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干涉效果
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  interference effect
     Interference Vector Construction of nanog Gene of ES-D3 Cells and Their Interference Effect
     ES-D3细胞nanog基因干扰载体的构建及其干涉效果
短句来源
     Then SKBr-3 cells were transfected with pSUPER-S1 or pSUPER-S2, together with pCDNA3 plasmid, and subjected to G418 selection. In G418-resistant cells, the interference effect was detected by RT-PCR, Western blot and immunocytochemical staining.
     通过脂质体介导,把重组质粒稳定转染入SKBr-3细胞,RT-PCR、Western blot印记杂交和细胞免 疫化学法检测其对mRNA和蛋白表达的干涉效果
短句来源
     In order to analyze the imaging properties of this Line-scanning OCT system,Zemax,a powerful optical design software was used to simulate the interference effect of this system.
     为验证基于线扫描方式的时域OCT系统的可行性及得到其设计基本参数,借助Zemax光学设计软件模拟该系统的干涉效果,分析线扫描时域OCT的成像特性。
短句来源
  interfering effect
     HeLa cells were then transfected with pSilencer-S1 and pSilencer-S2. After G418 selection, the cells were selected and the interfering effect was detected by RT-PCR.
     脂质体法转染重组质粒入HeLa细胞,G418筛选后RT-PCR检测其对stathmin基因mRNA的干涉效果.
短句来源
     The RNA interfering effect in fetal fibroblasts was markedly higher than that in the myogenic Satellite cells (P<0.05). Conclusion: The three recombined interfering plasmids could suppress the expression of the mRNA of myostatin gene in porcine.
     两种细胞间的RNA干涉效果差异显著(P<0.05),Myostatin基因在仔猪骨骼肌卫星细胞中的RNA干涉效果显著低于猪胎儿成纤维细胞中的RNA干涉效果
短句来源
  “干涉效果”译为未确定词的双语例句
     The purpose of this study is to find appropriate small interfering RNAs (siRNAs) targeting HER-2 for breast cancer gene therapy. First, six sequences of human HER-2 mRNA were obtained from GenBank.
     虽然对RNAi药物的研究才刚起步,但已取得很大进展。 本研究的目的就是找到具有较好干涉效果的小干涉RNA(small interfering RNA,siRNA),实现对乳腺癌细胞中HER-2表达的高效抑制,并最终用于对HER-2高表达乳腺癌的基因治疗。
短句来源
     To construct effective RNAi analysis technology in embryo of bombyx mori basing on predecessor, this article reviews the effect of RNA interference in different breeds and different methods of dsRNA mediated used the Bmwh3 gene, and researches the suitable dosage and stage of injecting dsRNA.
     为在家蚕胚胎发育时期建立有效的RNAi分析技术,本文在前人的基础上以家蚕Bmwh3为材料,探讨了不同导入方法对RNA干涉的影响及RNAi在华系大造品种中的运用,并比较了两种导入剂量和两种导入时间的RNA干涉效果的差异。
短句来源
     In chapter two the theory of Mach-Zehnder interferometer is depicted. The influence of circumstance on Mach-Zehnder interferometer is analyzed too.
     首先文章阐述了光纤Mach-Zehnder干涉仪的基本原理,并从理论上分析环境因素对于光纤Mach-Zehnder干涉仪干涉效果的影响。
短句来源
     The paper deals with the interrference field of the optical fiber Mach_Zehnder interferometer. An analysis was given in which all the factors influencing the result were studied. A good way was found to get a better interference fringes.
     研究了光纤Mach_Zehnder干涉仪的干涉场的特性 ,并对影响干涉效果的因素进行了分析 ,得到了干涉条纹的分布及获得高对比度干涉条纹的方法
短句来源
     We confirmed that siRNA whose sense-stranded siRNA elements carry two nucleotide mismatches at the 3′-ends against the antisense-stranded siRNA elements, has an equal RNAi activity to the conventional siRNA.
     结果初步证实正义链3′碱基错配的siRNA也具有显著的干涉效果
短句来源
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  相似匹配句对
     The Experiment and Effeet in Michelson Interference
     迈克尔逊干涉实验及其效果
短句来源
     What about the effect?
     效果如何?
短句来源
     The result is good.
     效果是好的.
短句来源
     RNA Interference
     RNA干涉
短句来源
     Interference Vector Construction of nanog Gene of ES-D3 Cells and Their Interference Effect
     ES-D3细胞nanog基因干扰载体的构建及其干涉效果
短句来源
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  interference effect
The interference effect in the photoelectron energy spectrum during atomic ionization by a periodic field of a general form is examined.
      
Nonlinear interference effect in the Zeeman ion laser
      
The nonlinear interference effect in the Zeeman laser is calculated taking account of Coulomb diffusion in velocity space.
      
Previously, our group described a quantum-interference effect ("valve effect") to be expected in the resonant scattering of M?ssbauer photons in the NMR mode.
      
The absorption spectrum of the probing field components contains parametric supernarrow resonances against the background of resonances of the nonlinear interference effect.
      
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  interfering effect
The interfering effect of mono- and diisobutyl methylphosphonates (matrix components formed on the destruction of ME) is eliminated by extraction and back extraction.
      
The interfering effect of lead(II) was minimized by selecting an appropriate accumulation medium.
      
It was demonstrated that the interfering effect of petroleum products on the results of analysis can be eliminated by using an additional chromatomembrane cell.
      
The interfering effect of organic compounds dissolved in natural water is eliminated by the ultrasonic treatment of water samples.
      
The conditions of the formation of sensitive films (modifier weight and solvent) were optimized, and the interfering effect of alkyl acetates was studied.
      
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A contribution relationship of conservation law M-Integral along a far field path between a main-crack and micro-cracks around the main-crack tip is proposed. The expression of this contribution relationship is derived, which gives the affection of the distances between main-crack and micro-cracks on interaction effects. The discussion gives an ideal method for assessing numerically the interaction effects. Then the M-integral assessment theory is established.

由M守恒积分给出了其远场积分值与宏观裂纹及其裂尖周围裂纹群应力强度因子间的分配关系,导出了分配表达式,该式首次显化了裂纹间的距离对裂纹间干涉效果、裂尖应力松弛的影响,是定量评估裂纹干涉效果的一个较为理想的方法。建立了裂纹干涉效果定量分析的一种新方法———M积分评价理论。

The paper deals with the interrference field of the optical fiber Mach_Zehnder interferometer. An analysis was given in which all the factors influencing the result were studied. A good way was found to get a better interference fringes.

研究了光纤Mach_Zehnder干涉仪的干涉场的特性 ,并对影响干涉效果的因素进行了分析 ,得到了干涉条纹的分布及获得高对比度干涉条纹的方法

Objective: To construct the eukaryotic expression vector for RNA interferencing human survivin gene and detect its interference effect in human breast cancer cell line(SKBr-3). Methods: Two target gene segments were synthesized and cloned into pSUPER vector respectively to construct two recombinant eukaryotic expression vectors: pSUPER-S1 and pSUPER-S2. The two recombinant vectors were identified by enzyme digestion analysis and DNA sequencing. Then SKBr-3 cells were transfected with pSUPER-S1 or pSUPER-S2,...

Objective: To construct the eukaryotic expression vector for RNA interferencing human survivin gene and detect its interference effect in human breast cancer cell line(SKBr-3). Methods: Two target gene segments were synthesized and cloned into pSUPER vector respectively to construct two recombinant eukaryotic expression vectors: pSUPER-S1 and pSUPER-S2. The two recombinant vectors were identified by enzyme digestion analysis and DNA sequencing. Then SKBr-3 cells were transfected with pSUPER-S1 or pSUPER-S2, together with pCDNA3 plasmid, and subjected to G418 selection. In G418-resistant cells, the interference effect was detected by RT-PCR, Western blot and immunocytochemical staining. Results: Enzyme digestion analysis and DNA sequencing showed that the target segments were cloned into pSU PER vector respectively. The results of RT-PCR, Western blot and immunocytochemical staining indicated that both vectors could knock down the transcription and expression of survivin gene, and that pSUPER-S1 had better interference effect than pSUPER-S2. Conclusion: The transcription and expression of survivin gene were inhibited effectively by the constructed RNAi eukaryotic expression vectors in the breast cancer cells.

目的:构建针对人survivin基因的siRNA真核表达载体,检测其对人乳腺癌SKBr-3细胞中survivin基因表达的干 涉作用。方法:将合成的寡核苷酸链退火形成双链,连接入经HindⅢ和BglⅡ双酶切后的pSUPER真核表达载体。对重组质 粒进行酶切分析和测序鉴定。通过脂质体介导,把重组质粒稳定转染入SKBr-3细胞,RT-PCR、Western blot印记杂交和细胞免 疫化学法检测其对mRNA和蛋白表达的干涉效果。结果:经酶切鉴定及基因测序证实,重组质粒中已插入目的基因片段。 RT-PCR显示两个干涉载体均抑制了目的基因的转录;Western blot印记杂交和细胞免疫化学检测结果显示pSUPER-S1对蛋 白表达的抑制作用更强。结论:成功地构建了针对人survivin基因的RNA干涉真核表达载体pSUPER-S1和pSUPER-S2,并在 人乳腺癌细胞株SKBR-3中有效发挥了对survivin基因的干涉作用。

 
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