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     With certain deep pit project as example, dangerous slide surfaces of all construction steps are gotten using this method. Safe coefficients of 3 kinds of operating modes including not adding nails, already adding nails of this step and not adding nails of this step, are compared.
     以某深基坑工程为例,利用该方法得到了土钉支护各个施工阶段的危险滑动面,比较了不加钉、本步已加钉和本步尚未加钉3种工况下的安全系数.
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  “已加”译为未确定词的双语例句
     3) Blood taken and preservation after centrifugation: 5ml blood were taken from vein in right upper arm, then blood was added in pre-cold test tube treated with anticoagulant and trasylol.
     3)抽血及血样的离心保存:均于右上肢皮下头静脉抽血snd,立即加人预冷,已加人抗凝剂及抑肽酶的试管中,并低温离心,-20℃保存血浆。
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     Then I purified and extracted the target fragment by using UNIQ-10 pillar purification kit and cloned the product added A-tail into pGEM-T easy vector by T-A ligation. The JM109 competent cells were transformed with the recombinant plasimid. Consequently,the recombinant plasimid was amplified and extracted from the transformants and tested the inserting of DNA fragment.
     用UNIQ-10 柱式DNA 胶回收试剂盒回收纯化目的片段,通过T-A 连接将已加A 尾的PCR 产物连接到线状克隆载体pGEM-T easy vector,转化JM109 感受态细胞,在含氨苄青霉素的LB 平板上筛选阳性克隆。
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     In the present study,one pair of specific primers to NA gene of Nl subtypes were designed to amplied cDNA of Nl by RT-PCR,purified the production of PCR used reagents made by ourself and cloned the production added A-tail into pGEM-T easy vector by T-A ligation.
     用自制的试剂纯化目的片段,通过T-A连接将已加A尾的PCR产物连接到克隆载体PMD 18-T Vector上,转化JM109感受态细胞,在含氨苄青霉素的LB平板(或麦康凯琼脂平板)上筛选阳性克隆,经PCR扩增,进一步确证为目的片段,并对目的片段进行测序。
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     With the mag-card inserting into the fuelling controller,fuelling begins with the refuel-er picked off,fuelling stops with the refueler hung on when tank is full or filled to the need,later, settlement is carried on,and then the mag-card is returned.
     该系统采用磁卡加油,分为非定量加油和定量加油两种方式,把磁卡插入加油控制器,摘下油枪即可加油,当油箱加满或已加至需要量时,挂枪即停止加油,随即进行结算、退卡。
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     It's a challenge and an opportunity for China's agriculture after China's entering WTO.
     我国已加人WTO,这对农业既是挑战又是机遇。
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     When the cDNA was expressed in E.
     该cDNA在E .
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     PRACTICE ON DESIGN OF ADDING NEW FLOOR OF BUILDING
     建房屋层设计的工程实践
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     According to the above results, Rps.
     Rps.
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     Conclusion:The proposed method succeeded in spiked sample determination with simple and sensitive characteristic.
     结论:该方法快速、简便,成功用于标样品的分析。
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     group E, basal ration plus black rice;
     E组黑米;
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A 32P-postlabeling method that labeles adducted bases specifically are established to detect DNA adducts. In this study, DNA is digested with nuclease P1 and prostatic acid phosphatasc. The normal nucleotides are released as normal nucleosides (N), and the adducted nuclcctidcs are excised as adducted dinucleotides (XipN). The dinucleotides (XipN) but not nuclcosides (N) are converted to 5'-32P-labeled dinucleotides ( pXipN), by T4 polynucleotidc kinasc-catalyzed [32P] phosphate transfer from [y-32P] ATP. Then...

A 32P-postlabeling method that labeles adducted bases specifically are established to detect DNA adducts. In this study, DNA is digested with nuclease P1 and prostatic acid phosphatasc. The normal nucleotides are released as normal nucleosides (N), and the adducted nuclcctidcs are excised as adducted dinucleotides (XipN). The dinucleotides (XipN) but not nuclcosides (N) are converted to 5'-32P-labeled dinucleotides ( pXipN), by T4 polynucleotidc kinasc-catalyzed [32P] phosphate transfer from [y-32P] ATP. Then DNA adducts are purified and separated by thin layer chromatography on polyethylencimine (PEI)-ccllulose sheets, and produced characteristic autoradiographic fingerprints. FL cells are treated with different concentration of benzo(a)pyrene for 24 hours, four adduct spots are found and the total RAL has dose-response relationship.

本文报道建立~(32)P后标记方法(~(32)P-Postlabeling Method)以用于检测致癌物-DNA加成物。其基本原理:用核酸酶P1(Nu.P1)完全消化DNA,释出5′单磷酸脱氧核苷(pN),而加成的碱基(Xi),则释出5′磷酸二脱氧核苷(pXipN);经前列腺酸性磷酸酶(PAP)作用后,分别生成N和XipN,然后经T_4多聚核苷酸激酶(PNK)作用,将[γ-~(32)P]ATP的~(32)P转移XipN的5′末端上,生成标记*pXipN而N不被标记。经聚乙烯亚胺(PEI)纤维素薄层纯化、分离和放射自显影,则可确定已加成的碱基,若DNA量已知,则可进行定量分析。在人羊膜上皮细胞FL中,适量苯并(a)芘[B(a)P]处理24b后,可分离到4个加成物斑块,其总相对标记量(Relative Adduct Labeling,RAL)有剂量依赖性关系。

Location of antibiotic resistance genes in S.aureus isolated in Nanjing and Xian by plasmid elimination,transduction,and protoplast-transformation was reported.It was demonstrated that antibiotic resistance genes encoding Pc,Tc,Cm and Em resistance were lcaded on 42 plasmids in 23 strains of S. aureus,and some of genes encoding Pc ,Tc ,Km ,Gm ,Sm ,and Em resistance were loaded on chrcamosomes.A figure of plasmid profiles containing resistance plasmids described above was presented.Molecular weights of Pc plasmids...

Location of antibiotic resistance genes in S.aureus isolated in Nanjing and Xian by plasmid elimination,transduction,and protoplast-transformation was reported.It was demonstrated that antibiotic resistance genes encoding Pc,Tc,Cm and Em resistance were lcaded on 42 plasmids in 23 strains of S. aureus,and some of genes encoding Pc ,Tc ,Km ,Gm ,Sm ,and Em resistance were loaded on chrcamosomes.A figure of plasmid profiles containing resistance plasmids described above was presented.Molecular weights of Pc plasmids ranged from 11.6~18. 4 Md;Tc plasmids,2.8~4. 2 Md;Cm plasmids,1.8~2. 0 Md; Em plasmids,1.5~2. 5 Md.Among them nine representative plasmid clones were obtained by protoplast-transformation or transduction,and their resistance level and sizes were also determined.

自南京及西安各医院分离的金黄色葡萄球菌中通过质粒消除试验、转导及原生质体转化进行了抗生素抗药基因的定位。实验证明在23株菌中,编码青霉素、四环素、氯霉素及红霉素抗药性的基因存在42个抗药质粒上,编码青霉素、四环素、红霉素、卡那霉素、庆大霉素及链霉素的抗药基因存在染色体上。提供了含上述抗药质粒的质粒谱。青霉素抗药质粒的分子量为11.6~18.4Md,四环素抗药质粒为2.8~4.2Md,氯霉素抗药质粒为1.8~2.0Md,红霉素抗药质粒为1.5~2.5Md。从4株代表菌中通过原生质体转化或转导获得9株含单一抗药质粒的克隆,它们所含质粒的大小及抗药水平也已加测定。

Because the settlement method with receipt or cash exists many malpractices in oil product sales,Tianjin Huayuan Group(Tianjin)has successfully developed MICB-810 mag-card automatic fuelling management system which realizes the integration of automatic fuelling, settlement and computer management. This system makes the fuelling operation simple and convenient,and over- comes the malpractices existed in traditional fuelling method. At present, the system has been used in more than 60 fuelling stations which are...

Because the settlement method with receipt or cash exists many malpractices in oil product sales,Tianjin Huayuan Group(Tianjin)has successfully developed MICB-810 mag-card automatic fuelling management system which realizes the integration of automatic fuelling, settlement and computer management. This system makes the fuelling operation simple and convenient,and over- comes the malpractices existed in traditional fuelling method. At present, the system has been used in more than 60 fuelling stations which are in Beijing,Tianjin,Daqing,Qingdao and Jiang-men. The paper introduces the system of the design concept,system components,main functions, features and its application. The system has the two fuelling -ways-non-quantification and quantification. With the mag-card inserting into the fuelling controller,fuelling begins with the refuel-er picked off,fuelling stops with the refueler hung on when tank is full or filled to the need,later, settlement is carried on,and then the mag-card is returned. The design of the open network and multi-management are used,which is wide coverage,so the provincial data interchange can be realized so as to carry on macro-control and management.

鉴于在成品油销售中采用油票或现金加油结算的方法存在许多弊端,华源集团开发了华源MIC-810B型磁卡自动加油管理系统,实现了自动加油、结算和计算机管理一体化,使加油工作简便快捷,克服了传统加油方式弊端。该系统已在北京、天津、大庆、青岛、江门等地的60多个加油站正式启用。介绍了华源MIC-810 B型碰卡自动加油管理系统的设计思想、系统组成、主要功能、特点和推广使用情况。该系统采用磁卡加油,分为非定量加油和定量加油两种方式,把磁卡插入加油控制器,摘下油枪即可加油,当油箱加满或已加至需要量时,挂枪即停止加油,随即进行结算、退卡。采用开放式网络化;设计、多级管理,覆盖面广,可进跨省市地区间数据交换,便于进行宏观调控和管理。

 
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