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只balb
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  balb
     Methods 48 male BALb/c mice were divided into 4 groups, according to the n-6/n-3 PUFA ratio of 1, 7.5, 15 and 30, respectively.
     方法48只BALB/c小鼠随机分为4组,即n-6/n-3比值分别为1,7.5,15,30;
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     Fifty BALB/c male mice were divided into five groups, which were immunized intramuscularly with multivalent DNA vaccine PV-IL12-Sj23, plasmid PV-Sj23 expressing Sj23, plasmid PV-IL12 expressing cytokine IL-12, blank plasmid PV and saline, respectively. Each mouse was immunized with 100 μg DNA only once.
     50只BALB/c小鼠分为5组,每组10只,分别注射多价DNA疫苗PV-IL12-Sj23、表达Sj23的质粒PV-Sj23、表达IL-12质粒PV-IL12、空白质粒PV和生理盐水,100μg/只,免疫1次。
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     Methods Each of BALB/c mice was infected with 5×10 5 RBC infected P.
     方法每只BALB/c小鼠接种5×105个感染P.
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     Methods Sixty BALB/c mice were divided randomly into four groups: control group (tap water, NC), iodine excess group (3000μg/L I,EI+),selenium supplement group (200μg/L Se, Se+) and iodine excess plus selenium(3000μg/L+ I 200μg/L Se, EI+Se+) group.
     方法将60只BALB/c小鼠随机分为4组:正常对照组(饮用自来水,NC)、碘过量组(饮水3000μg/LI,EI+)、单独补硒组(饮水200μg/LSe,Se+)和碘过量加硒组(饮水3000μg/LI+200μg/LSe,EI+Se+)。
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     Methods 60 Balb/c mice were divided randomly into four groups:control group,excess iodine group(3?000?μg/L I),supplement selenium group(200?μg/L Se)and excess iodine plus selenium(200?μg/L Se)group.
     方法将60只Balb/c小鼠随机分为4组:正常对照组、高碘组(I 3 000μg/L)、单独补硒组(Se 200μg/L)、高碘(I 3 000μg/L)+Se(200μg/L)组。
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  “只balb”译为未确定词的双语例句
     Both 20 Ba1b/c and C57b1/6 mice were intraperitoneally innoculated with 0.2ml Of CVB_1 10~(-3),10~(-5) and 10~(-7) TCID_(50) for 1~3 weeks separately.
     和不同毒力0.2mlCVB_1 10~(-3),10~(-5),10~(-7)TCID_(50)分别腹腔接种各20只BALB/C和C57BL/6小鼠;
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     Methods The two dosages of matrine (50 mg/kg and 100 mg/kg) combined respectively with 50 mg/kg of 5-fluorouracil (5-FU) were injected intra-abdominally, and 50 mg/kg or 100 mg/kg matrine or 5-FU injection alone groups served as controls.
     方法 将42只Balb/c小鼠分为阴性对照组(12 只)、5- FU组(6 只)、苦参碱A组(50 mg/kg,6只)、苦参碱B组(100 mg/kg,6只)、联合用药A组(苦参碱50 mg/kg+5 -FU,6只)和联合用药B组(苦参碱100 mg/kg+5 -FU,6只)。
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     After 12 and 24 wk of infection, all of 7 infected Babl/c and 6 of 7 the H pylori infected C57BL/6 mice showed the positive results.
     12和24wk后的实验组7只Balb/c 小鼠胃组织细菌培养均为阳性,而7只C57BL/6小鼠中有6只显示阳性结果.
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     B? C and D groups,12 mice of each group.
     把 48只 BAL B/ c小鼠随机分为 A、B、C、D 4组 ,每组 12只。
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     The mice given gene gun inoculation were divided into 3 groups, each group containing 3 mice: groupⅠ as control with 1.5 μg pcDNA3.1;
     经基因枪免疫 9只BALB/C小鼠 ,3组小鼠分别注射 1.5 μgpcDNA3.1、pcDNA3.1 G1、pcDNA3.1 G2 ;
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     10 Balb/c mouse were immunized with subtractive immunization method.
     消减免疫法免疫 10Balb/c小鼠。
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     PPO and PAL could also be triggered by infection with R.
     接种R .
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     Fifteen male BALB/c nude mice were used.
     实验选用BALB/c裸雄性小鼠15
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     Water Pots
     两水桶
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     Bio-cleaning and Inbreding of BALB/c Mice
     BALB/c小鼠的净化
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  balb
TK-/gE-/GP5m+ and TK-/gE-/GP5+ expressing the authentic GP5 protein were inoculated into Balb/c mice to evaluate their immune responses.
      
The protein of rSj14-3-3 was prepared and purified and BALB/c mice were immunized which was followed by a challenging infection.
      
Four hybrid clones (MM-(AB1)-1, MM-(AB1)-2, MM-(AB1)-3, and MM-(AB1)-4) were obtained by hybridoma technology involving the immunization of BALB/c mice with a BSA conjugate of aflatoxin B1 carboxymethyloxime derivative.
      
Immunization of BALB/c mice by horse antiserum against diphtheria made it possible to obtain IgG1 monoclonal antibodies (MoAbs) 2B7E4 specific for light chains of horse immunoglobulin (Ig).
      
Male BALB/c mice were irradiated with 10, 25 and 50 cGy at dose rates of 1, 5, and 15 cGy/day and mated with unirradiated females on day 15 after irradiation.
      
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This paper reports the methods and results of establishment of hybridoma cell lines secreting monoclonal antibodies (McAb) against human recombinant interferon-gamma (Hu rIFN-gamma). To gain immunized mouse spleen cells for hybridization, two groups of BALB/c mice (three mice each group) were immunized in two different projects. Mouse No. 4 with the highest serum antibody titer was used for fusion 4 days after boosting im-munization. The spleen cell suspension was made and fused with mouse myeloma cell line...

This paper reports the methods and results of establishment of hybridoma cell lines secreting monoclonal antibodies (McAb) against human recombinant interferon-gamma (Hu rIFN-gamma). To gain immunized mouse spleen cells for hybridization, two groups of BALB/c mice (three mice each group) were immunized in two different projects. Mouse No. 4 with the highest serum antibody titer was used for fusion 4 days after boosting im-munization. The spleen cell suspension was made and fused with mouse myeloma cell line SP 2/0·2Ag 14 based on the routine procedure. Culture supernatants in the wells containing hybridoma cells were screened by direct neutralization test. Two hybridoma cell lines, named A3 and B3, secreting McAb toHu rIFN-gamma were selected out. The results of crossing neutral ization test showed that the supernatants from A3 and B3 could only neutralize Hu rIFN-gamma and natural interferon-gamma,but not IFN-alpha and IFN-beta. The antibody liters of the two hybridomas were more than 128,000 and 25,600, respect-ively. The double diffusion test showed that both A3 and B3 supernatants contained mouse IgG 1. After storage in liquid nitrogen for six months, the hydridomas A3 and B3 still retained their original characteristics.

本文报道重组人γ-干扰素单克隆抗体杂交瘤细胞株建立的方法及结果。为获得供融合用的小鼠脾细胞,用两种不同的方案免疫了两组(每组3只)BALB/c小鼠。血清抗体效价最高的第4号小鼠,于加强免疫4天后取脾,制成脾细胞悬液。按照常规方法与小鼠骨髓瘤SP 2/0·Ag 14细胞融合。采用直接中和试验法筛选融合细胞孔的上清液,从中选出两株(A3和B3)分泌抗重组人γ-干扰素的杂交瘤细胞。交叉中和试验显示,A_3和B_3抗体只中和重组或自然的人γ-干扰素;与α-干扰素或β-干扰素无反应。此两株单克隆抗体的效价分别超过128,000和25,600。双扩散试验证明,A_3和B_3均为小鼠IgG_1型抗体;液氮中保存半年以上,仍保持原有的各种特性。

NIH/3T3 cells were infected with a cell-free extract from the mouse SRS asoitio tumor and propagated in vitro. Experimental results showed that type A and type C virus particles were observed in the cytoplasm of infected NIH/3T3 cells byelectron-microscopy, X-C assay and reverse transcriptase were both positive. Free provirus-DNA of the leukemia virus was found in the infected NIH/3T3 cell by Southern blot hybridization.Morphologically, the NIH/3T3 cells infected in vitro appeared spherical and formed monolayer...

NIH/3T3 cells were infected with a cell-free extract from the mouse SRS asoitio tumor and propagated in vitro. Experimental results showed that type A and type C virus particles were observed in the cytoplasm of infected NIH/3T3 cells byelectron-microscopy, X-C assay and reverse transcriptase were both positive. Free provirus-DNA of the leukemia virus was found in the infected NIH/3T3 cell by Southern blot hybridization.Morphologically, the NIH/3T3 cells infected in vitro appeared spherical and formed monolayer cell colonies of various sizes after 24-48 hours. When the cultured cells were inoculated into nude mice (BALB/c, nu nu) suboutaneously, fibrosarcoma was induced, 100%. Inoculation of the cell-free extract of the same cultured cells into inbred SW-1 newborn mice, resulted in the production of lymphocytie leukemia and lymphoma in 52.38% within 191 days.

用小鼠SRS腹水瘤的无细胞提取液感染体外培养的小鼠NIH/3T3细胞,建立可传递的、感染SRS白血病病毒(SRSV)的细胞株。感染的NIH/3T3细胞,电镜下可见A型和C型病毒颗粒,X-C检测和逆转录酶活性测定均为阳性。感染SRSV的细胞形态变圆,并出现集落性生长,给3只BALB/c(nu nu)裸鼠皮下接种,在15d内都产生纤维肉瘤。Southern blot分子杂交法证明,感染的NIH/3T3细胞内有游离的前病毒DNA存在,将其无细胞提取液注入SW-1近交系新生乳鼠,在191d内有52.38%诱发了淋巴细胞白血病和胸腺淋巴瘤。

A covenient procedure is described for monitoring antibody titers in the mouse blood. In this procedure the blood is collected from the mouse tail tip in the form of 1?00 diluted plasma and the antibody is then titrated by means of passive he-magglutination. With this procedure the anti-human C4/C4bp titers in the blood of ten BALB/c mice were followed for 14 weeks. The results demonstrated that there were obvious individual variations in the antibody production response in BALB/c mice.The results of cell fusion...

A covenient procedure is described for monitoring antibody titers in the mouse blood. In this procedure the blood is collected from the mouse tail tip in the form of 1?00 diluted plasma and the antibody is then titrated by means of passive he-magglutination. With this procedure the anti-human C4/C4bp titers in the blood of ten BALB/c mice were followed for 14 weeks. The results demonstrated that there were obvious individual variations in the antibody production response in BALB/c mice.The results of cell fusion experiments showed that the spleen cells from the actively responding mice produced more antibody-secreting hybridomas than the weak responders. .

本文介绍一种方便的监测小鼠血中抗体含量的方法。本法的要点是从小鼠尾尖采集1:100稀释的血浆,再用间接血凝反应滴定其中的抗体。用这种方法连续在14周中观察10只BALB/c小鼠血中抗人C4/C4bp抗体的变化,结果显示BALB/c小鼠产生抗体的应答反应有明显的个体差异。细胞融合实验的结果表明抗体应答反应活跃的小鼠的脾细胞产生较多的分泌抗体的杂交瘤。

 
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