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cam基因
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  cam gene
     The Role of New Calmodulin (B_0,BB,EBB) On CaM Gene Expression
     新型钙调蛋白拮抗剂(EBB、BB、B_0)对CaM基因表达的影响
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     By the methods of RT-PCR and immunohistochemistry, the expression of L1CAM gene and protein was detected in the large intestine of HD and corresponding normal tissues.
     本研究通过逆转录-聚合酶链反应技术(RT-PCR)及免疫组化方法对L1CAM基因及相关蛋白在正常肠壁组织及HD肠壁组织中的表达情况进行检测,从蛋白水平及mRNA水平观察L1CAM的表达与HD之间的关系,并由此推测L1CAM基因在HD发病原因中的作用。
短句来源
     The longer the hypertensive patients were treated by calcium antagonists,the less the mRNA of CaM gene was expressed.
     发现随着正规服用钙拮抗剂治疗时间的延长,高血压患者淋巴细胞内CaM基因mRNA的转录水平亦受到抑制。
短句来源
     EBB inhibited the expression of CaM gene on the transcription and translation level.
     同时观察到EBB引起肿瘤细胞内CaM水平降低的原因,是对CaM基因的转录产物(mRNA)和翻译产物(CaM)两方面作用的结果。
短句来源
     In this paper calmodulin (CaM) mRNA was detected in sperm for 15 fertile and 20 sterile males with digoxigenin (Dig)labeled probe of CaM gene Ⅲ cDNA. Germ cells were found in 9 sperm specimens (>4/HP) with blue positive dot, and a lot of white cells finding in one infertile male.
     本文报告了以地高辛标记CaM基因ⅢcDNA探针检测15例生育男性和20例不育男性精液中的CaMmRNA.9例出现蓝色阳性斑点的精液中均含有生精细胞(>4个/HP),其中1例不育男性精液中见有大量白细胞(>10个/HP)。
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  “cam基因”译为未确定词的双语例句
     Analysis of focal cerebral blood flow,ATP content and protein sythesis of L1 gene knockout mice
     L1CAM基因敲除小鼠的脑局部血流量、ATP含量和蛋白合成率分析
短句来源
     Objective To analyze focal cerebral blood flow,ATP content and protein synthesis of L1 knockout(L1 KO) mice and to evaluate if lack of L1 gene will have some influence on mice brain metabolism.
     目的对L1细胞黏附分子(L1 cell adhesion molecule,L1CAM)基因敲除(gene knockout,KO)小鼠的各脑区脑局部血流量、ATP含量和蛋白合成率进行分析,探讨L1CAM基因缺失对小鼠脑局部代谢功能有无影响。
短句来源
     ConclusionWithout obvious influence in brain metabolic functions,L1 KO mice is an useful animal model for investigation of the role of L1 in brain ischemia.
     结论L1CAM基因缺失对小鼠脑局部代谢功能无明显影响,提示L1 KO小鼠可作为研究L1CAM在脑缺血中作用的动物模型。
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     To know the molecular mechanism, CaM genes in peanut were cloned from both genomic DNA and cDNA using two primers designed according to the CaM conservative sequences.
     为了从分子水平上揭示Ca~(2+)-CaM系统引起的胚败育机理,本文根据CaM保守序列设计合成上、下游引物,以提取的花生幼嫩叶片总RNA(逆转录为cDNA第一链)和基因组DNA为模板,PCR扩增并克隆得到花生CaM基因
短句来源
     Cloning of Calmodulin Gene and Rearches on in Vitro Embryo Development Regulated by Calcium in Peanut (Arachis Hypogaea L.)
     花生(Arachis hypogaea L.)CaM基因的克隆与钙调控离体幼胚胚性发育的研究
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  相似匹配句对
     4 new genes were obtained.
     U基因
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     The NiCoT gene of S. aureus had been successfully expressed in E.
     外源基因在E.
短句来源
     Intelligent CAM
     智能化CAM
短句来源
     PHYSICAL LOCALIZATION OF GENES FOR CaM AND Ca2+ATPase IN RICE BY IN SITU HYBRIDIZATION
     水稻CaM和Ca~(2+)-ATPase基因的原位杂交物理定位
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     THE CAM ACTIVITY IN OROTACHYS FIMBRIATUS
     瓦松的CAM活性
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  cam 1
Comparing the compressibility data with other CaM1Si2O6 pyroxenes we suggest that the empirical K × V?=?constant relationships are followed in C2/c pyroxenes only if the same valence electron character is shared.
      
As apparent from the effect of osmotic cell shrinkage, inhibition of the NHE by CD95-receptor stimulation was absent in Lck56-deficient J-CaM1.6 cells and restored by retransfection of J-CaM1.6-cells with Lck56.
      
We have previously isolated and characterized a calmodulin gene from the early-diverging chordate Branchiostoma lanceolatum (CaM1).
      
Moreover, the CaM2 protein differs at two positions from the amino acid sequence of CaM1; the latter is identical to calmodulins in Drosophila melanogaster, the mollusc Aplysia californica, and the tunicate Halocynthia roretzi.
      
lanceolatum CaM-like genes are more closely related to the CaM2 than to the CaM1 gene.
      
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  cam gene
A new mutation of the L1CAM gene in an X-linked hydrocephalus family
      
We sequenced the coding region of the L1CAM gene of patients from two different families with X-linked hydrocephalus and found a novel mutation at nucleotide residue 1963 in one family.
      
The expression of the Drosophila calmodulin (CAM) gene is surprisingly complex.
      
The guanylate cyclase-activating proteins (GCAPs) are Ca2+-binding proteins of the calmodulin (CaM) gene superfamily that function in the regulation of photoreceptor guanylate cyclases (GCs).
      
Recent studies have presented evidence for the involvement of L1CAM gene mutations in various X-linked mental retardation syndromes.
      
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We have demonstrated tbat the distribution of microtubules(MT), microfilaments (MF) and fibroneetin(FN) were diminished, while the gene expression of the calmodulin and c-fos enhanced in the transformed C_3 H_(10) T_(10)T_(1/2) cells. After treatment with 1 mM db-cAMP for 1 hr. and 2hrs., there was an early and rapidly reduced in gene expression of calmodulin and c-fos respectively. After db-cAMP treatment for 4-5 days, the number of Capping cells of ConA binding decreased significantly and the cell surface...

We have demonstrated tbat the distribution of microtubules(MT), microfilaments (MF) and fibroneetin(FN) were diminished, while the gene expression of the calmodulin and c-fos enhanced in the transformed C_3 H_(10) T_(10)T_(1/2) cells. After treatment with 1 mM db-cAMP for 1 hr. and 2hrs., there was an early and rapidly reduced in gene expression of calmodulin and c-fos respectively. After db-cAMP treatment for 4-5 days, the number of Capping cells of ConA binding decreased significantly and the cell surface microvilli decreased also. The growth of treated cells was inhibited markedly. By using 4 F 1 cDNAprobe, which is preferentially expressed inG1 phase, we have found that the dbcAMP treated ceils were accumulated at G 1 phase. Of particular interest is the fact that the distribution of mierotubuJes, mic rofilaments and fibronectin were recovered after treatment with 1 mM db-cAMP for 6 days. It is suggested that the inhibition of proliferation, alteration of phenotype and recovery of cytoskeleton in transformed cells after treatment with db-cAMP are related to the inhibition of gene expression of calmodulin.

转化的C_3H_(10)T_(1/2)细胞表现增殖速度加快、表面微绒毛增加,细胞变圆,叠层生长,ConA受体呈帽状分布,微管、微丝、纤粘蛋白分布明显减少。与增殖有关的癌基因c-fos表达增强,同时发现与细胞增殖、转化和细胞骨架调节有关的钙调素(CaM)基因表达加强。用1mmo/Ldb-cAMP处理转化细胞,观察到CaM基因和原癌基因c-fos的表达分别在处理后1小时和2小时急剧下降。处理后4—5天,转化细胞表型趋正常化,大部分细胞恢复单层生长。细胞表面微绒毛和泡状物减少,ConA受体帽状分布消失,恢复分散分布在细胞膜上的特点。细胞生长明显被抑制,用优先在G_1期表达的4F_1 cDNA为探针进行分子杂交,证实了经db-cAMP处理后的细胞被阻抑在G_1期。经db-cAMP处理6天的转化细胞中微管、微丝、纤粘蛋白基本恢复正常分布。实验表明CaM的表达增强与转化细胞表型变化和细胞骨架组装减弱密切相关,db-cAMP作用后CaM表达下降是抑制转化细胞增殖并使细胞表型和细胞骨架分布趋于正常的关键事件之一。

Using in situ hybridization, the alteration of calmodulin(CaM geneexpression was studied in cerebral cortices of rats during hypoxia. The cell body area(area)and integral optical density(I· OD) of the hybridization signals with CaM-cDNAprobes represented the level of gene expression. As compared with normoxia group, thearea and I·OD in acute hypoxia group were 59.1%higher( P <0.01)and 45.9%higher(P<0.01),respectively; the area and I·OD in hypoxia adaptation group were 68.6%higher(P<0.01)and 60.0%higher(P<0.01),respectively....

Using in situ hybridization, the alteration of calmodulin(CaM geneexpression was studied in cerebral cortices of rats during hypoxia. The cell body area(area)and integral optical density(I· OD) of the hybridization signals with CaM-cDNAprobes represented the level of gene expression. As compared with normoxia group, thearea and I·OD in acute hypoxia group were 59.1%higher( P <0.01)and 45.9%higher(P<0.01),respectively; the area and I·OD in hypoxia adaptation group were 68.6%higher(P<0.01)and 60.0%higher(P<0.01),respectively. As compared with acutehypoxia group, the area and I·OD in hypoxia adaptation group were 5.6%higher(P<0.05) and 9.7%higher(P<0.01),respectively. These results indicated that CaM geneexpression was enhanced during hypoxia.

用原位杂交技术检测了钙调素基因(CaM-cDNA)在急性低氧、低氧适应和常氧对照大鼠脑皮质神经细胞内表达的变化,以图像分析所得胞体金颗粒面积(area)和积分光密度(I·OD)表示基因表达水平。与常氧对照组比,急性低氧组的area和I·OD分别升高50.1%(P<0.01)和45.9%(P<0.01),低氧适应组的area和I·OD分别升高68.6%(P<0.01)和60.0%(p<0.01);与急性低氧组比,低氧适应组的area和I·OD分别升高5.6%(p<0.05)和9.7%(P<0.01)。结果提示,低氧可引起CaM基因表达增强,提高mRNA转录水平,而低氧适应的作用更明显。

In this paper calmodulin (CaM) mRNA was detected in sperm for 15 fertile and 20 sterile males with digoxigenin (Dig)labeled probe of CaM gene Ⅲ cDNA. Germ cells were found in 9 sperm specimens (>4/HP) with blue positive dot, and a lot of white cells finding in one infertile male. There were none or few germ or white cells in all the specimens with negative blotting dot. Germ cells in different stages should be studied in order to discuss the relationship between CaM and male sterility on molecular level,as spermatozoa...

In this paper calmodulin (CaM) mRNA was detected in sperm for 15 fertile and 20 sterile males with digoxigenin (Dig)labeled probe of CaM gene Ⅲ cDNA. Germ cells were found in 9 sperm specimens (>4/HP) with blue positive dot, and a lot of white cells finding in one infertile male. There were none or few germ or white cells in all the specimens with negative blotting dot. Germ cells in different stages should be studied in order to discuss the relationship between CaM and male sterility on molecular level,as spermatozoa is the high specialized cell with very few cytoplasm.

本文报告了以地高辛标记CaM基因ⅢcDNA探针检测15例生育男性和20例不育男性精液中的CaMmRNA.9例出现蓝色阳性斑点的精液中均含有生精细胞(>4个/HP),其中1例不育男性精液中见有大量白细胞(>10个/HP)。所有未出现杂交斑点的精液中未见或偶见生精细胞与白细胞。作者认为,精子为含有极少胞质的高度特异化的细胞,要从分子水平上探讨CaM与男性不育的关系,需从研究各级生精细胞着手。

 
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