Under a suitable condition of crystallization, dark brown rhombohedron crystals (the lengths of the longest two diagonals were 0.25 and 0.12 mm, respectively) could be obtained from nitrogenase CrFe protein purified from a mutant UW 3 of Azotobacter vinelandii Lipmann grown in Cr-containing but NH 3-free medium.
① Four weeks l ater,the expression of bcl xL protein in infarcted area of cardiac myocytes de tected by immunohistochemistry showed that only a few cardiac myocytes in contro l group were stained,and most cardiac myocytes were dark brown in study group.
② The apoptotic cells were TUNEL positive in light microscopy, and the apoptotic nuclei were stained yellow brown and dark brown, which varied in size and shape from cell to cell;
(3) Mast cell is showing clearly by the combine-staining methods, and the cytoplasmic granule is deep brown, the cell nuclear is green and red blood cell is yellow;
The study,using SABC immune histochemistry technique,was designed for observing the modality,structure and distributed law of neuronal nitric oxide synthase(nNOS) positive neurons in rabbit brain. The results showed:①The nNOS positive neurons appeared deep brown which had most in the cytoplasm and the cell nucleus were more lighter;
A high concentration organic phenol-containing wastewater which is deep brown is produced in the production process of Kuihelin, in which CODCr is 2 672 mg/L and 1,4-dihydroxybenzene is 2 330 mg/L and colority is 10 000. Using ferric chloride as coagulation agent, optimum coagulant dosage and the effect of pH on coagulation are studied in this paper.
The product of reaction of C2H5SPCl2 with Co2(CO)8 was separated through column chromatography and the first band, which is deep brown in colour, was attributed to a loose a-tomic cluster of Co7(μ7-S)(μ4-PSC2H5)(μ-SC2H5)2(μ-CO)2(CO)12 with a combination of an octahedral skeleton Co4 PS and a tetrahedral skeleton Co3S by means of IR , 1H NMR , elemental analysis and XRD.
Results: The positive ra tes of TGF - β1 and AFP expression in HCC were 78.0%(32/41) and 70.7%(29/41), respectively, and were higher obviously than that in the normal liver tissue.
Immunohistochemical staining demonstrated that expression of HSP-70 in the basal and spinous cells of oral mucosal epithelia in 3 experimental groups were more intensive than that in control group. The differences of HSP-70 expression among 3 experimental groups were non-significant.
2. FN was stained dark brownish in the cytoplasma of HSC treated by LPS (10ug/ml) for 24 hours, which was much more remarkable than that in control cells.
The nitrogenous components quality fraction in both Yunnan and Xinjiang oriental tobacco varies with darkling color:brown leaves>yellow leaves>orange yellow leaves,but it is not obvious in Xinjiang oriental tabacco.
As a result, a color table was compiled for the ovaries from the beginning of development to gonad maturity, which included light yellow (sandy), yellow, beige, light purple, light brown, dark purple, brown (chocolate), and dark brown (brown).
janthostoma had shells 250 μm in height with 0.8 whorls, a bilobate velum with a dark brown pigmentation band shaped along its edge, pair of eye spots, tentacles and statocysts.
Dark brown crystals of predominantly prismatic habit with characteristic sizes of 550 × 100 × 150 μm3 were obtained at p = 4 GPa by cooling an aqueous solution in the temperature range t = 600-300°C.
is described as a heterothallic, thermophilic fungus with spherical, black, non-ostiolatecleistothecia; ellipsoidal, evanescent asci which contain eight one-celled ellipsoidal ascospores, darkening to deep brown to black, with one germ pore.
The McGovern silver stain was used to study the ground substances of the corneal stroma, which is stained a deep brown, in the absence of an epithelial cover.
By using unlabeled enzyme-immunoassay method to examine the gill disease cau-sed by the bacteria Myxococcus pisicola good results were obtained. The experimentsmanifest that the bacteria from different strains gave cross reaction to specific anti-serum, and the enzyme-immunoassay shows positive reaction. A brown color appeared,and the curved slender bacteria could easily be observed under the microscope. In thecross set, including the nomal rabbit serum, PBS-T, the absorbed serum, the Cg25strains or Cg27 st...
The horseradish peroxidase anti-horseradish peroxidase (PAP) and the avidin-biotin-peroxidase complex (ABC) methods have been used in this study to stain the neurons of the paraventricular nucleus (PVH) which reacted with the antiserum to neurophysin 1 (NP 1). Antisera to horseradish peroxidase (HRP) containing 43 mg/ml immunoglobulin G, were obtained from rabbits after inoculations with 0.1% HRP in Freund's adjuvants. PAP was prepared according to Sternberger's method with modification, and the ratio of HR...
Catalytic behavior of single component MoCl5 for the metathesis and metathesis polymerization of hexene-1 has been investigated. It was found that MoCl5 of low concentration showed catalytic activity only for olefin metathesis, at higher concentration, MoCl3 showed catalytic activity both for olefin metathesis and metathesis polymerization, while the latter was the major reaction. Addition of Bu4Sn-Ph2Si(OEt)2 to MoCl5 had distinct influence on the catalytic activity towards both two reactions. It was sugge...