Aim: To investigate apoptosis-induced effect of an antimalarial drug artesunate in hepatoma cell line HepG2. Methods : The apoptosis was detected by MTT assay, flow cytometry, ladder DNA electrophoresis and transmission electromicroscopy.
AIM: To investigate the apoptosis induction effect of the expression of recombinant Caspase-3 on laryngocarcinoma cell line Hep-2. METHODS: Recombinant Caspase-3 gene was subcloned into the GFP reporting vector pEGFP-C1 to generate the expressing vector pEGFP-Caspase-3 by DNA recombinant technique.
Objective:To investigate the growth inhibition and apoptosis induction effect of vitamin E succinate (VES) on MDA-MB-435 human breast cancer cells and to analyze the changes of Fas expression in this process.
Objective To investigate the apoptosis induction effect of recombinant caspase-3 expression on osteosarcoma cell line SOSP-9901. Methods Recombinant caspase-3 gene was subcloned into the GFP reporter vector pEGFP-C1 to generate the expression vector pEGFP-caspase-3 by DNA recombinant technique. pEGFP-caspase-3 was transfected into human osteosarcoma cell line SOSP-9901 by Lipofectamine 2000. The expression of recombinant caspase-3 was determined by RT-PCR.
Recently tanshinone IIA has been reported to have apoptosis inducing effects on a large variety of cancer cells.
Hybridoma cells overexpressing the apoptotic suppresser gene bcl-2 were protected from the predominantly necrosis inducing effects of Mg2+ ion deprivation and apoptosis inducing effects of Ca2+ ion deprivation.
We studied the apoptosis inducing effects on 3 tumor cell lines by FasL and anti-DR5 mAb, and the underlying mechanism.