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阿尔新
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  alcian
     ④Chondrogenesis was assessed with Alcian blue staining,Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry at 4,7,and 14 days after initial chondrogenic induction.
     ④在诱导后4,7,14d应用阿尔新兰染色、蕃红O/固绿染色和Ⅱ型胶原免疫细胞化学评价软骨形成情况。
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     Methods High density cultures of rat limb bud cell wereexposed to culture media containing PL(0-15y. g/ml) and culturedfor four days, Chondrogenesis was assayed based on cartilagenodule number , Alcian blue bound (OD 600) and expression of typeII collagen.
     方法:取大鼠肢芽细胞在含不同剂量PL(0-15μg/ml)的培养液中培养四天,根据培养细胞所形成的软骨集落数量、与阿尔新蓝结合量和Ⅱ型胶原的表达情况来观察分析PL促进软骨形成现象。
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     Primary RGCs expressed collagen type Ⅱ and were stained strongly for Alcian blue, and very few RGCs were positive for α-SMA.
     原代RGCs的阿尔新兰和Ⅱ型胶原染色均呈强阳性,仅个别细胞α-SMA弱阳性染色.
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     ④Staining results of ADSCs: The extracellular matrix Alcian blue staining,Safranin O/Fast Green staining and collagen Ⅱ immunohistochemistry were positive.
     ④脂肪间充质干细胞诱导后的细胞化学染色结果:诱导后脂肪间充质干细胞胞外基质阿尔新兰染色、蕃红O/固绿染色、和Ⅱ型胶原免疫细胞化学着色阳性。
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     The mucus secretion was measured bycombination of Alcian blue with glucoprotein in gastric juice.
     胃粘液测定采用阿尔新蓝(Alcian blue)与胃液中糖蛋白结合的方法。
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     Determination of nucleic acids based on the FeTSPc-Alcian blue 8GX-p-HPA-H_2O_2 catalytic fluorogenic reaction system
     四磺化铁酞菁-阿尔新蓝8GX-对-羟基苯乙酸-H_2O_2催化荧光反应体系在核酸定量中的应用
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     Ninety cases of gastric mucosa were studied by means of immunohistochemistry with monoclonal antibody MG7 and histochemistry with high iron diamine-alcian blue(HID/AB) and for nucleolar organizer region(AgNORs).
     应用胃癌单克隆抗体MG7、高铁二胺-阿尔新蓝(HID/AB)及核仁组成区(AgNORs)等免疫组织化学(免疫组化)与组织化学(组化)染色方法,对90例胃粘膜及胃癌组织进行研究。
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     There were more than 95% cells expressed collagen type Ⅱ and alcine blue stained positively in the primary RGC, as the passage number increased, the ratio of collagen type Ⅱ expression and alcine blue positive stained RGC dropped abruptly.
     原代培养的RGC中Ⅱ型胶原阳性染色细胞比例大于95%,阿尔新蓝染色也呈阳性; 随着传代次数增加阳性细胞的比例下降。
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     The retrieved implants in each animal were used for gross measurement and histological analysis. Results Gross examinations of the specimens at 6 months after implantation revealed that there was neocartilage formatin; the maxim size was 8 mm×8 mm.
     结果  6个月后聚乳酸支架维持良好 ,所有植入块的边缘均有不同程度的新生软骨形成 ,软骨细胞增生活跃 ,最大为8mm× 8mm ,阿尔新蓝染色示细胞外基质为蓝色 ,嗜碱性。
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     constructs based on nontransfected chondrocytes served as control group. Six weeks later,the subcutaneous newly formed tissues in nude mice were taken out for morphological examination,HE staining,Alice blue staining. Precollagen Ⅰ and Ⅱ mRNA expressions and mRNA expression of IGF-Ⅰ were investigated by reverse transcription polymerase chain reaction(RT-PCR) so as to estimate the structure and function of newly formed tissues.
     移植后6周,取出裸鼠皮下新生组织进行大体形态学观察(HE染色、阿尔新蓝染色),逆转录-聚合酶链反应(RT-PCR)法检测新生组织中Ⅰ型、Ⅱ型前胶原和IGF-Ⅰ基因mRNA的表达,判断裸鼠体内新生组织的结构和功能。
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  alcian
MC of different degrees of maturity were identified cytochemically on paraffin sections stained with Alcian blue-Safranine.
      
For 60 days, the MC density and the balance of Alcian-and Safranine-positive cells did not differ reliably from the intact sample.
      
The gastric specimens were prepared for microscopic view with hematoxylin and eosin (H-E) and alcian blue (A-B) stain.
      
???We investigated histologically (HE, E.v.G., α-Actin, Alcian blue/van Gieson) aortic wall tissue obtained from 107 patients (31 female, 76 male, mean age 60.9±12.8 years) with a bicuspid aortic valve.
      
The wide spaces separating collagen bundles reacted strongly with alcian blue stain in ALS patients and stained more markedly as ALS progressed.
      
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Cytochemical localization and cytophotometric determination of nuclear basic protein with heparin-Alcian blue (HAB) method and Sakaguchi's reaction specific for staining arginine-rich histone as well as scintillation measurement of ~3H-arginine incorporation in vivo and in vitro in the spermatids of rat testes were carried out for the analysis of gossypol effects on basic protein synthesis following 3,4 and 6 weeks of gossypol administration at a daily dosage of 30 mg/kg. Results indicate: 1. Cytochemically...

Cytochemical localization and cytophotometric determination of nuclear basic protein with heparin-Alcian blue (HAB) method and Sakaguchi's reaction specific for staining arginine-rich histone as well as scintillation measurement of ~3H-arginine incorporation in vivo and in vitro in the spermatids of rat testes were carried out for the analysis of gossypol effects on basic protein synthesis following 3,4 and 6 weeks of gossypol administration at a daily dosage of 30 mg/kg. Results indicate: 1. Cytochemically positive Sakaguchi reaction but negative HAB staining of nuclear basic protein were observed in the late elongated spermatids after stage 14 of normal rat testes. On the contrary, intensive nuclear HAB staining but negative Sakaguchi reaction were detectable in the spermatogonia, spermatocytes and spermatids before stage 14. This data indicated that a turnover process in the synthesis of nuclear histone rich in arginine which replaced the previous lysine-rich histone occured around the stage 14 of the late elongated spermatids.

大鼠喂服棉酚(30毫克/公斤体重/日)3周、4周和6周后,用特异显示精氨酸的坂口反应及显示富于赖氨酸碱性蛋白的肝素-阿尔新蓝(HAB)染色法,结合并合~3H-精氮酸液闪测定,对棉酚影响精子细胞核蛋白在合成与代谢上的改变,进行分析。结果表明: 1.正常大鼠14期以后的晚期精子细胞核坂口反应阳性,HAB染色阴性。相反,精原细胞、精母细胞及14期以前的精子细胞坂口反应为阴性,而HAB染色较深,表明14期前后精子细胞核蛋白有精蛋白合成的转换过程。 2.服棉酚后,部分曲细精管中14期以后的精子细胞及游离精子坂口反应变弱,并能为HAB染色。这是甲绿-焦宁染色异常的原因。也提示棉酚对晚期精子细胞核蛋白转变可能有干扰作用。 3.睾内注射~3H-精氨酸的活体实验和分离后的精子细胞与同位素温育的体外实验,均未见对照组和实验组之间有明显差异。对精子细胞变态过程中核蛋白合成上的转换的细胞化学显示方法,棉酚对这一转换过程的干扰作用的可能性及其意义予以讨论。

The effect of oleic acid on gastric mucus secretion in rats with indomethacin-induced gastric mucosal lesions was studied.The mucus secretion was measured bycombination of Alcian blue with glucoprotein in gastric juice.The results were asfollows:(1)Oleic acid 1.0 ml introduced into the jejunum of Shay rats,increasedgastric barrier mucus(P<0.01)and gastric free mucus(P<0.05)significantly.(2)A significant increase of gastric barrier mucus and cytoprotection action wasobserved when 0.25,0.5 and 1.0 ml of oleic...

The effect of oleic acid on gastric mucus secretion in rats with indomethacin-induced gastric mucosal lesions was studied.The mucus secretion was measured bycombination of Alcian blue with glucoprotein in gastric juice.The results were asfollows:(1)Oleic acid 1.0 ml introduced into the jejunum of Shay rats,increasedgastric barrier mucus(P<0.01)and gastric free mucus(P<0.05)significantly.(2)A significant increase of gastric barrier mucus and cytoprotection action wasobserved when 0.25,0.5 and 1.0 ml of oleic acid were introduced into the jejunum.Both these effects were dose-dependent.(3)Oleic acid introduced directly into thestomach was more effective in the increase of gastric barrier mucus than thatinstilled into the small intestine.(4)30% of glycerin,0.1% acetic acid or 1/15N HCl were without effect on the gastric barrier mucus secretion.These results clearly show that oleic acid stimulates secretion of gastric mucus.This effect may form a part of the mechanism involved in its cytoprotective action.

本工作研究油酸对消炎痛引起胃粘膜损伤大鼠胃粘液分泌的影响。胃粘液测定采用阿尔新蓝(Alcian blue)与胃液中糖蛋白结合的方法。将1.0ml 油酸注入到结扎幽门的大鼠空肠内,就可引起胃壁粘液及游离粘液分泌量的明显增加。以0.25、0.5和1.0ml 油酸注入到不结扎幽门的大鼠空肠内,也能显著增加胃壁粘液分泌,保护胃粘膜。这两种作用表现着剂量依赖关系。不论以油酸灌胃或注入空肠、回肠,都能明显增加胃壁粘液量,而灌胃的作用比注入肠内更明显。以1.0ml 30%甘油、0.1%乙酸及1/15N HCl 分别注入空肠,都不能刺激胃壁粘液的分泌。上述结果表明,油酸具有刺激胃粘液分泌的作用。因此,加强胃粘液分泌可能对粘膜起到屏障作用,这是油酸保护胃粘膜损伤的机制之一。

The modified Human Basophil Degranulation Test (HCDT) is a simple way to assess the degranulation of human basophils to the specific allergens. The HBDTs were performed with Artemisia pollen allergen in 50 patients with autumnal hay fever of the acute episode and remission, and in 30 healthy human adult volunteers. The positive rates were 72% in the remission of the pollinosis but only 34 % in the acute episode of the disease. No positive reactions were found in the healthy control group. The results indicate...

The modified Human Basophil Degranulation Test (HCDT) is a simple way to assess the degranulation of human basophils to the specific allergens. The HBDTs were performed with Artemisia pollen allergen in 50 patients with autumnal hay fever of the acute episode and remission, and in 30 healthy human adult volunteers. The positive rates were 72% in the remission of the pollinosis but only 34 % in the acute episode of the disease. No positive reactions were found in the healthy control group. The results indicate that the HBDT, as a sensitive and stable method, can be used in studying the mechnism of allergens. It showed that the basophils observed in these patients were decreasing rather than increasing the sensitivity to the allergen in acute attack of hay fever, This might be explained with the concept of basophil releasability. Since pollinosis is a disease characterized by the degranulation and mediator release of mast cells and basophils triggered by allergens. However, they would be relatively desensitized and respond poorly when basophils studied in vitro after hay fever attacked.

改良的人嗜碱细胞脱颗粒试验是一种观察人嗜碱细胞对特异性变应原脱颗粒反应的简易方法。其步骤包括聚蔗糖——泛影葡胺浓缩嗜碱细胞,用阿尔新蓝特异性染色细胞浆内肝素颗粒,借助于血球计数板计数未脱粒细胞,以加入特异性变应原后染色细胞减少30%以上判定为阳性。本文以蒿属花粉作为特异性变应原,对50例由蒿属花粉过敏所致的典型秋季花粉症患者作嗜碱细胞脱颗粒试验。结果发现,在花粉症缓解期阳性率为72%,且其阳性率随蒿属花粉皮试反应强度的增加而升高,而在花粉症急性发作期阳性率为34%,明显低于缓解期。由此对“嗜碱细胞释放能力”这一重要参数进行了初步探讨,认为在花粉症急性发作期嗜碱细胞在体内已经或正在和特异性变应原发生作用,此时如果嗜碱细胞在体外再和变应原接触时,由于其释放能力的限制,将处于相对的低反应状态。30例正常人的阴性反应结果则排除了蒿属花粉变应原的非特异性脱颗粒作用。本研究表明该方法在花粉变态反应研究中应用的敏感性和稳定性。

 
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