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提高酶活
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  increase enzyme activity
     Optimization of the solid substrate and culture conditions was a very important measure to decrease production cost,increase enzyme activity and realize industrial production of cellulase.
     培养基及培养条件的优化是降低酶制剂成本、提高酶活、实现其工业化生产的重要措施。
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     Mg2+, Fe2+, Cu2+, Zn2+ have no evident effection on the enzyme activity;
     eaZ+能显著提高酶活,Mg2+、Fe3+、cu2+、zn2+对酶活作用不明显;
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     One mmol. L~(-1) Fe~(2+) and Ca~(2+)increased the activity by 28% and 14.1%, respectively.
     1mmol L~(-1)浓度的Fe~(2+)和Ca~(2+)有促进酶活作用,分别提高酶活28%和14.1%;
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     In addition,the properties of deaminase were studied,the optimal temperature and pH being 40 ℃ and 5.8 respectively. Enzyme activity was remarkably enhanced in the presence of volume frection 0.2% Tween-80 and 10~(-5) mol/L K~+.
     同时对苯丙氨酸脱氨酶的性质进行了研究,结果表明:该酶最适pH=5.8,最适温度为40℃,反应液中添加φ(吐温-80)=0.2%和c(K+)=10-5mol/L能明显提高酶活
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     By response surface experiment, it was found that the combination use of 0.54% PEG6000, 0.62% trehalose and 0.31% DTT could effectively improve the PAL activity by 92.9%.
     响应面实验发现,海藻糖、聚乙二醇6000、二巯基苏糖醇在实验浓度范围内交互作用很小,0.62%海藻糖、0.54%聚乙二醇6000和0.31%巯基苏糖醇的组合可提高酶活92.9%。
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     (2) enzyme activity of XR can be enhanced by K + in suitable concentration, but it will be inhibited by Mg 2+ ;
     ( 2 )适当的 K+能够提高酶活 ,而 Mg2 +对 XR有抑制作用 .
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     Through the temperature-changing fermentation, the aminoacylase production increased by 13%.
     通过变温发酵,提高了13%。
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     Conclusion Physical mutagenesis can enhance the activity of elastase.
     结论物理诱变可以有效提高弹性蛋白
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     However, activity raised a litter after the complex induced mutagenesin by UV and LiCl.
     随后用紫外线和LiCl复合诱变提高不大。
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     Applying this two-stage agitation rate control strategy, E_(1max) and E_(2max) could be improved by 5.3% and 15.1%, (?) _(1max) and (?)
     PGL和PMGL分别提高5.3%和15.1%;
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     Its enzyme activity is increased 258%.
     该菌株的比原始菌株提高了258%。
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  increase enzyme activity
In the amended soil, the composts did not increase enzyme activity in an additive way.
      
The amendment of humus peat origin (only humic acids) did not increase enzyme activity or inorganic N concentrations of soil.
      
It could increase enzyme activity leading to raised levels of citrullinated protein and stimulating autoantibody.
      
Dietary factors such as fish oil may induce EPHX1 and thus increase enzyme activity,73 and such phenotypic determinants may vary across populations.
      


Peroxidase activity in the abscission zone of explant of Euonymus japoncia Thumb. increases graduelly in the process of natural abscission. For normally growally growing abscission zone, two major bands were observed on its peroxidase isozyme, and four new bands appeared after 72hrs of natural abscission. Peroxidase activity in abscission zone and abscission of explant increased after treatment of 10ppm ethylene or 10ppm ABA. Both the accelerated abscission and peroxidase activity enhanced by ethylene or ABA...

Peroxidase activity in the abscission zone of explant of Euonymus japoncia Thumb. increases graduelly in the process of natural abscission. For normally growally growing abscission zone, two major bands were observed on its peroxidase isozyme, and four new bands appeared after 72hrs of natural abscission. Peroxidase activity in abscission zone and abscission of explant increased after treatment of 10ppm ethylene or 10ppm ABA. Both the accelerated abscission and peroxidase activity enhanced by ethylene or ABA was inhibited by cycloheximide. It is proved that peroxidase is synthesised de nove during the course of induced abscission. The explant treated by ethylene or ABA has a differently changed peroxidase isozyme. After 48hrs of ABA treatment, three new bonds appeared, while no new band was observed after ethylene treatment. ABA stimulated ethylene production and that is one of the possible reason that ABA promoted abscission.

本文以大叶黄杨茎段外植体作材料,研究了自然脱落和乙烯、ABA诱导叶柄脱落过程中过氧化物酶活性和同工酶谱的变化。自然脱落的叶柄在陈化时过氧化物酶活性逐渐增加,到72小时达最高值。乙烯和ABA在加速脱落的同时提高了酶活。用CHI处理减弱了上述两种激素脱落效应的同时降低了酶活。离区吲哚乙酸氧化酶的酶活与过氧化物酶呈正相关性。自然脱落与ABA处理的叶柄离区,其过氧化物酶同工酶有新谱带出现,而经乙烯处理过的却没有明显的谱带变化。文章就激素与有关酶类的控制与被控制的关系从而调节脱落的观点进行了讨论。

In the present paper the effect of divalent cations (Mg2+, Ca2+, Sr2+, Ba2+, Mn2+) on the lipid fluidity, the surface charge density and the activity of H+-ATPase-containing proteoliposomes as well as their interrelationships has been stu died and compared.The results showed that all of th edivalent cations under investigation could decrease both the fluidity and the surface negatively charge density of the proteoliposomes, and increase obviously its ATPase activity. Moreover, it has been demonstrated that(i)...

In the present paper the effect of divalent cations (Mg2+, Ca2+, Sr2+, Ba2+, Mn2+) on the lipid fluidity, the surface charge density and the activity of H+-ATPase-containing proteoliposomes as well as their interrelationships has been stu died and compared.The results showed that all of th edivalent cations under investigation could decrease both the fluidity and the surface negatively charge density of the proteoliposomes, and increase obviously its ATPase activity. Moreover, it has been demonstrated that(i) increase in Mg2+ concentration from 0 to 1.7mM will lead a linear decrease of both the fluidity and the surface negatively charge density of the proteoliposomes, (i i) there is a linear relationship between the changes in the fluidity and the surface charge density, (i i i) it seems that the maximal increase in the reconstituted H + -ATPase activity is correlated with an optimal fluidity of phospholi-pid molecules in proteoliposomes.It was found that divalent cations under investigation enhanced the reconstituted H+-ATPase was a non-competitive activation, it did not affect the combination of the ATP with the enzyme (Km) , but affects only V.The effectiveness of decreasing the lipid fluidity of the proteoliposomes so as to give the maximal activity ranks in the following order. Sr2+>Ba2+≈Ca2+>Mg2+. Butt he effect of Me2+ on the H+-ATPase reconstitution with soybean phospholipid liposomes was not strictly specific.The above mentioned results might provide further support for the hypothesis that divalent cations may play a role in altering the fluidity of phospholipid molecules in proteoliposomes, which would favor the formation of a conformation of the reconstituted H+-ATPase with higher activity.

本文对猪心线粒体H~+-ATP酶在大豆磷脂脂质体上重建过程中,二价金属离子(M_e~(2+))影响膜脂流动性,面电荷密度,酶活及其相关性进行了研究和比较.结果表明:二价金属离子通过与脂酶体酸性磷脂的相作用,影响膜脂的流动性,进而影响H~+ —ATP酶的构象,提高了酶活.

The characteristics of the protease produced by BT--37, a Bacillus thuringiensis isolated from soil, were studied. Some characteristics were as follows: optimal pH 7.0, stable in the range of pH6.0—10.6; optimal temperature 50℃, about 70% of its activity loss at 60℃ for 1.5hr. The protease was strongly inhibited by EDTA(1.5×10~(-3)M) and metal ion Mn could increase the activity by 37%. incubated on PB medium at 30℃. 250rpm for 36hr. When spore and crystal falled off, the peak of the enzyme producing was obtained....

The characteristics of the protease produced by BT--37, a Bacillus thuringiensis isolated from soil, were studied. Some characteristics were as follows: optimal pH 7.0, stable in the range of pH6.0—10.6; optimal temperature 50℃, about 70% of its activity loss at 60℃ for 1.5hr. The protease was strongly inhibited by EDTA(1.5×10~(-3)M) and metal ion Mn could increase the activity by 37%. incubated on PB medium at 30℃. 250rpm for 36hr. When spore and crystal falled off, the peak of the enzyme producing was obtained. Higher toxicity production was obtained in the medium with higher protease activity.

BT—37是从土壤中分离出的一株对多种鳞翅目昆虫有较高毒力的苏芸金芽孢杆菌。在PB培养基中振荡培养,蛋白酶活性随着细胞的生长而增强,培养36小时,芽孢晶体开始脱落时达到高峰。所产蛋白酶为中性蛋白酶。酶作用的最适条件:最适温度为50℃,60℃保温1.5小时酶活性降低70%;最适pH7.0;在pH6.0℃—10.6范围内稳定。1.5×10~3M时,Mn~+显著提高酶活。在蛋白酶活性高的培养基中获得了较高毒力的培养物。

 
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