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再生基因
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  “再生基因”译为未确定词的双语例句
     Detection and localization of Nogo-A mRNA expression in cultured oligodendrocytes isolated from optic nerve using in situ hybridization
     原位杂交定位检测神经抑制再生基因Nogo-A mRNA体外培养的视神经少突胶质细胞的表达
短句来源
     Rapid induction of mRNAs for liver regeneration genes by hepatopoietin and partial hepatectomy
     肝细胞生成素及肝部分切除诱导肝再生基因PC3的表达
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     Advances in the Studies of Reg Family
     再生基因家族的研究
短句来源
     The results of this research were listed as following:1 .The high frequency regeneration system of tetraploid black locust has been established.
     1.建立了四倍体刺槐高频再生基因转化受体系统。
短句来源
     Since the first member of Reg gene was discovered in 1988,it has been verified that Reg genes play important roles in diabetes,inflammation and injury,and tumors.
     再生基因家族自1988年被发现以来,其在糖尿病、炎症创伤与肿瘤尤其在消化系统肿瘤中的作用日渐被重视。
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Advances in the Studies of Reg Family
     再生基因家族的研究
短句来源
     Periodontal Regeneration by Gene Therapy
     基因治疗在牙周组织再生中的应用
短句来源
     Overexpression of AK fbr gene in C.
     AKfbr基因在C.
短句来源
     Regenerated Polyester Fibres
     再生聚酯纤维
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Since the first member of Reg gene was discovered in 1988,it has been verified that Reg genes play important roles in diabetes,inflammation and injury,and tumors.More members were cloned and their application in treatment was studied.With the development of related research,there is a great potential of Reg family in biomedical field.

再生基因家族自1988年被发现以来,其在糖尿病、炎症创伤与肿瘤尤其在消化系统肿瘤中的作用日渐被重视。越来越多的该家族成员被发现,并已开始考虑在临床治疗中应用。这些研究开始显示再生基因家族的潜在应用价值。

Objective To investigate the amelogenin expression of recombinant Plasmids PsecTaq2A-AMG in human gingival fibroblast cell line. Methods Primary human fibroblasts were cultured and transfected with PsecTaq2A-AMG. The cells and cultured supernant were colllected for the detection of expression of amelogenin on day 0,2,5,7,14 by ELISA test. Results The AMG protein concentration was detected until 14 day, and peaking on the 5th and 7th day. Conclusions The results thus provided bases for the gene transfer of...

Objective To investigate the amelogenin expression of recombinant Plasmids PsecTaq2A-AMG in human gingival fibroblast cell line. Methods Primary human fibroblasts were cultured and transfected with PsecTaq2A-AMG. The cells and cultured supernant were colllected for the detection of expression of amelogenin on day 0,2,5,7,14 by ELISA test. Results The AMG protein concentration was detected until 14 day, and peaking on the 5th and 7th day. Conclusions The results thus provided bases for the gene transfer of amelogenin into human gingival fibroblast, which may give insight for the gene therapy for periodontal regeneration.

目的 研究釉原蛋白重组质粒PsecTaq2A -AMG在人牙龈成纤维细胞 (GFB)中的表达 ,为牙周再生的基因治疗奠定基础。方法 采用酶解 -组织块法体外培养得到人牙龈成纤维细胞 ,用釉原蛋白重组质粒PsecTaq2A-AMG瞬时转染牙龈成纤维细胞 ,分别在第 4 8小时、第 5天、第 7天、第 14天收集细胞及细胞液 ,经ELISA连续测定釉原蛋白的表达。结果 转染后 4 8小时即检测到表达产物 ,表达量在第 5天和第 7天达到高峰 ,持续到第 14天仍可检测到釉原蛋白的表达。结论 实验结果为利用牙龈成纤维细胞为靶细胞 ,将釉原蛋白基因直接导入牙龈成纤维细胞内实现牙周再生的体内基因治疗奠定了基础

Objective To construct the eukaryotic expression clone for human amelogenin. Methods Total RNA was isolated from human fetal tooth buds. RT-PCR was used to amplify the amelogenin encoding region, and the amplified fragment for human amelogenin was inserted into eukaryotic expression vector PcDNA 3.1. The positive clones were selected and analyzed by restriction endonuclease mapping and DNA sequencing. Results 570 bp fragment was produced by RT-PCR; it was of the same size as expected based on human ameloginin...

Objective To construct the eukaryotic expression clone for human amelogenin. Methods Total RNA was isolated from human fetal tooth buds. RT-PCR was used to amplify the amelogenin encoding region, and the amplified fragment for human amelogenin was inserted into eukaryotic expression vector PcDNA 3.1. The positive clones were selected and analyzed by restriction endonuclease mapping and DNA sequencing. Results 570 bp fragment was produced by RT-PCR; it was of the same size as expected based on human ameloginin mRNA encoding area length. The sequence of the inserted fragment from the recombinant clone PcDNA 3.1-AMG was consistent with that of AMELX from GenBank with one mismatch on 485 from G to C, without affecting the amino acid sequence. Conclusion The eukaryotic expression clone PcDNA3.1-AMG was successfully constructed with the properly inserted DNA sequence encoding mature human amelogenin.

目的 构建人釉原蛋白基因真核表达克隆。方法 从胚龄 2 0周的引产胎儿牙胚中提取总 RNA,RT- PCR法扩增人釉原蛋白编码区基因 ,重组到真核表达载体 Pc DNA3.1中 ,经酶切和 DNA序列分析验证。结果经 RT- PCR扩增后 ,得到大小约 5 70 bp的特异性产物 ,与预期的人釉原蛋白 m RNA编码区碱基长度一致 ,重组克隆 Pc DNA3.1- AMG的测序结果显示 ,与 Gen Bank中的人釉原蛋白基因 (AMEL X)序列仅在第 4 85位碱基发生错配 (G→ C) ,但不影响氨基酸组成。结论 本研究成功的构建了人釉原蛋白基因真核表达克隆 ,为进一步研究牙周组织再生的基因治疗奠定了基础。

 
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