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  genbank number
     The complete sequence(768 bp) of eIF5a3(GenBank number: DQ167203) was obtained using GeneRacer kit.
     利用GeneRace方法得到eIF5a3(基因登录号:DQ167203)的全长为768 bp。
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  “基因登录号”译为未确定词的双语例句
     Two full-length DNA-A molecules (Hn2-1; Hn2-19) from Ageratum conyzoides sample Hn2 were cloned and sequenced. Sequence analyses and comparisons showed DNA-A of Hn2-l and Hn2-19 was most closely related with Ageratum yellow vein virus (AYVV, X74516) (85% sequence identity).
     从胜红蓟样本Hn2中克隆并测定了两个DNA-A全序列(Hn2-1和Hn2-19),经NCBI检索和序列比较分析发现,Hn2-1除与胜红蓟黄脉病毒(AYVV,基因登录号X74516)有85.6%的同源性外,其余均在85.0%以下。
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     3. A photoperiod related gene was cloned using RT-PCR and was named as EMF-like gene (Accession number: DQ343238).
     3.采用同源克隆法,利用RT-PCR技术克隆到玉米上的EMF同源基因,命名为类EMF基因(基因登录号:DQ343238)。
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     OBJECTIVE To study the biological specialties of protein coded by Araneus Ventricousus cathepsin B-like gene Avg 1(genbank AY 302573).
     [目的]研究大腹园蛛组织蛋白酶B相关基因Avg 1(基因登录号:AY302573)编码蛋白的生物学特性.
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     ZLDE-2 was a new member of soybean LEA gene family, and the GenBank accession number was AY351918. They were expressed in pET-28a(+) system, respectively.
     其中ZLDE-2已在GenBank登录,基因登录号为AY351918,它属于LEA基因家族的新成员。
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     Results A novel complete cDNA of thyroid hormone-response protein-1 (TRP-1) gene is cloned. It is 973 bp in f ull-length (Gene Bank accession no. AF348365), and its transcription was enhanc ed in cerebral cortex in neonatal hypothyroidism rats.
     结果 克隆了甲状腺激素反应蛋白 1(thyroidhormone responseprotein 1,TRP 1)基因全长cDNA ,共 973bp(基因登录号为AF3 4 83 65) ,在甲减新生大鼠的脑中 ,该基因的转录增强。
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  相似匹配句对
     4 new genes were obtained.
     U基因
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     Its Gene Rank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property.
     该基因GeneBank登录号为AF458071。
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     The sequence was accepted and released by GenBank(Accession number AY899298).
     该基因在GenBank中登录号为AY899298。
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     Overexpression of AK fbr gene in C.
     AKfbr基因在C.
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  genbank number
Hybridization probes and primers were synthesized in accordance with the reported sequence of theNocardia lat gene from GenBank (number: G1 49355).
      
It is recognized as identical to that found in the other Mediterranean localities (GenBank number AJ228960).
      
Host is indicated along with the isolate or GenBank number when a species name is not available.
      
The number of nucleotides analyzed and the corresponding GenBank number are to the right of the sequence.
      


Objective: To search for new senescence associated gene. Methods: RAPD was used to screen the new senescence associated DNA fragment. Southern blot was used to study gene station and Northern blot was applied to analyze the mRNA level. Results: The 894bp DNA fragment, which was visible only in senescent 2BS cells, was found and named senescence associated DNA (sad). No sad homologous sequences were found in updated GenBank. GenBank accession number of sad was AQ324104. Southern blot showed that sad gene might...

Objective: To search for new senescence associated gene. Methods: RAPD was used to screen the new senescence associated DNA fragment. Southern blot was used to study gene station and Northern blot was applied to analyze the mRNA level. Results: The 894bp DNA fragment, which was visible only in senescent 2BS cells, was found and named senescence associated DNA (sad). No sad homologous sequences were found in updated GenBank. GenBank accession number of sad was AQ324104. Southern blot showed that sad gene might be a one copied gene. Southern patterns of sad gene in senescent 2BS cells were different from that in young cells, but similar to patterns in BGC 823 cells. Northern blot showed sad was expressive in 2BS cells. The size of sad RNA was 0.5kb. The content of sad RNA in young 2BS cells was not different from that of the population doubling 25 to 56. Conclusion: sad was a new molecular marker linked to senescence associated gene. The sad gene may be a one copied gene, which expressed in 2BS cells.

目的 :探索衰老相关新基因。方法 :采用RAPD法筛选衰老相关的DNA片段 ,采用Southernblot分析基因状态 ,Northernblot分析mRNA水平。结果 :筛选出衰老特异的DNA片段 ,长 894bp ,命名为sad。将sad片段克隆、测序 ,查询GenBank ,确认为一种新的衰老相关序列 ,基因登录号为AQ32 410 4。Southernblot分析表明 ,sad基因可能是一种单拷贝基因 ;sad在衰老 2BS细胞中的Southernblot图谱不同于年轻细胞 ,而与人胃癌细胞系BGC 82 3类似。Northernblot分析显示 ,sad基因具有表达活性 ,其RNA长约 0 .5kb ,相对水平在各代龄的细胞间差异无显著性。结论 :sad是一种新的与衰老相关的DNA片段 ,sad基因可能为单拷贝基因 ,具有表达活性。

Objective To is ol ate novel thyroid hormone-response genes, to study the characterizations of the ir expressions and to predict their possible functions in neonatal rats. Methods A neonatal rat model with congenital hypothyr oidism was established and cDNA fragments of novel thyroid hormone-response gen es from cerebral cortex of neonatal rats were obtained by fluorescence-labeled DD-PCR analysis, subcloning and sequencing. Complete cDNAs of novel thyroid hor mone-response genes were cloned by the techniques...

Objective To is ol ate novel thyroid hormone-response genes, to study the characterizations of the ir expressions and to predict their possible functions in neonatal rats. Methods A neonatal rat model with congenital hypothyr oidism was established and cDNA fragments of novel thyroid hormone-response gen es from cerebral cortex of neonatal rats were obtained by fluorescence-labeled DD-PCR analysis, subcloning and sequencing. Complete cDNAs of novel thyroid hor mone-response genes were cloned by the techniques of electronic clone, RT-PCR and sequencing, their expressions regulated by thyroid hormone were confirmed b y Northern blot analysis, their distributions, transcription levels in different tissues and different brain areas were further observed by semiquantitative RT -PCR analysis, and their possible functions were postulated through bioinformat ic techniques. Results A novel complete cDNA of thyroid hormone-response protein-1 (TRP-1) gene is cloned. It is 973 bp in f ull-length (Gene Bank accession no. AF348365), and its transcription was enhanc ed in cerebral cortex in neonatal hypothyroidism rats. The expression of its mRN A was very extensive, but more abundant in brain. Its transcriptional level in d ifferent brain areas was not uniform, much higher in olfactory bulb. Its encodin g protein had some significant domains and motifs. Conclusion TRP-1 gene is a new thyroid hormone-response gene and may play an important role during normal brain development. Its abnormal expression may b e partially responsible for neurological defects in brain arising from thyroid h ormone deficiency during critical period for perinatal rats.

目的 克隆新的大鼠脑甲状腺激素反应基因 ,研究其表达特征并初步预测其功能。方法建立先天性甲状腺功能减退 (甲减 )新生大鼠模型 ,用荧光标记的差异显示PCR(DD PCR)技术、亚克隆、测序及相似性检索获得未知的大鼠脑甲状腺激素反应基因的cDNA片段。采用电子克隆、RT PCR、测序并克隆其cDNA全长。经Northern印迹法证实其表达受到甲状腺激素的调节 ,并用半定量RT PCR方法和生物信息学技术观察其分布转录水平和预测其功能。结果 克隆了甲状腺激素反应蛋白 1(thyroidhormone responseprotein 1,TRP 1)基因全长cDNA ,共 973bp(基因登录号为AF3 4 83 65) ,在甲减新生大鼠的脑中 ,该基因的转录增强。该基因表达广泛 ,以脑组织中的表达水平最高 ;其在大鼠不同脑区的表达含量亦不同 ,以嗅脑最高。该基因的编码产物具有一些重要的结构域和功能基序。结论 TRP 1基因是新发现的甲状腺激素反应基因 ,它在正常的脑发育中可能具有重要的作用 ,其异常表达可能是甲减性脑发育障碍发生的分子机制之一。

The genomic DNA was extracted from wheat cultivars using CTAB method. Based on the known LMW-GS gene sequences reported in GenBank, the primer 1-7 for specific chromosome locus LMW-GS genes were designed and synthetized. Using of the genomic DNA from special wheat germplasm, including hexapliod wheat Abbozanida disome,1A, 1B and 1D nullisomes of Abbozanida,diploid and tetraploid wheat as template, amplified specific locus LMW-GS genes under the optimal reaction system to test these primers. The results showed...

The genomic DNA was extracted from wheat cultivars using CTAB method. Based on the known LMW-GS gene sequences reported in GenBank, the primer 1-7 for specific chromosome locus LMW-GS genes were designed and synthetized. Using of the genomic DNA from special wheat germplasm, including hexapliod wheat Abbozanida disome,1A, 1B and 1D nullisomes of Abbozanida,diploid and tetraploid wheat as template, amplified specific locus LMW-GS genes under the optimal reaction system to test these primers. The results showed that, Primer 3 and 4 were the specific for LMW-GS genes at Glu-D3 locus in wheat,its cycling reaction profile was: 1 min at 94℃, 1 min at 62℃,2 min at 72℃. The size of the PCR product was about 1.60 kb, including promoter and the whole CDS. While primer 5 and 7 were the specific for the LMW-GS genes at Glu-B3 locus in wheat,its cycling reaction profile was: 1 min at 94℃ ,1 min at 64℃,2 min at 72℃. The size of the PCR product was about 1.45 kb, including promoter and the whole CDS. Moreover, LMW-GS coden by cloned gene at Glu-D3 locus of Xiaoyan No.6 contained 9 Cys residues. It was the first time to find a gene of LMW-GS with 9 Cys residues. This may be one of the major reasons that Xiaoyan No.6 has good processing quality.

用CTAB法提取小麦基因组DNA ,根据GenBank中公布的已知LMW GS基因序列 ,设计并合成染色体位点特异PCR引物 1~ 7;利用特殊小麦材料———六倍体普通小麦阿勃二体、1A、1B和 1D缺体 ,四倍体小麦及二倍体的一粒小麦和节节麦的基因组DNA为模板 ,在优化的PCR体系下进行特异性扩增和引物验证。结果表明 :引物 3和引物 4为小麦谷蛋白Glu D3位点LMW GS基因特异PCR引物 ,用其进行扩增时 ,循环反应条件为 :94℃变性 1min ,6 2℃退火 1min ,72℃延伸 2min。扩增产物大小约 1.6 0kb ,包括了启动子和完整编码区。引物 5和 7为小麦谷蛋白Glu B3位点LMW GS基因特异PCR引物 ,用其进行扩增时 ,循环条件为 :94℃变性 1min ,6 4℃退火 1min ,72℃延伸 2min。扩增产物大小约 1.4 5kb左右 ,包括了启动子和完整编码区。此外 ,用引物 3和 4通过PCR技术克隆到小偃 6号小麦Glu D3位点LMW GS基因(登录号为AY2 6 336 9) ;该基因编码的LMW GS含 9个Cys残基。这是首次发现含 9个Cys残基的L...

用CTAB法提取小麦基因组DNA ,根据GenBank中公布的已知LMW GS基因序列 ,设计并合成染色体位点特异PCR引物 1~ 7;利用特殊小麦材料———六倍体普通小麦阿勃二体、1A、1B和 1D缺体 ,四倍体小麦及二倍体的一粒小麦和节节麦的基因组DNA为模板 ,在优化的PCR体系下进行特异性扩增和引物验证。结果表明 :引物 3和引物 4为小麦谷蛋白Glu D3位点LMW GS基因特异PCR引物 ,用其进行扩增时 ,循环反应条件为 :94℃变性 1min ,6 2℃退火 1min ,72℃延伸 2min。扩增产物大小约 1.6 0kb ,包括了启动子和完整编码区。引物 5和 7为小麦谷蛋白Glu B3位点LMW GS基因特异PCR引物 ,用其进行扩增时 ,循环条件为 :94℃变性 1min ,6 4℃退火 1min ,72℃延伸 2min。扩增产物大小约 1.4 5kb左右 ,包括了启动子和完整编码区。此外 ,用引物 3和 4通过PCR技术克隆到小偃 6号小麦Glu D3位点LMW GS基因(登录号为AY2 6 336 9) ;该基因编码的LMW GS含 9个Cys残基。这是首次发现含 9个Cys残基的LMW GS基因。它可能是小偃 6号加工品质优良的主要原因之一。

 
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