Methods Eight pairs of primers designed from exon and intron sequence of the RP2 gene were used for the amplification of eight segments which encompass all exons of the gene. PCR were carried out with human genomic DNA as the template.
By generlizing parameter HI ,the interference index R is defined. The character of several sequence segments of exon and intron is studied by use of R, and the relation between the distribution of R and the number of sequence segments of exon and intron is gained.
通过推广 H I参数 ,定义干涉指标 R,对外显子和内含子的多个片段的特征进行研究 ,得到了 R值分布以及与外显子和内含子片段的关系 .
Bioinformatics analysis shows that there are a lot of potential cis-acting elements,including some enhancers in the NGAL gene (X99133, including exon and intron).
Methods: A total of 14 pairs of primers designed from exon and intron sequences of the Parkin gene were used for the amplification. PCR was carried out with human genomic DNA as the template.
Methods We selected 4 target mRNA sequences to the exon and interon of HPV-16 E6 mRNA, and synthesized dsDNA to construct the recombinant pSi-lencer1.0-U6 vector which expresses HPV-16 E6 short hairpin dsRNA. After transfected into HPV-16 DNA positive cervical cancer cell line CaSki, then observe the expression level of HPV-16 E6,E7 genes and their proteins as well as the inhibition effects of cell growth.
Calculations of the exon/intron numbers and the lengths of exon and intron revealed that all of the rice CDPK genes had the longest exon at the position of exon 1, but the lengths of introns in different genes showed different patterns.
Levels of nucleotide diversity for both the exon and intron sequences were greater among than within species, and there were numerous species-specific nucleotides present in both the coding and noncoding regions.
This suggested that generation of the repetitive sequences spanning the exon and intron regions had occurred before the diversification of these species.