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制备大量
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  prepared in quantity
     The loading TiO_2 photo catalyst can be prepared in quantity by hydrolyzation of titanium alkoxide, which has use for reference to wastewater treatment.
     利用钛醇盐水解法可以制备大量负载型TiO_2光催化剂,对于实际废水处理有一定的借鉴作用。
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  “制备大量”译为未确定词的双语例句
     In order to produce a large amount of ScFv H12, pET28a-H12 expression vector was constructed and ScFv H12 was expressed at yield about 30% of total proteins in E.
     为了快速制备大量具有生物活性的单链抗体H12,构建了pET28aH12原核高表达载体,表达量占菌体总蛋白质30%以上。
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     Results we get pCMV-Bam- neo-nm23-H1 successfully and establishthe stable expression of nm23-H1.It is showed that 986bp of nm23-H1 is inserted into pCMV-neo-Bam vector by electrophoresis on a 1% agarose geland stained with ethidium bromide.
     结果(1)制备大量nm23-H1真核表达质粒,限制性内切酶酶切证实nm23-H1插入片断长度为986hp。
     Objective: To block the apoptosis of T lymphocytes induced by Fas/FasL in order to establish a method of the large-scale preparation of large amounts of tumor-specific cytoxic T-lymphocytes (CTL).
     目的通过阻断Fas/FasL介导的T细胞凋亡,建立快速制备大量激活的肿瘤特异性细胞毒性T淋巴细胞(CTL)的方法。
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     The process can prepare mass samples in a short time and fit for molecular marker detection of F 2 segregant population and screening of breeding materials.
     该方法可在短时间内制备大量样品 ,适于进行遗传连锁性分析时对F2 分离群体的单株检测和分子标记辅助选择时筛选育种材料 ,操作简单 ,实用性强 ,值得推广。
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     1. By Mini-MACS CD34+ hematopoietic stem cells, present at afrequency of about 0.5% in normal bone marrow, can be rapidly andefficiently enriched to a purity of more than 90%.
     1.采用Mini-MACS分离技术可以从正常人骨髓分离得到高纯度(>90%)的CD34~+造血干/祖细胞,且回收率(90%左右)和活细胞率(>95%)均很高,这种方法为进一步获得较纯净DCs提供了保证,符合临床肿瘤生物治疗时制备大量高纯度DCs的需要,值得推广。
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     This procedure was simple and suitable for large scale preparation.
     此过程比较简单,适合大量制备
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     Abundant preparation and identification of recombinant human Tau protein
     重组人类Tau蛋白的大量制备及鉴定
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     PREPARATION OF METHYL TERTIARY BUTYL ETHER
     甲基叔丁基醚的制备
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     Preparation of acetaldehyde diethyl acetal
     乙醛缩二乙醇的制备
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  prepared in quantity
A third product, a powdered Bovine Muscle candidate reference material has been prepared in quantity.
      


Using the“EA_3-867”cellulase prepared in our laboratory,viable mesophyll protoplasts were isolated from Solanum nigrum L.The protoptasts grew and divided when cultivated in hanging drops and thin liquid layer of NT medium,and calli formed.After transfering the calli on Dudits and MS solid medium(both supplemented with zeatin 1 mg/l,NAA 0.5 mg/l),regenerated plantlets had been obtained.The effects of different inositol amounts in NT medium on the growth of protoplasts were compared.The percentage of 1st and 2nd...

Using the“EA_3-867”cellulase prepared in our laboratory,viable mesophyll protoplasts were isolated from Solanum nigrum L.The protoptasts grew and divided when cultivated in hanging drops and thin liquid layer of NT medium,and calli formed.After transfering the calli on Dudits and MS solid medium(both supplemented with zeatin 1 mg/l,NAA 0.5 mg/l),regenerated plantlets had been obtained.The effects of different inositol amounts in NT medium on the growth of protoplasts were compared.The percentage of 1st and 2nd cell division after 5—8 days culture and the number of cell clusters increased in NT medium containing 250 mg/l inositol.

用自制的纤维素酶从龙葵(Solanum nigrum L.)叶肉细胞制备了大量有活力的原生质体。用悬滴和浅层培养法,龙葵原生质体生长、分裂、形成愈伤组织,并再生成完整的植株。比较了在 NT 培养基中不同肌醇含量对原生质体的影响;每升培养基中加250毫克的肌醇能促进龙葵叶肉原生质体的生长和分裂。

For poly-crystalline semiconductor film deposited on an insulator and then recrystallized by a laser beam, the influence of the substrate upon recrystallization of the film was analyzed theoretically. We found the critical laser power necessary for solid-liquid phase transition to take place, will change to a lower value as the poor thermal conduction of insulating substrate being reasonably considered. It is well known that the existence of stress in recrystallized films may be taken as a criterion for melting...

For poly-crystalline semiconductor film deposited on an insulator and then recrystallized by a laser beam, the influence of the substrate upon recrystallization of the film was analyzed theoretically. We found the critical laser power necessary for solid-liquid phase transition to take place, will change to a lower value as the poor thermal conduction of insulating substrate being reasonably considered. It is well known that the existence of stress in recrystallized films may be taken as a criterion for melting process that undergoes during laser irradiation. A large number of SOI samples prepared by LPCVD technology were studied after its laser recrystallization, to obtain the critical transition conditions through strass determination by use of Raman peak shifts. A comparison of the calculations based on the model taking low thermal conductance of the substrate into account with the experimental results showed its fitting is better than that with the influence of substrate being neglected.

从理论的角度分析了绝缘衬底对其上面半导体多晶膜激光熔化再结晶过程的影响,发现低导热的绝缘层使产生固-液相变的临界激光功率有明显的降低。用喇曼光谱测量了激光再结晶SOI层中的应力。应力的出现是多晶膜内曾经发生过固-液相变的佐证。从这一思想出发,对LPCVD方法制备的大量SOI样品进行激光再结晶临界条件的研究,证明了忽略绝缘层低热导影响的模型不能解释实验结果,而经过修正的公式则可以较好地拟合实验结果。

We had studied the reproduction of swine vesicular disease virus (SVDV) in the micro-cells and the microcytoplasts prepared from IB-RS-2 cells.More than 70% of the micro-cells of IB-RS-2 cells were alive in the nor-mal culture for 60 hours,while the micro-cells infected with SVDV were almost broken away from the substrate in 5 hours after in-fection.The infective progeny virus was de-tected on the infected microcells.

本实验研究了SVDV在微细胞和微胞质体两种非完整细胞体系内的复制。用秋水仙素处理IB-RS-2细胞,在含有松胞素B的Ficoll 400不连续梯度中进行高速离心,制备了大量的微细胞。微细胞在正常的培养条件下60小时后仍有70%以上存活,用SVDV感染只需4—5小时就几乎全部脱落。并证明微细胞能繁殖出有感染力的子代病毒。用松胞素B去核技术制备的微胞质体,运用扫描电镜和显微放射自显影的方法证实了SVDV可以在微胞质体内复制病毒RNA,并引起微胞质体病变。同时纯化了用松胞素B处理悬浮的IB-RS-2细胞而制备的大量的微胞质体,SVDV感染后,可以产生一定数量的有感染力的子代病毒。虽然滴度低于完整细胞繁殖的病毒,但证明了SVDV可以利用微胞质体中少量的细胞成分和酶类完成其感染、复制和装配一系列繁殖过程,产生有感染力的子代病毒。

 
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