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Adherence of Escherichia coli and Pro-teus mirabilis to human uroepithelial cells invitro was studied. Uroepithelial cells fromfresh morning urine of healthy young girlsand from mucous membrane of ureter of thedonors were used. The P. mirabilis strainsadhered only to squamous cells and not totransitional epithelial cells. P-fimbriated uro-pathogenic E. coli strains adhered to boththe cell types in a great quantity, whereastype-I fimbriated common E. coli strainsadhere? to either of the cell types verypoorly,... Adherence of Escherichia coli and Pro-teus mirabilis to human uroepithelial cells invitro was studied. Uroepithelial cells fromfresh morning urine of healthy young girlsand from mucous membrane of ureter of thedonors were used. The P. mirabilis strainsadhered only to squamous cells and not totransitional epithelial cells. P-fimbriated uro-pathogenic E. coli strains adhered to boththe cell types in a great quantity, whereastype-I fimbriated common E. coli strainsadhere? to either of the cell types verypoorly, but were heavily trapped by normalurinal slime. Difference in adhesion charac-teristics between the fimbriated E.coli andthe P. mirabilis may be related to the dif-ferent clinical manifestation of the urinarytract infection. Optimal test conditions wereobthained with post-log-phase bacterial cul-tures grown on the nutrient broth and nowashing of the bacteria before incubationwith the epithelial cells. The measure of theadherence of bacteria to the surface of theepithelial cells with the stained smear wasmore convenient and accurate than with thewet film,and the problem whether thecounts under microscope are those of thebacteria attached to the surface of theepithetial cells or of the inclusions in theepithelial cells could be solved. 作者测定和比较了具有P菌毛的尿道致病性大肠杆菌,具有Ⅰ型菌毛的普通大肠杆菌和奇异变形杆菌在体外粘附正常人尿道上皮细胞的能力,对测定细菌粘附能力的方法和影响细菌粘附的因素进行了讨论。 Endocrine-paracrine (EP) cells, also known as APUD or neuroendocrine cells, have been known to exist in the mammalian genito-urinary system. A fairly common localization is in the prostate and urethra. In this study we demonstrated for the first time the presence of small number of EP cells in the dissociated epithelial cells of the seminal vesicles of the guinea pig. The EP cells exhibited yellow FIF, while a few green or yellow-green FIF cells can be observed after L-dopa treatment. They were stained positive... Endocrine-paracrine (EP) cells, also known as APUD or neuroendocrine cells, have been known to exist in the mammalian genito-urinary system. A fairly common localization is in the prostate and urethra. In this study we demonstrated for the first time the presence of small number of EP cells in the dissociated epithelial cells of the seminal vesicles of the guinea pig. The EP cells exhibited yellow FIF, while a few green or yellow-green FIF cells can be observed after L-dopa treatment. They were stained positive by the Masson-Fontana's method but consistently negative with the Grimelius silver methods indicating that they were of argentaffin nature or other paracrine cells. 已知内分泌-旁分泌(EP)细胞,存在于哺乳类的泌尿生殖系统中,最常见的部位是前列腺和尿道。本研究首次证实,在豚鼠精囊腺的分离上皮中存在少量的EP细胞。这些细胞呈黄色甲醛诱发荧光;经L-dopa处理后,则有少量细胞呈绿色或黄绿色荧光。此外,精囊腺EP细胞能被Masson-Fontana银染法着色,但却呈Grimelius银染法阴性。表明精囊腺EP细胞具有亲银细胞或其他旁分泌细胞的特征。 Fourteen cases of hyposPadias and three cases of urethrostenosis were treated with transposition of scrotal flap based on the septal artery and vein. Twelve Patients were followed up for an average period of twenty months with. satisfactory results. Some anatomical study on the scrotal flap was also reported. 本文报告了应用阴囊纵隔血管蒂皮瓣修复尿道下裂14例,尿道狭窄3例.结果表明使用此皮瓣行尿道成形术,简便易行,避免了对膀胱的损伤,减少并发症,近远期效果良好。
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