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制备疫苗
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  generate vaccines
     As recombinant M protein and S protein can be used as antigens to detect coronavirus infection and to generate vaccines, the expression of these two genes is definitely necessary.
     重组M蛋白和S蛋白可被用来作为抗原检测冠状病毒的感染和制备疫苗
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  “制备疫苗”译为未确定词的双语例句
     Two high titered anti-idiotypic antibodies secreted by the 2H1D1 showed excellent neutralization reaction upon AIV on MDCK cells, and the PD50 were 10-1.8/10 μl and 10-1.6/10 μl respectively.
     用2H1D1分泌的抗独特型单克隆抗体制备疫苗与抗AIV灭活疫苗同时免疫SPF鸡收获的高免血清与AIV在MDCK细胞上进行中和实验,中和效价分别为10-1.8/10μl、10-1.6/10μl。
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     The C and D vaccine were the H22 cells were modified by TNF\|αgenes or were treated by means of freezing or not.
     另外 ,用未经TNF α基因修饰行冷冻处理和未行冷冻处理的H 2 2细胞 ,分别制备疫苗C和D。
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     High titer serum and immunoglobulin G (IgG) were prepared from specific pathogen free (SPF) chickens immunized with inactivated AIV subtype H9N2. Rabbits were immunized with this chicken anti-H9N2-AIV IgG and high titer serum were collected.
     将H9N2亚型AIV增殖,经差速离心和蔗糖密度梯度离心提纯后,灭活,免疫SPF鸡,制备高免血清并提纯免疫球蛋白G(IgG)。 以此制备疫苗免疫家兔,制备高免血清并提纯其中的IgG。
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     In order to prove the adjuvant effect of CpG DNA recombinant plasmid,the total antibodies and their IgG2a subtype induced by antigen of Cysticercus cellulosae,and content of IL-4 and IFN-γ secreted from splenic cell of mouse immunized were measured.
     用CpG基序寡核苷酸的重组质粒(CpGDNA)与猪囊尾蚴抗原制备疫苗免疫小鼠,检测小鼠抗囊尾蚴总抗体和IgG2a抗体亚类以及体外诱导免疫脾细胞分泌白介素-4(IL-4)和干扰素-γ(IFN-γ)的含量,观察其免疫佐剂效应。
短句来源
     Methods: H22 cells which had been modified by TNF-α genes were treated by means of freezing and 60Co radiation or of 60Co radiation, respectively. Then, they were put to use to produce two kinds of vaccines.
     方法:分别用冷冻和60Co照射处理或者单独用60co照射处理TNF-α基因修饰的H22细胞,分别制备疫苗
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  相似匹配句对
     Both vaccines developed are safe and stable.
     制备疫苗是安全、稳定的;
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     A Vaccine to Coxsackievirus Prepared by High Pressure
     高压力制备柯萨奇病毒疫苗
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     Preparation of furfuryl amine
     糠胺的制备
短句来源
     The Preparation of Copper Carbonate Hydroxide
     碱式碳酸铜的制备
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     Study on Continuous Multi-Harvesting Process for Measles Vaccine Production
     癌症疫苗
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  generate vaccines
Provoking the body's immune system to generate vaccines against cancer is emerging as an important type of immunotherapy to treat cancers.
      
A fast and reliable mechanism to discern and generate vaccines for such attacks is vital for the successful protection of networks and systems.
      
The first and more immediately accessible uses existing technology to generate vaccines that induce highly functional and protective Abs.
      


A new attenuated Type Ⅰ poliovirus (Zhong Ⅰ_9 strain) was obtained by treating the original seed strain, isolated from a poliomyelitis patient, with U.V.radiation, passing it in rhesus monkey kidney cells for 10 passages and purifying it 5 times with plaque technic. The gene- tic markers of Zhong Ⅰ_9 strain in vitro (T. d and S properties) were studied and showed negative results. No clinical or pathological change of poliomyelitis was found in 61 rhesus monkeys after intracerebral inoculation of Zhong Ⅰ_9 strain....

A new attenuated Type Ⅰ poliovirus (Zhong Ⅰ_9 strain) was obtained by treating the original seed strain, isolated from a poliomyelitis patient, with U.V.radiation, passing it in rhesus monkey kidney cells for 10 passages and purifying it 5 times with plaque technic. The gene- tic markers of Zhong Ⅰ_9 strain in vitro (T. d and S properties) were studied and showed negative results. No clinical or pathological change of poliomyelitis was found in 61 rhesus monkeys after intracerebral inoculation of Zhong Ⅰ_9 strain. Only a very slight neuro-virulence was present in 32 monkeys after intraspinal injection. The genetic markers remaingd unchanged after passing it 8~10 times at 34 or 37℃. The intratypic antigenic characters of Zhong Ⅰ_9 were different from those of Sabin LSc, tab strain. Authors suggest that Zhong Ⅰ_9 strainmay be used as seed strain for live poliovirus vaccine production.

从我国流行的Ⅰ型脊髓灰质炎野毒株中选育出一株减毒变异株——中19株。该毒株体外遗传特征均属阴性;对恒河猴嗜神经毒力低;试管连续传代后遗传学性质稳定;型内抗原性与Sabin LSc,2ab株呈异源关系。该毒株制备的疫苗完全符合我国有关活疫苗检定规程的要求,作者认为可为生产Ⅰ型脊健灰质炎活疫苗提供新的毒种。

Four types of cell culture,namely,HEP-2,HeLa,human embryo lung fibroblast and chicken embryo fibroblast were infected with 4 st- rains of herpes simplex virus,i.e.CC21,F,W,and G,the formal 2 of type 1 and W and G of type 2.The SDS-PAGE maps showed dif- ference between the maps form at 4h after infection and that at twenty four hours after virus infection,the electrophoresis maps showed a dif- ference between infected and uninfected cells.In the chicken embryo fi- broblast culture,there were difference between...

Four types of cell culture,namely,HEP-2,HeLa,human embryo lung fibroblast and chicken embryo fibroblast were infected with 4 st- rains of herpes simplex virus,i.e.CC21,F,W,and G,the formal 2 of type 1 and W and G of type 2.The SDS-PAGE maps showed dif- ference between the maps form at 4h after infection and that at twenty four hours after virus infection,the electrophoresis maps showed a dif- ference between infected and uninfected cells.In the chicken embryo fi- broblast culture,there were difference between maps of HSV-1 and that of HSV-2.This was to mean that by the chicken embryo fibroblast sys- tern HSV-1 could be differentiated from HSV-2 by the SDS-PAGE meth- od.Therefore the PAGE technique might be used asamethod to classify the types of herpes simplex virus. After sucrose gradient density centrifugation the SDS-PAGE techni- que showed 12 bands in HSV-1 and 15 bands in HSV-2 respectively.A discussion was made on some problems of selection of types of cell cul- ture in case the vaccine production is considered.

我们用 SDS—PAGE 测定四株 HSV 感染 HEp—2细胞、HeLa 细胞、人胚肺细胞、鸡胚细胞多肽与未感染细胞多肽的电泳图结果表明:四小时感染细胞多肽与未感染细胞多肽相比未见有区别,二十四小时的感染细胞多肽与未感染细胞多肽的电泳带显示有所区别。其中感染 HSV—1和 HSV—2型的鸡胚细胞多肽电泳带亦显示有所区别。说明鸡胚细胞对 HSV—1型和 HSV—2型的敏感性差异,亦可以从电泳带反映出来,说明电泳带可作为 HSV 分型的又一方法。经蔗糖梯度超离心纯化的未标记同位素的地方株 HSV—1CC21株和 HSV—2W 株病毒颗粒用 SDS 裂解,并进行 SDS—PAGE 表明这两株病毒的结构多肽分别为12和15种,显示型的区别。本文还就关于在制备疫苗时以选择何种细胞为宜的问题进行了讨论。

We observed and analysed the expression characteristics of HBsAg in the transformed cell line MT-5. The results revealed that the expression and excretion of HBsAg is stable; the cells could stand 200μm ZnCl, and the yield of HBsAg could be induced by ZnCl2 up to 2.5mg/l, we established several subcloned cell lines of MT-5 and The expression yield of HBsAg is different in different sudline HBsAg expressed by MT-5 showed 23K and 27K polypeptides by SDS-PAGE analysis. It is evident that the HBsAg can be glycosylated...

We observed and analysed the expression characteristics of HBsAg in the transformed cell line MT-5. The results revealed that the expression and excretion of HBsAg is stable; the cells could stand 200μm ZnCl, and the yield of HBsAg could be induced by ZnCl2 up to 2.5mg/l, we established several subcloned cell lines of MT-5 and The expression yield of HBsAg is different in different sudline HBsAg expressed by MT-5 showed 23K and 27K polypeptides by SDS-PAGE analysis. It is evident that the HBsAg can be glycosylated in MT-5 cells.

作者对基因工程转化细胞株MT-5的HBsAg表达特性进行了观察分析。结果表明:细胞的连续传代对HBsAg的表达稳定性没有影响;MT-5的不同单细胞亚克隆株中的HBsAg表达量存在不均一性;HBsAg的表达不但受重金属离子的诱导,而且细胞可以耐受较高浓度ZnCl_2的处理;MT-5细胞表达的HBsAg具有糖基化多肽,是制备疫苗的理想材料;通过培养条件最优化,筛选高产亚克隆细胞株和采用最适重金属离子诱导浓度,HBsAg的产量达到2.5mg/1,即2.2×10~(-12)g/细胞。

 
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