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参照基因
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  reference gene
     The target gene and internal reference gene glyceraldehydes-3-phosphate dehydrogenase (GAPDH) were amplified together.
     统计扩增的目的基因片段与内参照基因片段比值 ,并与RA患者疾病活动指标行相关性分析。
短句来源
     Conclusion:This gene and method can be used to detect the endogenuous reference gene in wheat.
     结论:该基因与方法可用作检测小麦属(唯一小麦种是栽培作物)内源特异参照基因
短句来源
     A set of commonly used primers of patatin sequence of potato were designed as endogenuous reference gene,and check on the quality of template extraction from potato,and avoid the result of false negative.
     该方法根据马铃薯自身patatin基因作为内源特异参照基因扩增216bp片段,检查模板DNA提取的质量,避免了假阴性结果;
短句来源
     Methods: In this study, we chose the reference gene on the same chromosome as the target gene, and used the differential polymerase chain reaction (d-PCR) to assess the presence of c-erbB-2 gene amplification in frozen sections of breast cancer.
     为了能够快速、简便、准确的检测乳癌中c-erbB - 2基因的扩增 ,我们将靶基因与参照基因设在同一条染色体上 ,同时选用快速冰冻切片的剩余组织作d -PCR实验。
短句来源
     In addition, a multiplex PCR method was developed by which specific fragments with different length in a DNA sample mixture isolated from six transgenic maize could be amplified simultaneously in one PCR reaction, meanwhile, one fragment of maize endogenous reference gene zein could also be detected.
     在此基础上建立了针对6种转基因玉米的特异性DNA片段和玉米内参照基因(zein)的复合PCR检测方法,能同时对7对引物进行扩增,通过产物的长度差异对不同的转基因玉米进行辨别,实现了在同一个PCR反应管中同时对6种不同转基因玉米的特异性检测。
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  reference genes
     Methods Using the coamplification of target and reference genes. One pair of specific primers were designed according to a portion of the dextranase(dexA) gene of S.mutans . The reference gene was plasmid pET23b DNA.
     方法 采用靶基因与参照基因同步扩增法 ,根据变形链球菌葡聚糖酶 (dexA)基因序列 ,设计一对特异性引物 ,以pET2 3b质粒DNA为参照基因
短句来源
     Methods Using the coamplification of target and reference genes. One pair of specific primers were designed according to a portion of the dextranase(dexA) gene of S. Mutans. The reference gene was plasmid pET23b DNA.
     方法 采用靶基因与参照基因同步扩增法 ,根据变形链球菌葡聚糖酶 (dexA)基因序列 ,作者设计一对特异性引物 ,以pET2 3b质粒DNA为参照基因
短句来源
     Results The grain-specific endogenuous reference genes w ere amplified successfully when using DNAs extracted with modified CTAB method. 0.5% RRS and 0.5% Bt? 176 Maximaizer could be identified by PCR detecting c amv 35?
     结果 改良CTAB法制备的DNA用作PCR模板均可扩增出内参照基因 ,PCR扩增camv35S启动子可检测出含量为 0 5 %的RRS和Bt176Maximaizer,而PCR扩增nos终止子可检测出含 1%RRS的食品样品。
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  “参照基因”译为未确定词的双语例句
     WT1 and AML1-ETO levels were normalized by internal control ABL gene.
     WT1及AML1-ETO基因表达水平用内参照基因ABL归一化。
短句来源
     ⑤The electrophoresis band positions of myocardial CYP11B1 and CYP11B2 as well as RT-PCR products of glyceraldehydes-3-phosphate dehydrogenase (GAPDH), the consulting gene, were in conformity with the theoretic values.
     ⑤各组大鼠心肌组织CYP11B1和CYP11B2及参照基因甘油醛-3-磷酸脱氢酶基因的反转录聚合酶链反应产物电泳带的位置与理论值相符。
短句来源
     Methods The expression level of the three TGF β sub type mRNA,which were TGF β1,TGF β2 and TGF β3,was measured by quantitative Reverse Transcription Polymerase Chain Reaction(RT PCR)with the β actin as the internal control.
     方法 采用RT PCR技术 ,以 β actin基因为内参照基因 ,分别确定TGF β基因亚型 ,包括TGF β1、TGF β2、TGF β3在HTS组织中的表达 ,及其表达与病程的关系。
短句来源
     The ratio of copy number between Bm Tpi and DH-PBAN, which is located on the 11th chromosome was detected with the real-time quantitative PCR technique. It was 1.0 in males and 0.5 in females.
     采用实时定量PCR技术,以家蚕Bombyxmori第11号染色体上的DH-PBAN基因为参照基因,检测家蚕不同个体间BmTpi基因与常染色体上DH-PBAN基因的拷贝数之比,雄体BmTpi∶DH-PBAN=1.0,雌体BmTpi∶DH-PBAN=0.5;
短句来源
     Detection of endogenous specific BnACCg8 gene and E9 3' terminator in genetically modified roundup-ready rapeseed
     转基因抗草甘膦油菜内源特异参照基因BnACCg8和外源E93'终止子的检测研究
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  reference gene
We measured two genes simultaneously, ST13 as the target gene and glyceraldehydes-3-phosphate dehydrogenase as a reference gene, in primary colorectal tumor specimens and tumor-adjacent normal mucosa specimens from 50 colorectal cancer patients.
      
Glyceraldehyde phosphate dehydrogenase mRNA in the same samples was used to relate the AT1 mRNA content to a stably expressed reference gene.
      
Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization
      
This Bt rice specific detection system was combined with a recently published quantitative real-time PCR method for the rice-specific (Oryza sativa L.) reference gene gos9.
      
In addition, combined with a reference gene and using reference standard material, the method can be used to semiquantitatively estimate the amount of gm plants in an unknown sample.
      
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  reference genes
Valid reference genes (HKGs) were identified using GeNorm and NormFinder software packages and by direct comparison of Ct values.
      
Using quantitative reverse transcription-polymerase chain reaction (RT-PCR), reference genes are utilized as endogenous controls for relative quantification of target genes in gene profiling studies.
      
The objective of this study was to select from a panel of 16 potential candidate reference genes the most stable genes for gene normalization.
      
All other genes did not differ between the matched pairs, and the software programs geNorm and NormFinder were used to ascertain the most suitable reference genes from these candidates.
      
The expression of the target gene RECK normalized with HRPT1 alone and with the normalization factors generated by the combination of these three reference genes as well as with the unstable genes ACTB or RPL13A is given.
      
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Abstract Oncogene amplification is found in many human tumors,and may indicate

癌基因的扩增见于多种人类肿瘤,有预后价值。本文报告了应用差别(D-)PCR技术,检测人卵巢癌组织C-erb-B2基因拷贝数的改变。在这一技术中,靶基因(C-erb-B2基因)和单拷贝参照基因(IFNG),在同一反应管中进行扩增,靶基因拷贝数为靶基因和参照基因扩增产物带晁密度的比值。在本组11例卵巢癌标本中,有1例存在C-erb-B2癌基因低拷贝数的扩增,并对10份送检的卵巢肿瘤组织和同源正常组织同时作印迹转变分析,和D-PCR检测结果一样,未发现有C-erb-B2癌基因的扩增。D-PCR检测癌基因的扩增快速、简便,无需应用放射性同位素,具有较好的应用价值。

Purpose: The amplification of HER-2/neu may have prognostic value in breast cancer. Methods: In this study, we chose the reference gene on the same chromosome as the target gene, and used the differential polymerase chain reaction (d-PCR) to assess the presence of c-erbB-2 gene amplification in frozen sections of breast cancer. Results: The results showed that there were 19 of 71 cases of breast cancer were detected amplification of c-erbB-2 gene, the detectable rate was 26.7%, which was higher than our previous...

Purpose: The amplification of HER-2/neu may have prognostic value in breast cancer. Methods: In this study, we chose the reference gene on the same chromosome as the target gene, and used the differential polymerase chain reaction (d-PCR) to assess the presence of c-erbB-2 gene amplification in frozen sections of breast cancer. Results: The results showed that there were 19 of 71 cases of breast cancer were detected amplification of c-erbB-2 gene, the detectable rate was 26.7%, which was higher than our previous reports. Conclusion: the author regards that the chose of primer and the specimens contribute to the improvement of the detectable rate.

目的与方法 :c-erbB - 2基因的扩增与乳腺癌的治疗和预后有一定的关系。为了能够快速、简便、准确的检测乳癌中c-erbB - 2基因的扩增 ,我们将靶基因与参照基因设在同一条染色体上 ,同时选用快速冰冻切片的剩余组织作d -PCR实验。结果 :研究结果表明 ,71例乳腺癌中共有 19例c -erbB - 2基因的扩增 ,扩增率为 2 6 .7%。与我们过去的研究相比 ,c-erbB -2基因扩增检出率有所提高。结论 :本文认为 ,引物设计合理、选材得当 ,能够提高c-erbB - 2基因扩增的准确检出率。

Objective: To investigate the value of diagnosis microarray in detection of hepatitis B and C virus. Methods: The conservative sequences of hepatitis B and C virus were used as probe. The non homology sequences of vegetative genes were used as control. When it was used, the patient serum was abstracted. The viral nucleotides were reversely transcribed and enlarged by polymerase chain reaction (PCR). Then it was labeled with fluorescence, and hybridized with the diagnosis genechip. The genechips were scanned...

Objective: To investigate the value of diagnosis microarray in detection of hepatitis B and C virus. Methods: The conservative sequences of hepatitis B and C virus were used as probe. The non homology sequences of vegetative genes were used as control. When it was used, the patient serum was abstracted. The viral nucleotides were reversely transcribed and enlarged by polymerase chain reaction (PCR). Then it was labeled with fluorescence, and hybridized with the diagnosis genechip. The genechips were scanned using scanner (ScanArray 3000). Results: The diagnosis genechip included positive and negative controls. All kinds of serum (normal, E antigen positive, HCV RNA positive, mixed serum of HBV and HCV) could be detected. Conclusion: Diagnosis genechip for HBV and HCV can be used for clinic diagnosis after operators are trained. [

目的 :探讨研制检测乙型和丙型肝炎病毒的基因芯片。方法 :以 HBV、HCV高度保守的基因片段为探针 ,同时用没有同源性的植物基因片段为内参照基因 ,制备成“乙、丙型肝炎病毒双检基因芯片”。检测时抽提患者血清中的病毒核酸 ,进行RT- PCR扩增及荧光标记 ,然后与双检基因芯片杂交 ,杂交结果用 Scan Array 30 0 0扫描仪扫描。 结果 :双检基因芯片的内参照结果能做到绝对阳性和绝对阴性 ,同时对不同血清 (正常人血清 ,乙肝 E抗原阳性血清 ,丙肝 RNA阳性血清 ,乙肝、丙肝阳性混合血清 )能有效检测区分。 结论 :本研究成功地制作了乙、丙型肝炎病毒双检基因芯片 ,可望使用于临床

 
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