 oncogene(P<0.01) were significantly greater in cervical cancer and precancerous lesions than those in benign lesions and normal control.Conclusion:Telomerase activity may play an important role in cervical carcinogenesis,and telomerase activity is strongly associated with HPV infection and oncogene in uterine cervical lesions.It is significant for discussing cervical disease advance and cervical carcinogenesis.

目的:探讨端粒酶活性、HPV 16/18感染及致癌基因与宫颈癌的相关性。方法:对40例浸润型宫颈癌,110例宫颈上皮内瘤变(C INⅢ32例、C INⅡ40例、C INⅠ38例)及30例正常者的宫颈新鲜组织,用TRAP—PCR检测端粒酶活性、用FQ—PCR检测HPV l6/18DNA、用PCR方法对HPV 16/18 DNA阳性组织作HPV 16型致癌基因E 6、E 7检测。结果:宫颈癌组中端粒酶阳性38例(95.00%)(HPV 16/18+39例,17例有E 6、E 7表达),端粒酶阴性2例(HPV 16/18+1例,无E 6、E 7表达):C INⅢ级中端粒酶阳性28例(87.5%)(HPV 16/18+30例,22例有E 6、E 7表达),端粒酶阴性4例(2例HPV16/18+);C INⅡ级中端粒酶阳性12例(30%)(HPV 16/18+16例,4例有E 6、E 7表达),端粒酶阴性28例(4例HPV 16/18+);C INⅠ级中端粒酶阳性3例(7.89%)(HPV 16/18+5例,无E 6、E 7表达):而30例正常宫颈组织仅有2例(6.67%)端粒酶活性表达,1例HPV也为阳性而无E 6、...

目的:探讨端粒酶活性、HPV 16/18感染及致癌基因与宫颈癌的相关性。方法:对40例浸润型宫颈癌,110例宫颈上皮内瘤变(C INⅢ32例、C INⅡ40例、C INⅠ38例)及30例正常者的宫颈新鲜组织,用TRAP—PCR检测端粒酶活性、用FQ—PCR检测HPV l6/18DNA、用PCR方法对HPV 16/18 DNA阳性组织作HPV 16型致癌基因E 6、E 7检测。结果:宫颈癌组中端粒酶阳性38例(95.00%)(HPV 16/18+39例,17例有E 6、E 7表达),端粒酶阴性2例(HPV 16/18+1例,无E 6、E 7表达):C INⅢ级中端粒酶阳性28例(87.5%)(HPV 16/18+30例,22例有E 6、E 7表达),端粒酶阴性4例(2例HPV16/18+);C INⅡ级中端粒酶阳性12例(30%)(HPV 16/18+16例,4例有E 6、E 7表达),端粒酶阴性28例(4例HPV 16/18+);C INⅠ级中端粒酶阳性3例(7.89%)(HPV 16/18+5例,无E 6、E 7表达):而30例正常宫颈组织仅有2例(6.67%)端粒酶活性表达,1例HPV也为阳性而无E 6、E 7表达。宫颈癌和癌前病变(C INⅢ)组织端粒酶活性表达频率(P<0.01)和HPV 16/18感染率(P<0.01)及其致癌基因表达(P<0.01)显著高于良性病变(C INⅠ和C INⅡ)和正常对照。结论:端粒酶活性在宫颈癌发生中可能起到重要作用,在子宫颈损伤中端粒酶激活与HPV感染及其致癌基因的表达密切相关;对于探讨宫颈病变的进展和宫颈癌的发生发展具有重要意义。

>=In the obstetric diseases, Dairy cow's injury of birth canal usually shows injury of cervix and vaginal. When occurring the injury of birth canal, which can lead to abscess of vagina and the hyperplasia of granulation tissue, Leading Dairy cow's death. Treating injury of birth canal by integrating Chinese-western therapy, the cure rate can reach to 75~90%.

奶牛产道损伤在产科病中,多见子宫颈损伤和阴道损伤。一旦奶牛发生产道损伤,如不及时正确的治疗,可继发阴道脓肿或肉芽增生,也可引起奶牛不孕、甚至死亡。应用中西结合的治疗方法,对产道损伤的不同病症分别进行治疗试验,治愈率可达75—90%。

 
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