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      低温保存时
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  “低温保存时”译为未确定词的双语例句
     Additionally, the viability, survival time and survival index of spermatozoa from nine male blue foxes were compared in low and normal temperature storage in this study.
     低温保存时最长存活时间和生存指数分别为:108小时、48.4,平均存活时间和生存指数为:37小时、17.89。 蓝狐精液在常温保存时的存活时间、生存指数与低温保存时的存活时间和生存指数之间具有线性关系。
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     Human liver sinusoidal lining cells damage following cold storage
     肝脏低温保存时肝窦内皮细胞损伤的研究
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     But BSA couldn't significantly improve the stability of the enzyme in crude preparations when stored at higher temperature.
     一定浓度的甘油可提高乙酰胆碱酯酶在低温保存时的稳定性,但加入稳定剂牛血清白蛋白,对粗酶液中酶的稳定性并不能产生显著效果。
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     5、The application of the platform for organ preservation and the preservation apparatus for organ indicated that① Cells such as 293 and PK15 cell lines could be successfully cultred in this machine. When temperature was going down as designed,the morphological changes of cells could be found;
     5、待移植器官体外保存试验平台和待移植器官体外保存系统原理机初步应用表明:① 293细胞、PK15细胞在低温保存时细胞形态发生较大变化,表现为细胞皱缩、突起变长等特征,在该条件下,这些细胞停止增殖;
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     Histological Analysis Grade of PHC-A solution was superior to HC-A solution (p<0. 01) and UW solution (p<0. 05)Conclusion:amending HC-A solution containing PEG 20000 present better protection to the hypothermal preservation of the isolated perfused rat kidney ?
     组织病理学改变的评分结果显示PHC-A液组明显优于HC-A组(p<0.01),与UW液差别明显,(p<0.05)。 结论:加入PEG的改良HC-A液对高体肾脏进行低温保存时,在防止间质水肿,脂质过氧化以及保持组织结构方面有良好的保护作用。
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     Study on Air Bubbles Formation in Cryopreservation Procedure
     低温保存的气泡形成现象试验研究
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     Cherishing Time
     惜
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     time and fate;
     、数;
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     Human liver sinusoidal lining cells damage following cold storage
     肝脏低温保存肝窦内皮细胞损伤的研究
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     VITRIFICATION IN LOW TEMPERATURE PRESERVATION OF CELLS, TISSUES AND ORGANS
     生物材料的玻璃化低温保存
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  cryopreservation procedure
Biological chip technology to quickly batch select optimum cryopreservation procedure
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Results showed that the viability of attached OB cells decreased significantly compared with the viability of isolated OB cells under the same cryopreservation procedure.
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Various cryoprotectants were investigated to develop a cryopreservation procedure for the infectious pre-parasitic juveniles of R.
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Pre-parasitic juveniles treated with ethanediol, hydroxyethyl starch, and polyvinylpyrrolidone as cryoprotectants did not survive the cryopreservation procedure.
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A cryopreservation procedure using encapsulation/dehydration was established for shoot-tips obtained from in vitro-grown shoots of hop.
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         We have reported cytokinin (CTK)-bind-ing proteins in chloroplasts of wheat leaves in our previous work.We found in the pre-sent work that the CTK-binding protein has a high affinity for 6-benzyladenine(6 BA).The Kd value is 3.7×10~(-3)M and the Bmax.is 10.7 pmols 6 BA per mg of protein.Scatchard plot shows only one class of binding site in the protein.The binding activity of chloroplast CTK-binding protein changes with different kinds of purification precedures.With differen-tial centrifugation,a method fo...
            叶绿体中存在着与细胞分裂素(CTK)专一结合的蛋白质。这一蛋白与6-苄氨基嘌呤(6 BA)的亲和力很强,解离常数达3.7×10~(-8)mol/L。最大结合量为10.7 pmol 6 BA/mg蛋白,Seatchard分析表明只有一类结合位点。不同的叶绿体纯化步骤对CTK结合蛋白的活性有不同的影响,分离步骤少而快速的差速离心法可以得到具有较高结合活性的叶绿体。叶绿体经分离纯化后,低温保存时的结合活性较稳定,-20℃以下可以较长期保存。用EDTA预处理叶绿体,不降低CTK结合蛋白对6 BA的结合活性,而用高浓度的NaCl处理,可以使叶绿体结合6BA的能力明显下降。这说明EDTA不能使CTK结合蛋白从叶绿体膜系统表面解离,而高浓度NaCl则有这种可能性。
文摘来源
         When the boar semen was stored at room temperature for 72 hours,the diluent containing gentamicin was superior to that containing penicillin and streptomycin for preservation. There were most significant differences in the motillty ,acrosome integrality and survival index of the sperms and GOT and LDH activities of the semen whey between these two semen samples preserved with different ways. The diluent containing honey and egg yolk was more ideal than diluent containing glucose and milk powder for preventi...
            猪精液在室温保存时,含庆大霉素的稀释液保存效果优于含青、链霉素的稀释液,两种稀释液在保存72h时,精子活率、顶体完整率、GOT和LDH活性以及存活指数差异极显著(P<0.01).低温保存时,含蜂蜜和卵黄的稀释液对防精子冷刺激、维持精子活率的效果比含葡萄糖、奶粉的稀释液理想.在冷冻保存条件下,以含2%甘油的稀释液保存效果最佳.液态保存(室温、低温)的猪精液,LDH活性随精子活率下降而下降;pH值则都呈先上升后下降的变化趋势.室温保存主要是引起精子顶体脊膨大和保存后期的顶体前端膨大;低温保存主要引起顶体前部膨大和保存后期的少数顶体脱落;冷冻保存主要是造成顶体的膨大程度加大和顶体脱落.3种保存条件都造成精子线粒体透性增强.液态保存和冷冻保存精液的精清GOT活性与顶体完整率无显著相关性(P>0.05).
文摘来源
         Vitrification has become an approach to cryopreservation only in recent years.What is vitrification, how to vitrify a liquid and the difficulty in vitrification as a method ofcryopreservation of biological systems, are discussed briefly. The application of cryopreservationby vitrification is also mentioned in this review.
            用玻璃化方法长期低温保存生物材料的研究取得了很大的进展。本文简述了玻璃化方法的基本原理、实现玻璃化的条件和用于低温保存时面临的困难和对策以及有关应用研究的进展。
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