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基因操作技术
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  gene operation
     However, in recent years technical development of molecular biology, people can modify antibody with the gene operation technique to making it be applicable to the paroxysm treatment.
     随着分子生物学技术的发展及其向各学科的渗透,通过基因操作技术对抗体进行改造,可使其适用于多种疾病的治疗。
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  “基因操作技术”译为未确定词的双语例句
     Methods The hγ gene regulated by major late promoter (MLP) was cloned into the upstream of E1A of plasmid PXC70-HRE-TP,which E1A gene and E1B gene were driven by telomerase reverse transcriptase promoter and hypoxia response promoter respectively to produce plasmid pSG500-hγ.
     方法通过基因操作技术将主要晚期启动子(MLP)调控的人干扰素-γ基因插入腺病毒E1A基因受hTERT启动子调控、E1B基因受HRE启动子调控的增殖病毒载体质粒PXC70-HRE-TP的E1A上游,得到腺病毒质粒pSG500-hγ。
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     Using reverse genetics system ,we rescued the four viruses successfμlly, RGSGD/1/96,RGSGD/2/96,RGD/1/2NS,RGD/2/1NS,and infected Chicken embryo fibroblastes cell in order to induce interferon ,Then the green fluorescence protein-vesicμlar stomatis virus (VSV-GFP)infected Chicken embryo fibroblasts cells treated by induced interferon .
     将利用反向基因操作技术救获的四株病毒RGSGD/1/96、RGSGD/2/96、RGD/1/2NS、RGD/2/1NS感染鸡胚成纤维细胞诱生干扰素,然后用插入荧光蛋白的水泡性口炎病毒(VSV-GFP)检测干扰素的含量。
短句来源
     In order to study the influence of NS gene on virulence of H5N1 influenza virus,two virus strains RGSGD/1/96 and RGSGD/1/2NS,each contains a different NS gene,were rescued from plasmids through reverse genetics. The viruses were used to inoculate nasally SPF chickens to compare their pathogenicity.
     为了解H5N1亚型禽流感病毒经自然途径感染SPF鸡后,病毒的致病能力与NS基因的关系,本文主要从病理学角度比较了两株利用反向基因操作技术拯救的病毒RGSGD/1/96和RGSGD/1/2NS的致病能力。
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     In this paper, we summarized the progress in molecular biology of yeast and its application, mainly including the genome, gene expression characteristics of saccharomyces cerevisiae, and its application in molecular biology study and so on.
     对酵母分子生物学从基础理论到在食品发酵工业中应用的研究进展进行了综述 ,阐述了酿洒酵母的基因组、基因表达特点以及基因操作技术 ,对酵母在未来分子生物学研究中的作用以及基因工程改良酵母的工业应用作了展望
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     Technical Improvements in Genetic Manipulation of Pichia pastoris and Their Application in Hirudin Expression
     毕赤酵母基因操作技术的改进及其在水蛭素表达中的应用
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     4 new genes were obtained.
     U基因
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     Genetic Information Carrier Gene
     基因简史
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     Technical Improvements in Genetic Manipulation of Pichia pastoris and Their Application in Hirudin Expression
     毕赤酵母基因操作技术的改进及其在水蛭素表达中的应用
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     Recombineering is a new developed genetic engineering technique in recent years.
     Recombineering(重组工程)是近年来兴起的一项新型基因操作技术
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     Technology Analysis on Decompression Operation
     减压操作技术分析
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  gene manipulation techniques
Gene manipulation techniques open up the possibility of making recombinant human serum albumin (rHSA) or mutants with desirable therapeutic properties and for protein fusion products.
      
Future studies utilizing transgenic animals or gene manipulation techniques will likely answer this question.
      
The structural basis of the enzyme activation mechanism of CaM has been studied using gene manipulation techniques.
      


Surface display is a newly developed gene manipulation technology by which fragments of foreign peptides are expressed as fusion proteins displayed on the phage or cell surface, keeping their spatial structure and biological activity relatively independent. This technology may be used for studying the properties, mutual recognition and interaction of peptides (proteins), so as to survey very large display libraries for target functional peptides. There are three display systems, including filamentous bacteriophage,...

Surface display is a newly developed gene manipulation technology by which fragments of foreign peptides are expressed as fusion proteins displayed on the phage or cell surface, keeping their spatial structure and biological activity relatively independent. This technology may be used for studying the properties, mutual recognition and interaction of peptides (proteins), so as to survey very large display libraries for target functional peptides. There are three display systems, including filamentous bacteriophage, T 4 and T 7 phage,and cell surface systems, mosthy used so far. Display librariesinclude recombinant phage antibody libraries, random short peptide libraries, peptide comformation libraries, cDNA display libraries, and gene mutant display libraries. The surface display technology can be used for the production of artificial antibody and vaccine, the determination of antigen epitopes and protein interactive sites, the isolation of regulatory molecules, the study of cell surface engineering, the development of peptide drugs, and the experimentally directed evolution of biomelecules.

表面展示是一种新的基因操作技术,它使表达的多肽以融合蛋白形式展现在噬菌体或细胞表面,保持相对独立的空间结构和生物活性。该技术可用于研究多肽(蛋白质)的性质、相互识别和作用,并据此从巨大展示库中选择特定靶功能的多肽结构。常用丝状噬菌体、T4噬菌体、λ噬菌体以及细胞构建表面展示系统。表面展示库包括重组噬菌体抗体库、随机短肽库、多肽构象库、cDNA展示库和基因突变体展示库。表面展示技术可用于人工抗体和疫苗的制备、抗原决定簇的定位、蛋白质相互作用位点的确定、特异调节分子的分离、细胞表面工程的研究、多肽药物的研制,以及生物分子实验定向进化等研究

Biotechnology has long been used in treating poluted and waste water. The utilization of genetic engineering to oonstruct a new microorgahism that can decomope special com pound is stride forward. The gene operating technoogy has been used to improve the level of some distinctive enzymes that can decomope me special chendcal oompound in dricrobial cells. Microorganisrns have been made as biological sensor for environmental monitoring.Water purification can be carried out according to the characters of whcroorganisrns,...

Biotechnology has long been used in treating poluted and waste water. The utilization of genetic engineering to oonstruct a new microorgahism that can decomope special com pound is stride forward. The gene operating technoogy has been used to improve the level of some distinctive enzymes that can decomope me special chendcal oompound in dricrobial cells. Microorganisrns have been made as biological sensor for environmental monitoring.Water purification can be carried out according to the characters of whcroorganisrns, which can be arnmnized, vulcanized, and nitrified etc. The utilization of some whcroorganisrns to synthesize some special material to replace plastics and other hard - to - decommp materials has achieved a remarkable results.

在污水和废水处理等坏境净化领域采用生物技术已久,基因工程在此领域的应用正朝着构建能降解特殊化合物的微生物方向迈进;基因操作技术被用来提高某些微生物体内特异性酶类水平,而这些酶具有特异性生物降解转化作用。微生物已在海洋环境保护中被制作生物传感器等用来进行坏境检测;利用微生物的氨化、硫化、硝化等生化特性进行水质净化;利用某些微生物合成特殊物质来代替塑料等难降解物质并在这些领域中获得显著成效。

Trichoderma ia an important group in the biological control of plant diseases,and is ubiquitous in the environment.As a biocontrol agent,the advantages of Trichoderma lie in its excellent adaptability,broad spectrum of activity and diversified mechanisms. T.richoderma spp.with biocontrol activity can be screened directly from the natural environment,however,techniques aimed at improving these agents including mutation,protoplast fusion and gene manipulation have been established.Advances have been achieved...

Trichoderma ia an important group in the biological control of plant diseases,and is ubiquitous in the environment.As a biocontrol agent,the advantages of Trichoderma lie in its excellent adaptability,broad spectrum of activity and diversified mechanisms. T.richoderma spp.with biocontrol activity can be screened directly from the natural environment,however,techniques aimed at improving these agents including mutation,protoplast fusion and gene manipulation have been established.Advances have been achieved in mass production and formulation development.More than 10 registered biocontrol products worldwide are Trichoderma based.Improving screening technique is an important research area,and the progress will depend largely on the full understanding of ecology and mechanisms active in situ. Trichoderma spp.will contribute more to plant disease management in the future.

木霉菌是重要的植物病害防治菌,广泛分布于自然界。木霉菌具有广泛的适应性,能够杀伤多种重要的植物病原菌,作用机制多种多样。除了从自然界分离筛选之外,木霉菌的诱变育种改良技术,原生质体融合技术以及基因操作技术已有效地应用于木霉菌的改造。木霉菌的大量生产技术以及菌剂的制备技术也有较大发展,已经有10 多种商品化制剂。木霉菌的筛选技术是需要重视的研究领域,而其发展则离不开对生态学和作用机制的详细了解。木霉菌具有广阔的研究与应用前景。

 
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