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   制备物 的翻译结果: 查询用时:0.273秒
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制备物
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  preparing product
     OBJECTIVE To study the preparation technology of chitohexose that is the main component, and to analyze the component of the preparing product.
     目的 研究以壳六糖为主的壳寡糖制备工艺并对制备物组分进行分析。
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     A ΔnifZ Av1 preparation with high purity of >90% was obtained by the more fine collection on the Sephacryl S-200 and NaCl gradient elution on the Q-Sepharose.
     通过Sephacryl S-200柱洗脱峰收集精度的调整及Q-Sepharose柱的NaCl浓度梯度洗脱,得到了纯度大于90%的ΔnifZ Av1制备物
短句来源
     Through the anaerobic chromatography on the columns of DEAE 52, Q-Sepharose and Sephacryl S-200, the purer ΔnifZ MoFe protein (ΔnifZ Av1) preparation was obtained from the crude extract of a nifZ-deletion mutant of Azotobacter vinelandii (DJ194).
     从棕色固氮菌DJ194菌株得到的固氮酶粗提液经DEAE-52、Sephacryl S-200及Q-Sepharose等柱厌氧层析,分离纯化得到nifZ基因缺失的固氮酶MoFe蛋白(ΔnifZ Av1)制备物
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     The Km of the enzyme was 6.67 mM when xylose was used as the substrate. The enzyme was activated by Mg2+, Co2+ and Mn2+ and inhibited by Zn2+, Cu2+ and Fe3+.
     该酶对底物木糖的Km值为6.67mmol/L,Mg2+、Co2+和Mn2+对该酶有激活作用,而Zn2+、Cu2+和Fe2+对酶有抑制作用。 酶制备物转化木糖为木酮糖产率为18%。
短句来源
     Hybridomas are prepared by the fusion of mouse myeloma cells,cell line P_3-X63-Ag8.653,and spleen cells from BALB/C mice immunized with partially clarified and concentration sap from Citrus tristeza virus(CTV) infected citron.
     以感染枸橼的柑桔速衰病毒(Citrus tristeza virus,CTV)的部分纯化制备物和浓缩的感染组织粗浸出液免疫BALB/C小白鼠后获得的脾脏细胞,与鼠骨髓瘤细胞株P3-X63-Ag8.653融合制备了杂交瘤细胞.
短句来源
     The HP somatic protein showed significant cytotoxic activity to Hela cell, with LD 50 less than 12 μg/ml.
     体外培养中HP菌体蛋白制备物对Hela细胞具有明显的细胞毒活性 ,LD50 小于 12 μg/ml。
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  相似匹配句对
     Preparation of Chitosan and Its Degradation Products
     壳聚糖及其降解制备
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     Preparation on Amorphous Matter of Malotilate
     马洛替酯无晶化制备
短句来源
     PREPARATION OF METHYL TERTIARY BUTYL ETHER
     甲基叔丁基醚的制备
短句来源
     Preparation of acetaldehyde diethyl acetal
     乙醛缩二乙醇的制备
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  preparing product
Conformal Coating, -Part Addition-Curing These figures are intended as a guide and should not be used in preparing product specifications.
      
These figures are intended as a guide and should not be used in preparing product specifications.
      


In a previous report it was shown that the activity of coupling factors isolated from chloroplasts of other plant species was higher than those obtained from chloro- plasts of the same species for restoring the cyclic photophosphorylation activity of the deficient particles.However,the coupling factors then used were crude extracts or extracts after partial purification only.Verification of the results with purified preparation was highly desirable. To this end several methods of extraction and purification...

In a previous report it was shown that the activity of coupling factors isolated from chloroplasts of other plant species was higher than those obtained from chloro- plasts of the same species for restoring the cyclic photophosphorylation activity of the deficient particles.However,the coupling factors then used were crude extracts or extracts after partial purification only.Verification of the results with purified preparation was highly desirable. To this end several methods of extraction and purification of chloroplast coupling factors were compared.It was found that the coupling factors extracted with dilute EDTA solution from the chloroplasts after the removal of heterogenous proteins showed a single band on polyacrylamide gel electrophoresis.When deficient particles from spinach chloroplasts were incubated with coupling factors purified in this way from broad bean (Vicia faba L.) or barley (Hordeum vulgate L.),the restored photo- phosphorylation activity was still higher than that incubated with coupling factors prepared from spinach chloroplasts and purified in the same way. Thus it can be concluded that the enhancement effect of coupling factors from different species was due to the coupling factors per se and not to any substance(s) contaminated in the crude extracts.

前报指出高等植物不同品种间偶联因子互换,光合磷酸化活力的恢复比同种重组的高。但所用的偶联因子是粗提物或部分提纯的制备物。因此,用提纯的偶联因子来检定此效应很有必要。本文比较了几种提取纯化偶联因子的方法,发现叶绿体先经氯化钾稀溶液除去杂蛋白质,再用乙二胺四乙酸(EDTA)稀溶液提取,提取物在聚丙烯酰胺凝胶电泳上表现为单带。菠菜的残缺膜片与用此种方法提纯的蚕豆或大麦偶联因子(CF_1)保温时,恢复的磷酸化活力仍比与菠菜提纯的偶联因子重组活力高。所以我们认为,不同品种间互换偶联因子的增益效应是由于偶联因子本身,而非粗提液中所含的其他物质的作用。

Extracts of cornbread inoculated with fungi isolated from Linxian,Fusali-um moniliforme (FM),or Fusalium mo-niliforme,Aspergillus flavus,Geotrichum can-didum,Penicillium cyclopium (FAGP),in-creased significantly the frequency of sister chromatid exchange in V_(79) cells and in bone marrow cells of Chinese hamster.The FAGP extract also in-duced 6-thioguanine-resistant (6-TG~r) mutants in V_(79) cells in the presence ofmicrosomal preparations.Both extracts-increased the recovery of 6-~rTG V_(79) cells,when wild-type...

Extracts of cornbread inoculated with fungi isolated from Linxian,Fusali-um moniliforme (FM),or Fusalium mo-niliforme,Aspergillus flavus,Geotrichum can-didum,Penicillium cyclopium (FAGP),in-creased significantly the frequency of sister chromatid exchange in V_(79) cells and in bone marrow cells of Chinese hamster.The FAGP extract also in-duced 6-thioguanine-resistant (6-TG~r) mutants in V_(79) cells in the presence ofmicrosomal preparations.Both extracts-increased the recovery of 6-~rTG V_(79) cells,when wild-type V_(79) cells (6-TG sensitive) and 6-TG~r V_(79) cells were cultured together.The results indica-te the possible presence of mutagen(s),and promoter (s) in the moldy foods,the consumption of which might play an important role in the etiology of cancer of the esophagus in Linxian.

把从林县分离出的串珠镰刀菌(FM),或串珠镰刀菌,黄曲霉,白地霉,圆弧青霉(FAGP)分别接种于玉米面窝头。这两种霉变食物的提取物明显增加V_(79)细胞和中国地鼠骨髓细胞的姐妹染色单体交换。接种FAGP的窝头提取物,在加入肝微粒体酶制备物后,还能诱导V_(79)细胞6-琉基鸟嘌呤耐受突变种(6-TG耐受)。将V_(79)野生型细胞(6-TG敏感)和V_(79)突变型细胞(6-TG耐受)混合培养时,这两种提取物都可以增加6-TG耐受V_(79)细胞的集落形成率。初步实验结果指出,霉变窝头中可能包含致突变物和促癌物。食用霉变食物对林县食管癌的发生可能起重要作用。

The virus severely affecting papaya(Carica papaya)plantation in South China had a narrow host range ithin ome species of Caricaceaeand Cucurbitaceae.It had a dilution end-point of 10~(-2) to 10~(-3),a thermalinactivation emperature between 50 and 55℃ and a longevity in vitro bet-ween 8 and 16 hours The particles of the virus were lexuous rods bet-ween 600 and 800 nm in length and between 10 and 15 nm in diameter.There were typical pinwheel nclusion bodies in the cytoplasm of the in-fected host tissues characteristic...

The virus severely affecting papaya(Carica papaya)plantation in South China had a narrow host range ithin ome species of Caricaceaeand Cucurbitaceae.It had a dilution end-point of 10~(-2) to 10~(-3),a thermalinactivation emperature between 50 and 55℃ and a longevity in vitro bet-ween 8 and 16 hours The particles of the virus were lexuous rods bet-ween 600 and 800 nm in length and between 10 and 15 nm in diameter.There were typical pinwheel nclusion bodies in the cytoplasm of the in-fected host tissues characteristic of the potato virus Y group.The virusresponsible for the papaya disease was identified as a Chinese strain ofpapaya ringspot virus.Due to the very ow concentration of the virus in host tissues,andthe tendency to aggregate and become inactivated,many purification ro-cedures have failed.After the diseased leaves were homogenized withphosphate buffer,clarified progressively ith thyl ether and three cyc-les of differential centrifugation,the partially purified preparation of thevirus was obtained.

为害华南番木瓜的病毒以番木瓜科(Caricaceae)和葫芦科(Cucurlitaceae)中若干植物为寄主,范围狭窄。病毒的稀释终点为10~(-2)~10~(-3);热灭活点为50~55℃;体外存活期为8~16小时。病毒颗粒长约600~800nm、宽约10~15nm的略为弯曲的细丝。在感病番木瓜组织的细胞内,电镜观察到典型的风轮状内含体(pinwheel inclusion body)。病毒具有马铃薯Y病毒组成员的典型特征,可认为是国外报道的番木瓜环斑病毒的中国株系,初步命名为华南番木瓜环斑病毒。由于此病毒极易聚集和失活,多种提纯方法归于失败。用磷酸缓冲液将感病番木瓜叶匀浆、乙醚逐次澄清和三次差迷离心循环,可得到部分纯化的病毒制备物

 
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