Thirty-one sera from operation-identified hydatid patients were tested with antigen loaded NG strips and 28 (90%) showed reaction bands with components of 60K, 36K, 32K, 24K, 20K, 17K and 12K of sheep cyst fluid antigen separated by SDS-PAGE in 5-20% gradient gel and transfered on to NC papers.
If taking the <21. 5kDa bands as positive crlteria,the positive rates of cyst fluid,whole cyst and urea soluble scolex ant1gens were 54 %,48% and 58% respectively and the false positive rates were 6%, 10% and 0% in turn.
The result of detection of effective antigens contained in cystic fluid of cysticercus cellulosae with SDS-PAGE, western blot and ELISA tech- niques, Eight antigen bands were recognized with antibodies in sera of cysticercosis patients, The molecular weights of the 8 bands were 255, 185, 105, 69, 64, 60, 48, and 41 kDa respectively.
Method Ommaya was placed in the cystic part of100patients with cystic or cystosolid craniopharyngioma guided by CT or MRI. Cystic liquid was absorbed and colloid chromic phosphate was injected. Solid part of cystosolid craniopharyngioma treated with Gamma knife.
The method is applied to the determination of Nickel and Cobalt in the hair of 64 women suffering from cervical carcinoma, in the tissues of 20 paitents with lung cancer, and in the cystic liquid of 10 women suffering from tubal carcinoma. The results so far obtained are satisfactory.
Method Plate coated by antigen of hydatid fluid,blood sera of patients were mixed with the 1∶8(+)(by ELISA)Rabbit blood sera containing cysticercus-antibody,the mixture was detected by ELISA method and blood sample with titre low than 1:2(#)was defined as the positive result.
There was no cross reaction among the McAbs of D 2G 8, D 2E 11 and E 11 H 8 with Ags of schistosoma, paragonimus, fasciolop sis, bovine filalia, hydatid fluid and cysticercus cellulosae. Only the H 3B 4 had weakly reaction with the Ag of adult schistosoma.
The accumulation of cystic fluid in each case was refractory to drainage treatment or percutaneous thanol injection therapy.
Evidence of phospholipids and mucopolysaccharides in the cystic fluid from a patient with generalized tissue calcification
A hybridoma cell line (OV632) producing monoclonal antibody against ovarian carcinomas was developed from the spleen cells of a mouse immunized with cystic fluid from a serous cystadenocarcinoma.
Specific parasite antigens, which constitute not more than 3% of total protein content of the cystic fluid, migrate, in isoelectric focusing, from a pH of less than 5 to more than 8.
Cystic fluid from 30 Greek women suffering from macrocystic disease was tested for mutagenicity in the Salmonella typhimurium mutagenicity assay using three bacterial strains in the presence or absence of liver homogenate.
As our case has shown, the association of pseudokidney appearance with cystic liquid mass must be considered as an intussusception with an enterogenous cyst.
Using spectroscopic techniques, M?ssbauer spectrometry in particular, we have demonstrated that the T1 hypersignal is due to ferritin, dissolved in the cystic liquid, after tumor cell lysis, in the course of time.
Serum samples were investigated by the serological techniques: IHAT, ELISA and Western blot using hydatid fluid antigen.
Significant increases of thymidine uptake were observed in spleen cells from infected animals exposed to protoscolex or hydatid fluid antigens.
By affinity chromatography using rabbit anti hydatid fluid IgG coupled covalently to CNBr-Sepharose 4B a protein fraction (Em 1) containing shared antigens of both parasites could be isolated from an extract ofE.
Higher percentage survival was obtained than previously reported: at 4°C, 100% survival was obtained for 20 days in medium 199 (GIBCO) and for 25 days in hydatid fluid from the host of origin.
gigantica was found common amongst the antigens prepared from hydatid cysts ingredients like germinal layer, fertile and sterile, hydatid fluid, fertile and sterile, while another protein of 34?kDa was shared between F.