A ΔnifZ Av1 preparation with high purity of >90% was obtained by the more fine collection on the Sephacryl S-200 and NaCl gradient elution on the Q-Sepharose.
Through the anaerobic chromatography on the columns of DEAE 52, Q-Sepharose and Sephacryl S-200, the purer ΔnifZ MoFe protein (ΔnifZ Av1) preparation was obtained from the crude extract of a nifZ-deletion mutant of Azotobacter vinelandii (DJ194).
The Km of the enzyme was 6.67 mM when xylose was used as the substrate. The enzyme was activated by Mg2+, Co2+ and Mn2+ and inhibited by Zn2+, Cu2+ and Fe3+.
Hybridomas are prepared by the fusion of mouse myeloma cells,cell line P_3-X63-Ag8.653,and spleen cells from BALB/C mice immunized with partially clarified and concentration sap from Citrus tristeza virus(CTV) infected citron.
Conformal Coating, -Part Addition-Curing These figures are intended as a guide and should not be used in preparing product specifications.
例句来源
These figures are intended as a guide and should not be used in preparing product specifications.
例句来源
In a previous report it was shown that the activity of coupling factors isolated from chloroplasts of other plant species was higher than those obtained from chloro- plasts of the same species for restoring the cyclic photophosphorylation activity of the deficient particles.However,the coupling factors then used were crude extracts or extracts after partial purification only.Verification of the results with purified preparation was highly desirable. To this end several methods of extraction and purification...
Extracts of cornbread inoculated with fungi isolated from Linxian,Fusali-um moniliforme (FM),or Fusalium mo-niliforme,Aspergillus flavus,Geotrichum can-didum,Penicillium cyclopium (FAGP),in-creased significantly the frequency of sister chromatid exchange in V_(79) cells and in bone marrow cells of Chinese hamster.The FAGP extract also in-duced 6-thioguanine-resistant (6-TG~r) mutants in V_(79) cells in the presence ofmicrosomal preparations.Both extracts-increased the recovery of 6-~rTG V_(79) cells,when wi...
The virus severely affecting papaya(Carica papaya)plantation in South China had a narrow host range ithin ome species of Caricaceaeand Cucurbitaceae.It had a dilution end-point of 10~(-2) to 10~(-3),a thermalinactivation emperature between 50 and 55℃ and a longevity in vitro bet-ween 8 and 16 hours The particles of the virus were lexuous rods bet-ween 600 and 800 nm in length and between 10 and 15 nm in diameter.There were typical pinwheel nclusion bodies in the cytoplasm of the in-fected host tissues chara...