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trishcl
相关语句
  tris-hcl
     The satisfactory condition of enzyme catalytic reaction was given: 0. 1 M, pH 7.5 Tris-Hcl buffer, reacted 10 min. in 37°C.
     确定了该酶催化反应的最佳条件:0.01M,pH 7.5 Tris—HCl缓冲液,37℃反应10分钟.
短句来源
     The standard lysozyme was treated into 100 mmol/L Tris-HCL solution containing 6 mol/L guanidine chloride and 2% SDS surfactant with nitrocellubse membrane as stationary phase.
     采用硝酸纤维素膜作为固相载体,将标准蛋白质溶菌酶制成含6 mol/L盐酸胍变性剂、2%SDS表面活性剂的100 mmol/L Tris—HCL溶液进行质谱测定。
短句来源
     The crude product of HLC Ⅰ flowed through the DE-52 ion exchange pole,then the Tris-HCl buffer solution eluate the HLC Ⅰ .
     经实验确定离子交换柱层析的实验条件为:类人胶原蛋白Ⅰ起始进样浓度为3g/L,以pH7.0、浓度为0.020mol/L NaCl含量为0.15mol/L的Tris—HCl溶液配制,然后在DEAE-52上以3.0mL/min过柱吸附;
短句来源
     Transglutaminase can improve properties of soybean protein film. The optimum compositions of film solution are determined as follows: soybean protein 5% , glycerol 3% and enzyme 0.2 % are solved in Tris - HCl buffer solution at pH8. When the concentration of the enzyme is 0.2% in the film - making solution, the index of comprehensive properties such as antiextensibility ductility water proofness and oil proofness, are better than other enzyme concentrartion.
     转谷氨酰胺酶能改善大豆蛋白膜性能,实验确定最佳成膜液组分为大豆蛋白5%,甘油3.0%,酶0.2%,并溶解在pH8的Tris—HCl缓冲溶液中。 0.2%酶浓度的成膜液所成膜的综合性能指标如抗拉伸度、延展性、阻水性和阻油性等均优于其它酶浓度成膜液。
短句来源
  “tris—hcl”译为未确定词的双语例句
     The heteroprotein was absorbed by DE52 batch chromatography. The supernatant was collected, and flowed through the Sephadex G-100 column for Gel Filtration Chromatography. And 20mmol/LTris-HCl (pH 7.5 20mmol/L sodium chloride) buffer was used as eluent.
     采用批量层析的操作方法在pH7.5条件下用DE52树脂吸附杂蛋白,收集吸附后的上清液,经Sephadex G—100凝胶过滤层析柱,洗脱液为20mmol/L Tris—HCl(pH7.5含20mmol/L NaCl)缓冲液。
短句来源
     The Merozoites of precocious and attenuated strains of E. acervulina were puritied via DEAE—52chramat-ographic column (φ=1.5×5cm) in. PH7. 6 Tris—Hcl (Velocity of flow=0.5~1.5ml/min) with 50.49%~56.2%.
     自实验感染堆型艾美耳球早熟减毒株卵囊的雏鸡小肠内容物中得到裂殖子悬液,在直径1.5cm、高5cm的DEAE52层析柱中,用PH7.6Tris—Hcl、以0.5~1.5ml/分钟流速洗脱纯化,得到纯净的裂殖子,回收率为50.49%~56.2%。
短句来源
     The purity of human-like collagen could be 97.6%, and the total recovery was 63.0%. This is so-called the combination of Ion Exchange batch Chromatography & Gel Filtration Chromatography.
     收集吸附后的上清液,经SephadexG—100凝胶过滤层析柱,洗脱液为20mmol/L Tris—HCl(pH7.5含20mmol/LNaCl)缓冲液。 可获得纯度为97.6%的类人胶原蛋白Ⅰ,总回收率为63.0%。
短句来源
     OlM Tris-HC1 buffer, pH 8.0 for at least one month -without significant loss of activity. At 4X). however, more than 76% of the activity was lost within 30 days.
     在分离纯化过程中未发现蚕体内有该酶的同功酶存在,纯化后的酶溶解于pH8.0、0.01M的Tris—HCl缓冲掖中,-30℃经过一个月没有发现明显的失活现象,但在4℃中一个月后丧失活性76%。
短句来源
     By treatment of cell engineering technology 240 units per ml of elastase are produced by Flavobacterium sp. No. 122 which are about 1. 5 times higher than that produced by the original strain.
     经过细胞工程技术处理后,黄杆菌属中的F-122菌在摇瓶发酵中的产酶能力达到240u/ml,比出发菌株提高了1.5倍。 该酶在Tris—HCl缓冲液中最适pH为8.0,最适酶解温度43℃。
短句来源
  相似匹配句对
     Determination can be made at pH 7.0,where the native structure of DNA is not disrupted.
     L-1,Tris-HCl缓冲溶液,pH 7.0。
短句来源
     L-1 of HC1. The mechanics of hydrolysis with microwaves were deeply discussed.
     L~(-1)HCl
短句来源
     temocapril - HC1;
     temocapril-HCl
短句来源
     and the substrate buffer solution may be prepared by 0.05 tool/L pH 7.6 Tris-HCl.
     底物缓冲液为0.05mol/LpH7.6Tris-HCl
短句来源
     The satisfactory condition of enzyme catalytic reaction was given: 0. 1 M, pH 7.5 Tris-Hcl buffer, reacted 10 min. in 37°C.
     确定了该酶催化反应的最佳条件:0.01M,pH 7.5 TrisHCl缓冲液,37℃反应10分钟.
短句来源
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  trishcl
GroEL labeled in 50mM TrisHCl, pH 7.8, incorporated ~0.3 labels each on C138 and C458.
      
The emf was recorded for equimolal bis-tris/bis- trisHCl buffer solutions from 5 to 125°C at approximately 25°C intervals, and at nine ionic strengths from 0.05 to 5.0m (NaCl).
      
After 12 h at 55 C, the extract was purified using TrisHCl-saturated buffered phenol and a chloroform/isoamyl solution.
      
All Abs were diluted to the appropriate concentration in TrisHCl pH 7.5 with 0.1% Tween-20 and 0.1% sodium azide.
      
Column fractions were immediately neutralised and dialysed against 10 mM TrisHCl, pH 7.5.
      
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The studies of affectors affecting RAPD were carried out by the 12 species of CLanatus as template DNAThe results showed that the purification of template DNA,the concentrations of template DNA,Taq polymerase,random primer,Mg2+and the PCR programe played an important role in RAPD analysisBased on the researches above,a technical system for RAPD analysis in species of Clanatus was established:in 10 L reaction solution,containing 10 ng/10 L template DNA,05 mol/L random primer,50 mmol/L TrisHCl(pH83),500...

The studies of affectors affecting RAPD were carried out by the 12 species of CLanatus as template DNAThe results showed that the purification of template DNA,the concentrations of template DNA,Taq polymerase,random primer,Mg2+and the PCR programe played an important role in RAPD analysisBased on the researches above,a technical system for RAPD analysis in species of Clanatus was established:in 10 L reaction solution,containing 10 ng/10 L template DNA,05 mol/L random primer,50 mmol/L TrisHCl(pH83),500 g/mL BSA,25 mmol/L MgCl2,06 U/10 L Taq DNA,200 mol/L dNTPs

以西瓜种内的12个品种(系)为模板DNA,研究了影响RAPD扩增效果的因素。结果表明,模板DNA、Taq酶、随机引物、Mg2+浓度以及PCR反应程序在RAPD分析中都具有重要的作用。根据以上因子的研究结果,建立了适合西瓜种植物的RAPD分析的技术体系:10μL反应液中,模板10ng/10μL,引物05μmol/L,TrisHCl(pH83)50mmol/L,BSA500μg/mL,MgCl225mmol/L,TaqDNA06U/10μL,dNTPs200μmol/L,10%蔗糖400和1mmol/L甲酚红。

Contraceptive levonorgestrel (LNG) was coupled to polyα、β(3hydroxypropyl)DLaspartamide,a biodegradable and biocompatible material.The drug loaded in the polymer was about 27.0% (w/w). The polymer conjugate was characterized by differential scanning calorimertry (DSC) and fourier transform infrared (FTIR). The in vitro release of levonorgestrel from the biocompatible polymer conjugate was studied in TrisHCl (0.1 mol/L) and trypsin. Levonorgertrel was measured by HPLC. The data indicate that this levonorgestrel...

Contraceptive levonorgestrel (LNG) was coupled to polyα、β(3hydroxypropyl)DLaspartamide,a biodegradable and biocompatible material.The drug loaded in the polymer was about 27.0% (w/w). The polymer conjugate was characterized by differential scanning calorimertry (DSC) and fourier transform infrared (FTIR). The in vitro release of levonorgestrel from the biocompatible polymer conjugate was studied in TrisHCl (0.1 mol/L) and trypsin. Levonorgertrel was measured by HPLC. The data indicate that this levonorgestrel polymer conjugate can be released in these two systems but faster in trypsin.$

具有临床意义的甾体类避孕药左旋 1 8甲基炔诺酮 (L NG)键合于生物相容性聚氨基酸材料 :α、β 聚 (3羟丙基 ) DL 天冬酰胺上 ,制成长效控释药物。用红外光谱法、差热分析等分别对材料及键合药物进行了表征 ,药物的接入率达 2 7.0 % (w/ w) (n=3) ,共价缩合物以棒状形式释药进行了体外水解和酶解释放试验。结果表明 ,酶解释放比水解释放略快

A proteinase,which has important work on turbot digestive,was purified from turbot(Scophthalmus maximus L.) and its major physical and chemical characteristics were studied and reported in this article.The intestine proteinase extraction was prepared through homogenate and centrifugation by Tris-HCl buffer at low temperature.The supernatant was purified through ammonium sulfate grading precipitation further and the active component was dialyzed and concentrated.Then the condensed proteinase was purified by means...

A proteinase,which has important work on turbot digestive,was purified from turbot(Scophthalmus maximus L.) and its major physical and chemical characteristics were studied and reported in this article.The intestine proteinase extraction was prepared through homogenate and centrifugation by Tris-HCl buffer at low temperature.The supernatant was purified through ammonium sulfate grading precipitation further and the active component was dialyzed and concentrated.Then the condensed proteinase was purified by means of DEAE-Sepharose Fast Flow anion-exchange chromatography with an elution of a linear gradient of 5 mmol·L~(-1) Tris-HCl buffer(pH 8.5) containing 1.0 mol·L~(-1) NaCl.At last,the pure enzyme was obtained through Sephadex G-100 chromatography with an elution of 5mmol·L~(1) TrisHCl buffer(pH 8.5).The purity of the purified intestine proteinase was confirmed by the presence of a single band on SDS-PAGE.The relative molecular mass of this enzyme was determined to be about 58 kD.By using casein as substrate to measure proteinase activity,the characterization of turbot intestine proteinase was made.The enzyme is stable at pH 6.0-11.0 and temperature below 30 ℃.When the temperature rose,there was a rapid decline of its stability.The enzyme showed maximum activity at pH 9.0 and 50 ℃.When the temperature declined,the optimum pH of enzyme showed a trend of moving to alkaline.And when the action time prolonged,the optimum temperature of enzyme showed a trend to decline.Furthermore,the enzyme is activated by Mn~(2+) and inactivated by Cu~(2+),Zn~(2+),Ag~+,Fe~(3+).In further studies,it was inhibited by SH-enzyme inhibitors such as p-Hydroxymercuribenzoate(PCMB) and N-bromosuccinimide(NBS) remarkably,and partially inhibited by tosyl-lysine chloromethyl ketone(TLCK).Using casein as the substrate, the Km value of enzyme is 2.92 g·L~(-1) at pH 9.0,50 ℃.The result showed the enzyme seemed to be a SH-enzyme.

以大菱鲆肠道为材料,对大菱鲆消化生理中起重要作用的肠蛋白酶的分离纯化条件和理化性质进行了研究。经Tris-HCl缓冲液抽提,硫酸铵分级分离,DEAE-Sepharose Fast Flow离子交换层析、Sephadex G-100凝胶过滤层析分离纯化,获得大菱鲆肠蛋白酶Ⅰ的电泳纯制品,并对该酶的性质进行了研究。结果显示:纯酶的分子量约为58kD。纯酶最适反应pH为9.0,最适反应温度50℃;pH稳定范围6.0~11.0,30℃以下酶活性稳定;Mn2+可激活大菱鲆肠蛋白酶Ⅰ,Cu2+、Zn2+、Ag+、Fe3+则对酶活性有抑制作用;巯基蛋白酶抑制剂显著抑制大菱鲆肠蛋白酶Ⅰ的活性,金属蛋白酶抑制剂对大菱鲆肠蛋白酶Ⅰ无影响。50℃、pH9.0条件下以双倒数作图法得大菱鲆肠蛋白酶Ⅰ催化酪蛋白水解的Km值为2.92g.L-1。

 
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