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    This review mainly summarizes the progress in the study on biological characteristics and laboratory detection of West Nile virus.
    本文仅简要综述西尼罗病毒生物学特性及实验室诊断的研究进展,以期为我国开展西尼罗病毒的研究与检测提供参考。
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    Objective:To construct bicistronic DNA vaccines containing extra-cellular fragment of Flt3 ligand(FL) genes and early secreted antigen(ESAT-6) of Mycobacterium tuberculosis and to express them in glomerular mesangial cells(GMC).
    目的:构建可同时表达flt3配体(flt3-ligand,FL)和结核杆菌6kD早期分泌蛋白(ESAT-6)的重组pIRES质粒,并在大鼠肾小球系膜细胞(GMC)中表达,为进一步研究结核杆菌DNA疫苗提供实验基础。
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    Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.
    结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体,为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
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    Purpose To clon the human soluble catachol-O-Methyltransferase(COMT) into the Pet22b+ vector,expression and purification the COMT protein for researching the function of COMT.
    目的将儿茶酚胺氧位甲基转移酶基因(COMT)克隆到原核表达载体进行可溶性表达,制备纯化COMT蛋白,为深入研究COMT的功能提供材料。 方法运用分子生物学方法构建融合表达质粒Pet22b+-COMT。
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    AIM: To determine the optimized condition under which pro-apoptotic gene expression vectors with CMV and hTERT promoter will be constructed to transfect salivary adenoid cystic carcinoma cells (SACC-83), plasmid vectors coding enhanced green fluorescence protein (EGFP) gene pACCMV-EGFP and pACTERT-EGFP were transfected into SACC-83. The transfection efficiency and transient expression were subsequently tested.
    目的:检测绿荧光蛋白基因(EGFP)质粒表达载体pACCMV-EGFP和pACTERT-EGFP对SACC-83的转染效率及瞬时表达,为应用这两种启动子诱导促凋亡基因转染SACC-83以发挥治疗作用提供依据。
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1. Therty-three adult male rabbits were used for this experiment. The animals were divided into four groups. In the first experimental group, a piece of its own spleenic tissue was implanted to the myocardial surface in each animal after the internal mammary arteries had been ligated bilaterally. Within one to four weeks after the first operation, the animals were subjected to ligation of the circumflex branch of the left coronary artery. Out of 18 animals, 12 survived over 5 days after 2nd operation. In the...

1. Therty-three adult male rabbits were used for this experiment. The animals were divided into four groups. In the first experimental group, a piece of its own spleenic tissue was implanted to the myocardial surface in each animal after the internal mammary arteries had been ligated bilaterally. Within one to four weeks after the first operation, the animals were subjected to ligation of the circumflex branch of the left coronary artery. Out of 18 animals, 12 survived over 5 days after 2nd operation. In the second experimental group the same procedure was repeated as group 1, except the implantation of the spleenic tissue. Two animals survived out of 5 operated. In the control groups (the 3rd and 4th groups) no operation was performed before ligation of the branches of the coronary artery. In the 3rd group, after the ligation of the ant. descending branch, 3 out 4 animals survived over 5 days, while in the 4th group, after ligation of circumflex branch none survived over 5 days in 6 animals operated. 2. In group 2, X-ray photographs indicate that collateral circulation was established through the anastomosis between pericardial vessels and branches of the coronary artery. 3. The X-ray photographs of the experimental group 1 proved the establishment of collateral circulation between ant. descending branch and circumflex branch of the left coronary artery by the vasculature in the spleenic implantation. 4. As is shown by periodic acid Schiff reaction, (1) in both control groups, the glycogen granules in the area of coronary occlusion were more reduced than in the border area, (2) in the second experimental group the glycogen granules in the area of coronary occlusion have slightly increased in comparison with those in same area in the control group and (3) in the first experimental group the glycogen granules in the area of coronary occlusion were increased more than in the same area in the second group. These changes offer convincing elucidation that in the first and second groups, owing to the production of the intercoronary collateral circulation or the hemodynamic effect, the blood supply to the area of the coronary occlusion was ificreased.

1.第一组实验结果证明,于左冠状动脉的二枝之间,在实验性乳房内动脉结扎后所产生的血液循环动力学效应的基础上借移植脾内的血管建立了侧枝循环。为冠状动脉急性闭锁区提供了足够的血液供给。使残恬五天以上的动物提高到66.6 % o 2.第二组的实验结果说明,在结扎乳房内动脉后所产生血液动力学(hemodynamic)的效应通过心包隔动脉侧压(lateral-pressure)和血流量的增加而开伐和凿通了心包血管床并经心包翻拆处与冠状动脉系统相交通。对冠状动脉闭锁区的心肌表层提供了一定的血液量。3.在各组动物冠状动脉的闭锁区用PAS反应方法所显示的糖原颗粒的变化进一步地证实了第二组闭锁区的贫血状况得到一定的改善而第一组获得了此较彻底地改善。

Subjects of this study consisted of 700 Chinese healthy youths of 19--25 years of

就19—25岁的健康青年700人(男女各半)的手,从掌侧面进行了测量。以指长等级为标准将1400只手分为七型。其中Ⅰ(短)、Ⅱ(中)、Ⅲ(长)型为常见型。在女性,Ⅰ型为主型,Ⅱ型力次型;在男性,Ⅱ型为主型,Ⅲ型为次型。手掌各径的长度,多数随手型的长短而增减。男性手的各径,绝大多数均大于女性,并有非常显著的差别。女性手较男性手瘦长而薄。手的指数在女性平均为50.85,男性为52.57。掌的指数在女性为89.35,男性为92.00。左右手之间,无论男女,除掌厚度为右厚于左外,其余均无明显差异。根据测量结果,归纳出三型常见手的数据,为我国的机械装备、工具握柄和衣著的设计,提供一些参考资料。

A procedure for phenotyping hyperlipoproteinemia is presented. 0.1 ml of a saturated solution of Sudan Black-B in petroleum ether-alcohol solvent (1:4) and 0.05 ml absolute ethyl alcohol was added to 1 ml of serum. After incubation for 20 min at 37℃ and removal of excess dye by centrifuging, 0.04 ml of the stained serum was applied to filter paper for electrophoretic separation. The optimal conditions for paper electrophoresis were an albuminated "tris" buffer, pH 8.9, ionic strength 0.42 M in terms of tris...

A procedure for phenotyping hyperlipoproteinemia is presented. 0.1 ml of a saturated solution of Sudan Black-B in petroleum ether-alcohol solvent (1:4) and 0.05 ml absolute ethyl alcohol was added to 1 ml of serum. After incubation for 20 min at 37℃ and removal of excess dye by centrifuging, 0.04 ml of the stained serum was applied to filter paper for electrophoretic separation. The optimal conditions for paper electrophoresis were an albuminated "tris" buffer, pH 8.9, ionic strength 0.42 M in terms of tris with EDTA and boric acid concentrations at 0.017 M and 0.061 M respectively; potential gradient 5~6 volts/cm and a current of 0.75~1 mA/strip.

近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75~1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2...

近年来冠心病的发病率和死亡率很高,青中年人中发病率亦日趋增多,因此冠心病成为现代医学研究的重大课题。作者初步建立了一个重复性较好,操作简便,费用经济,适用于临床实验室的脂蛋白纸上电泳预染分型法.以便为高脂蛋白血症分型提供较多的线索。一、用苏丹黑B石油醚-无水乙醇饱和溶液先染血清,后在含1%白蛋白的TEB(三羟甲基氯基甲垸-乙二胺四乙酸-硼酸)缓冲液中,电压160V,电流0.75~1.O毫安/滤纸条,电泳16小时,能使40微升正常血清在滤纸上出现除原点外还有三条着色区带,即β-脂蛋白,前β-脂蛋白及α-脂蛋白。正常人脂餐后,在原点与β-脂蛋白间还有乳糜微粒及前β-脂蛋白“拖尾”区带出现。二、用门诊数十名患者血清,分别电泳20次观察方法的重复性。结果:β-,前β-及α-脂蛋白相应地为43.O±O.42(S.E.)%,33.7±0.52(S.E.)%及23.4±0.47(S.E.)%。三、测定了33名(男18名,女15名),平均年龄为43.7岁,一般健康良好,无脂类代谢疾病的本医学院教职工的脂蛋白:α-,β-和前β-脂蛋白相应为187±42毫克/100毫升,241.3±34.2毫克/100毫升及75.2±29.3毫克/100毫升。四、讨论了不同类型高脂蛋白血症中脂蛋白的异常及分型在临床应用上的意义。

 
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