The result shows that the full-length cDNA of Mn-Sod consists of 958bp including an ORF encoding for 224 amino acids and a terminate codon, while the length of genomic sequence is 1416bp including 4 exons and 3 introns, which is different from the gene of human (5 exons and 4 introns) and that of higher plant (6 exons and 5 introns).
The results were as follows: 1、The sequence of amplified horse GH gene was 1923bp, including five exons and four introns. The length of five exons were 10bp, 161bp, 117bp, 162bp and 201bp.
Compared with cDNA sequence revealed that there were five exons and four introns and the boundary of exon and intron abided by the role of GT…AG strictly.
The OPG/OCIF gene is a single copy gene consisting of five exons and four introns. It is located in 8q23~24. The factors involved in the regulation of bone formation and resoption(eg. TGF β1,1,25(OH) 2VD 3 and TNF α)regulate the expression of OPG/OCIF gene.
In this paper, we report the sequences of two ST8Sia I mRNAs found in Xenopus laevis and their genomic organization; the canonical form resulted constituted of 5 exons and 4 introns, while the "short" mRNA lacks of the exon 2.