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细胞病毒
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  cell viruses
     Methods The murine stem cell viruses which were recombinated with parathyroid hormone (PTH) gene were harvested. TC-1 were infected with the recombinant retroviruses of 1 ml with addition of polybrene (final concentration:8 mg/L). The gene transduction efficiency in TC-1 was assayed and PTH secretion observed.
     方法包装出重组人甲状旁腺素 (PTH )基因的小鼠干细胞病毒 (MSCV) ,以lml重组病毒液加入 poly brene(终浓度 8mg/L)感染TC 1细胞 ,检测基因转导效率 ,PTH分泌情况 ;
短句来源
     Methods The murine stem cell viruses which recombinated with parathyroid hormone (PTH) gene were harvested. The recombinant retroviruses were titred and used to infect TC-1.The gene transduction efficiency in TC-1 was assayed and the differentiation in vivo and in vitro observed.
     方法包装出重组人甲状旁腺素 (PTH )基因的小鼠干细胞病毒 (MSCV) ,以其感染小鼠ESCs ,检测基因转导效率 ,PTH分泌情况 ;
短句来源
  cell viral
     With molecular cloning technique, the recombinant stem cell viral vector with report gene was constructed, which is that MSCV-BDNF-IRES_2-EGFP vector could encode Polycistronic mRNA. Viral particle was packaged by PT67 cell line and infected neural stem cells (mouse clone:C17.2).
     采用分子克隆技术构建带有绿色荧光蛋白报告基因的多顺反子的MSCVBDNFIRES2EGFP重组鼠干细胞病毒表达载体,病毒经PT67细胞包装后感染神经干细胞株C17.2。
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  “细胞病毒”译为未确定词的双语例句
     Vitamin D 3 Inhibits the Expression of Viral Early Genes in HCE16/3 Cells in Combination With Retinoic Acid
     维生素D_3和维甲酸合并使用抑制HCE16/3细胞病毒早期基因表达的研究
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     Methods The effect of AEVL on Raji cell early antigen (EA) expression and B 95-8 cell virus capsid antigen (VCA) expression was assayed by indirect fluorescent technique;
     方法 用间接荧光法测定抗 EB病毒口服液对 Raji细胞早期抗原 (early antigen,EA)表达及 B95- 8细胞病毒壳抗原 (virus capsid antigen,VCA)表达的影响 ;
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     After antibiotic selection, the resulting cell lines HepG2/Off-VP3 were established. Apoptin gene expression in HepG2/Off-VP3 cells was controlled by the presence or the absence of tetracycline in medium.
     取PT67/Off和PT67/VP3细胞病毒上清液依次感染人肝癌细胞系HepG2,用抗生素筛选出抗性细胞株HepG2/Off-VP3。
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     Investigation of serum human T-cell lymphotropic virus type I antibody in partial donors In Beijing area
     北京地区部分献血员嗜人T细胞病毒I型血清抗体调查
短句来源
     Mothods:The effect of 1,25-D 3 and retinoic acid, alone and in combination, on the expression of viral early genes in cultured HCE16/3 cells was determined by Northern blot.
     方法:使用Northern印迹法分析了维生素D3或(和)维甲酸处理的HCE16/3细胞病毒早期基因表达。
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  相似匹配句对
     Culture of Epidemic Hemorrhagic Fever Virus in Vero-E_6 Cells
     流行性出血热病毒细胞培养
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     Epstein-Barr virus and T cell lymphoma
     EB病毒与T细胞淋巴瘤
短句来源
     ALBUMINOUS CELLS
     蛋白细胞
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     SPLINTER CELL
     分裂细胞
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     sendai virus
     仙台病毒
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  cell viruses
The strategy we describe is of a general nature and thus is applicable to a variety of other prokaryotic and eukaryotic cell viruses.
      
  cell viral
The pattern of heterogeneous cell-to-cell viral spread can be explained by low production of infectious viral particles in only few areas of the cell membrane.
      
However, a direct link between cell viral infection and islet inflammation has not been established.
      
LCM might transform and immortalize bone marrow cells by CSF-1 regulated, cell-surface receptor-facilitated L-cell viral entry into the host cell.
      
This study focused on the production and the potential role of anti-CCR5 autoantibodies in preventing HIV infection and cell-to-cell viral spread.
      


Cytomegalovirus infection usually occurs in children or patients with decreased immunity. Recently, the disease is often seen in previously healthy adults. Four cases of cytomegalovirus infection in previously healthy adults were admitted from September 1977 to January 1978. All were women, 14 to 44 years of age. The temperature was 38℃ to 40℃ on admission with the longest course of eight weeks and the shortest of two weeks. Hepatomagaly was noted in all cases. Liver function tests revealed abnormalities in...

Cytomegalovirus infection usually occurs in children or patients with decreased immunity. Recently, the disease is often seen in previously healthy adults. Four cases of cytomegalovirus infection in previously healthy adults were admitted from September 1977 to January 1978. All were women, 14 to 44 years of age. The temperature was 38℃ to 40℃ on admission with the longest course of eight weeks and the shortest of two weeks. Hepatomagaly was noted in all cases. Liver function tests revealed abnormalities in three. Three of the four cases showed rashes on the face, extremities and trunk. One case had myocarditis with T-wave change on the electrocardiogram, and other one had interstitial pneumonia. Heterophil agglutination tests were done in three cases and found to be normal. The diagnosis was confirmed by the finding of large inclusionbearing-cells in the urine sediment. All four cases recovered after supportive treatment. The prognosis of cytomegalovirus infection in previously healthy adults is usually good.

本文报道和分析了原健康成人巨细胞病毒感染4例的主要临床表现——发热、浅表淋巴结肿大、皮疹、肝炎表现、心肌炎、肺炎等以及一些实验室检查的异常结果,并讨论了本病的诊断依据,重点介绍了尿沉渣找巨细胞包涵体作为一项简而易行的诊断方法。原健康成人罹患本病后,一般预后良好,4例患者经对症治疗后,均热退,全身情况好转而出院.

This paper reports the study on the ELISA indirect method for detection of antibody to HSV as follows: The HSV antigens were prepared from the cells of the primary rabbit kidney, primary chicken embryo. FL and Vero by two simple methods i. e. freezing and thawing the virus-infected cells repeatedly either with maintaining the medium ( Method Ⅰ ) or after being washed with PBS ( Method Ⅲ). It was found that the titer of antigens prepared from Vero cells by method Ⅱ was the highest and that from primarv rabbit...

This paper reports the study on the ELISA indirect method for detection of antibody to HSV as follows: The HSV antigens were prepared from the cells of the primary rabbit kidney, primary chicken embryo. FL and Vero by two simple methods i. e. freezing and thawing the virus-infected cells repeatedly either with maintaining the medium ( Method Ⅰ ) or after being washed with PBS ( Method Ⅲ). It was found that the titer of antigens prepared from Vero cells by method Ⅱ was the highest and that from primarv rabbit kidney cells was lower. Antibody titers in ELISA from 16 selected sera were compred with the antibody titers by the neutralization test and a significant relationship (r=0.79, P<0.005 ) was found between them. The ELISA AA492>0.2 was considered positive. The levels of antibody in three age groups were detected with ELISA. The findings showed that the positive rate of the infant group was 6.6%, of the children group 80% and of the adult group 100%. Finally, the comparison of enzyme-labelled anti-IgG with enzyme-labelled SPA in ELISA for 29 sera was performed, The results have indicated that the value obtained in SPA-ELISA is close to that in anti-IgG ELISA (r=0.986, p<0.0005). It suggests that the use of enzyme labelled SPA is significant in differentiating serum antibodies into HSV type Ⅰ and Ⅱ.

本文对ELISA间接法检测HSV抗体作了下列研究:以两种较简易的方法,即病毒感染细胞连同维持培养基一起(方法一)或经洗涤后在PBS中(方法二)反复冻融,分别从原代兔肾、原代鸡胚、传代Vero和FL细胞制备HSV抗原,结果以方法二制取的Vero细胞病毒抗原滴度最高;原代兔肾者次之。选取16份ELISA呈不同滴度的血清作中和试验,两法结果呈正相关关系(r=0.79,P<0.005)。初步得出△A492≥0.2可作为血清抗体阳性。以ELISA检测3组年龄人群血清抗体,婴儿组抗体阳性率6.6%,儿童组80%,成人组100%。最后,比较了酶联抗人IgG及酶联SPA ELISA检测的29份血清抗体含量,两种结合物测得的结果呈正相关关系(r=0.986,P<0.0005),并提出酶联SPA在鉴别两型HSV血清中具有重要意义。

Reproduction dynamics and cytopathology of CMV on various cells have beenstudied.1. 2BS passaged cells are most sensitive to CMV and CPE appears to be obvious,regular and steady. The result derived from the primary Huelu cell is similar tothe 2BS cell, but it is easily influenced by the individual difference of embryotissue. Though CMV can also reproduce on the vero-passaged cell, it should take28-30 days for showing CPE and it is diffcult to titrate the virus. The cause ofslow CMV reproduction was analysed.2....

Reproduction dynamics and cytopathology of CMV on various cells have beenstudied.1. 2BS passaged cells are most sensitive to CMV and CPE appears to be obvious,regular and steady. The result derived from the primary Huelu cell is similar tothe 2BS cell, but it is easily influenced by the individual difference of embryotissue. Though CMV can also reproduce on the vero-passaged cell, it should take28-30 days for showing CPE and it is diffcult to titrate the virus. The cause ofslow CMV reproduction was analysed.2. The characterisiics of CMV cytopathology can be Summed up as follows: At the primary stage of infection, pyknosis, circulalization take place on thecell. Deep cytoplasm stain and esosiphilic inclusion bodies also occur. Swelling ofthe cell is the major characteristics. At the middle stage, first there appear gathering and joint of vast pathologicalcells on the scattered area and form petterns caused by a variety of lesions. Thelesion is another characteristics of CMV. At late stage the lesion expanded, the amount of cell lesion is increasedand begins to come off. Inclusion bodies and various cytopathological patherus havegreat diagnostic significance It is very rare in viral infection.

研究巨细胞病毒(CMV)在不同细胞上的繁殖规律及细胞致病作用的特点,对于分离、鉴定病毒和抗原制备,为提供临床诊断及流行病学调查是有一定价值的。本文报告了在不同细胞上CMV所致的CPE特点以及CMV感染的细胞病理学观察结果。

 
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