Methods The tissues of 30 biopsies of condylome acuminatum in the stage of unpregnancy and pregnancy,28 biopsies of CIN (CIN Ⅰ 12,CIN Ⅱ 9,CIN Ⅲ 7) and 28 biopsies of cervical cancer(high,middle and low differentive squamons carcinoma 7,16,5 respectively) were collected and the HPV 6/11 DNA and HPV 16/18 DNA were detected by immunohistochemistry SP and in situ hybridization,respectively.
Results The prevalence of antibody to HPV 6 bL1 VLPs was much higher in patients with codylomata acuminata (75.0%Ab+;mean OD 0.111±0.094) than that in patients with cervical cancer (14.3%Ab+;mean OD 0.029±0.022) or in control subjects (2.8%Ab+;mean OD 0.012±0.024).
Methods The P53,Fas,and Bcl-2 protein were detected using immuohistochemical staining in 24 specimens of CA,46 specimens of cervical cancer and 28 specimens of normal cervical tissue. The HPV DNA of type 6,11,16,and 18 was determined by PCR.
Results The positive rate of P53,Fas,and Bcl-2 protein in CA were significantly higher than that in normal cervical tissue( P <0.001),but no significantly difference between CA and cervical cancer( P >0.05).
The expression of the oncogenes Ha-ras and C-myc was investigated in 50 cases of wart of the genital organs, 10 cases of penile intraepithelial neoplasm in 3rd grade(PIN Ⅲ). 12 cases of cervical intraepithe lial neoplasm in 3rd grade (CIN Ⅲ), 20 cases of penile cancer and 30 cases of cervival cancer with immunohistochemical method.
Loss of heterozygosity (LOH) analysis on chromosome 6 was performed to define the genetic changes that occur in the development of squamous cell cervical cancer (SCC).
The results of the works carried out in the Laboratory of Molecular Biology of Viruses, CRC in the framework of the Human Genome program and devoted to the study of the activity of cell and viral genes in cervical cancer are summarized.
Oncogenic human papillomaviruses (mostly HPV types 16 and 18) are the major cause of cervical intraepithelial neoplasia (CIN), which progresses into cervical cancer (CC).
Real-time PCR was used to quantitate structural and functional aberrations in the tumor suppressor gene RBSP3 (3p.21) in papillomavirus-positive cervical carcinoma (CC).