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fsh组
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  fsh treatment
     Among the living rabbits after 3 consecutive times surgery,the efficiency of recovery in FSH treatment group and control protocols did not differ(P>0.05). However,at the FSH-PVP treatment group,themean numbers in the third surgery was significantly lower than the first(P<0.01).
     3次连续手术均成活的家兔中,对照组和FSH组3次取卵效果无显著差异(P>0.05),FSH混合25%PVP组首次手术与第三次手术卵母细胞数出现显著差异(P<0.01)。
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  相似匹配句对
     (3) Y group, only one single element Y.
     (3) Y:Y。
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     3. Experimental group(N);
     3.N;
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     There was no correlation between serum levelsof FSH and concentrations of S(?)
     CRF FSH 和BUN、S(?)
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     w3c.
     w3c .
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     3 ) .
     3).
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  fsh treatment
Treatment with both IGF-1 and FSH further shifted the grain count distribution per cell to favor granulosa cells expressing more P450scc mRNA compared to IGF-1 or FSH treatment alone (p>amp;lt;0.05).
      
Since the target tissue of FSH is the seminiferous epithelium, the observed effects on Leydig cells by FSH treatment suggest that the secretion of factors by the seminiferous tubules may mediate the maturation of Leydig cells.
      
The increase in catalase after FSH treatment was also accompanied by a similar increase in estradiol secretion.
      
Effect of Parity, Season and FSH Treatment on the Calving Interval of Ayrshire Cows in the Tropics
      
These Sertoli cell lines expressed FSH receptors and the total phosphoprotein patterns were converted after FSH treatment.
      
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Immature Sprague-Dawley rats (21-26 days old) were divided into an experimental group and a control group. The rats of the experimental group were subdivided according to the number of days of injection into 2, 3, 4, and 5 day groups. Each rat of the experimental group received an injection of DES 0.5 mg in sesame oil per day. Those of the control group received only the sesame oil. The animals were sacrificed 2-5 days after the injection. A light and electron microscopic study of the ovaries and granulosa cells...

Immature Sprague-Dawley rats (21-26 days old) were divided into an experimental group and a control group. The rats of the experimental group were subdivided according to the number of days of injection into 2, 3, 4, and 5 day groups. Each rat of the experimental group received an injection of DES 0.5 mg in sesame oil per day. Those of the control group received only the sesame oil. The animals were sacrificed 2-5 days after the injection. A light and electron microscopic study of the ovaries and granulosa cells were carried out. The granulosa cells from each experimental group were cultured in serum-free McCoy's 5a medium by adding various hormones. The cultures were carried out in the following groups: control, FSH, DES+FSH, FSH+hCG. Three days after the culture, the morphology of cells of various groups were observed by LM, TEM and SEM. Results obtained were as follows:

取出生后21~26天Sprague-Dawley未成熟雌性大鼠,分为实验与对照两组。实验组只/天注射己烯雌酚(DES)0.5mg(溶于芝麻油中),并按注射天数分为2、3、4、5天组;对照组注射等量的芝麻油。注射2天后,逐日取材固定,用光镜和电镜观察两组动物的卵巢和卵泡颗粒细胞。同时从各组卵巢中分离出卵泡颗粒细胞,用无血清McCoy 5a培养基培养,加入不同的激素,分为对照组、FSH组、DES+FSH组、FSH+hCG组。培养3天后,取材固定,用光镜、透射电镜、扫描电镜观察各组的细胞形态,结果为:1.实验组卵巢比对照组的大,卵泡也大。卵泡细胞增多,细胞体积大,分裂象多见。2.注射3天以上DES的卵巢中,闭锁卵泡和退化的卵泡细胞增多。3.电镜下,实验组卵泡细胞中与蛋白质合成有关的细胞器比对照组发达。4.体外培养的卵泡颗粒细胞,对照组细胞排列分散;FSH组细胞集聚成团,细胞表面微绒毛增多;细胞质内与合成蛋白质有关的细胞器比对照组的发达。DES+FSH组的细胞排列和结构与FSH组的相似。FSH+hCG组细胞体积增大,细胞表面微绒毛减少或消失。细胞质内出现滑面...

取出生后21~26天Sprague-Dawley未成熟雌性大鼠,分为实验与对照两组。实验组只/天注射己烯雌酚(DES)0.5mg(溶于芝麻油中),并按注射天数分为2、3、4、5天组;对照组注射等量的芝麻油。注射2天后,逐日取材固定,用光镜和电镜观察两组动物的卵巢和卵泡颗粒细胞。同时从各组卵巢中分离出卵泡颗粒细胞,用无血清McCoy 5a培养基培养,加入不同的激素,分为对照组、FSH组、DES+FSH组、FSH+hCG组。培养3天后,取材固定,用光镜、透射电镜、扫描电镜观察各组的细胞形态,结果为:1.实验组卵巢比对照组的大,卵泡也大。卵泡细胞增多,细胞体积大,分裂象多见。2.注射3天以上DES的卵巢中,闭锁卵泡和退化的卵泡细胞增多。3.电镜下,实验组卵泡细胞中与蛋白质合成有关的细胞器比对照组发达。4.体外培养的卵泡颗粒细胞,对照组细胞排列分散;FSH组细胞集聚成团,细胞表面微绒毛增多;细胞质内与合成蛋白质有关的细胞器比对照组的发达。DES+FSH组的细胞排列和结构与FSH组的相似。FSH+hCG组细胞体积增大,细胞表面微绒毛减少或消失。细胞质内出现滑面内质网,细胞开始黄体化。5.注射2~5天DES的各组细胞形态和结构均相似。从形态学来看,经DES处理的未成熟雌鼠的卵泡颗粒细胞的形态、排列和对不同激素作用下的变化,与文献报道的去垂体雌鼠的同类细胞相似。因此我们认为,未成熟雌鼠的卵泡颗粒细胞也能作为研究细胞分化的一种模型。

28 does, exhibiting normal estrus cycles, were divided into three groups to compare the ovarian response. Trial 1 were injected i.m. with 1500 iu PMSG. Trial 2 with 300 iu. FSH. Trial 3 with saline. The plasma progesterone (P_4) and oestradiol-17β(E_2-17β) concentrations were estimated at different time before and after superovulation to study their changing way. The results indicated that: (1) the ovulation rate (O.R.) for 3 groups was 12.00±1.71, 21.22±2.25 and 2.75±0.31 respectively. The effect of FSH treatment...

28 does, exhibiting normal estrus cycles, were divided into three groups to compare the ovarian response. Trial 1 were injected i.m. with 1500 iu PMSG. Trial 2 with 300 iu. FSH. Trial 3 with saline. The plasma progesterone (P_4) and oestradiol-17β(E_2-17β) concentrations were estimated at different time before and after superovulation to study their changing way. The results indicated that: (1) the ovulation rate (O.R.) for 3 groups was 12.00±1.71, 21.22±2.25 and 2.75±0.31 respectively. The effect of FSH treatment was better than that of PMSG in other respects also, e.g. the number of recovered embryos. (2) the P_4 and E_2-17β levels rose significantly after 48h of PMSG treatment, but only 24h after FSH injection. P_4 levels fell rapidly after PGF_(2α) injection (p<0.01) and reached the lowest at estrus. All goats had the highest levels of E_2-17β at this time. (3) there was no significant relationship between the P_4 levels and O.R. in 3 groups (p>0.05), but the top values of E_2-17β at oestrus were postively correlated with the O. R. (r=0.72, p<0.001).

对28只性周期正常山羊分别注射1500iu PMSG(Ⅰ组)、300iu FSH(Ⅱ组)和等量生理盐水(Ⅲ组),比较两种促性腺激素的超排效果;用放射免疫方法测定血浆孕酮(P_4)和17β-雌二醇(E_2-17β)浓度。试验发现:(1)Ⅰ、Ⅱ、Ⅲ组的排卵率依次为12.00+1.71、21.22+2.25和2.75±0.31,超排效果FSH优于PMSG。(2)PMSG和FSH处理后,P_4和E_2-17β浓度都上升,但PMSG组只有在48h后两浓度达显著水平,FSH处理只经24h就显著升高。注射PGF_(2α)后两组的P_4浓度急剧下降(ρ<0.01)。发情时P_4浓度最低,E_2-17β为最大。(3)处理当日及此后的孕酮浓度与排卵率之间无显著相关(ρ>0.05)。而发情时的E2-17β浓度与排卵率之间呈强相关(r=0.72,ρ<0.001)。

The effects of FSH (made in China or Sigma), PMSG and HCG on superovulation of 136 female rabbits during 1991 ̄1993 are reported. Results indicate that the high active FSH (made in China) has a very good superovulation effect on rabbits. Among the treated animals, 85 % responded to the treatment and an average of 31. 1 ova per rabbit were collected, which was nearly 4 times than that of the natural oestrous rabbits (8. 5).The superovulation response of rabbits is better in Spring than in Autumn. The average number...

The effects of FSH (made in China or Sigma), PMSG and HCG on superovulation of 136 female rabbits during 1991 ̄1993 are reported. Results indicate that the high active FSH (made in China) has a very good superovulation effect on rabbits. Among the treated animals, 85 % responded to the treatment and an average of 31. 1 ova per rabbit were collected, which was nearly 4 times than that of the natural oestrous rabbits (8. 5).The superovulation response of rabbits is better in Spring than in Autumn. The average number of ova collected from FSH-treated rabbits in Spring (35. 0) was significantly greater than that in Autumn (11. 9, P<0. 001), and so was the case in PMSG-administrated rabbits. In addition, the ova collection time had a clear effect on the number of ova harvested. The number of ova collected more than 13. 5 h after HCG injection was nearly double of ova collected with in 13. 5 h (36. 0 vs 18. 3). Significantly more ova were ovulated from the right ovary than from the left one during both natural oestrous and superovulation.

在1991~1993年间,应用国产和进口的促卵泡素(FSH)及国产的孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG),对136只母兔进行超数排卵处理试验。结果显示,国产的高活性FSH对母兔有良好的超排作用。试验母兔中有85%表现超排效应,平均每只母兔可回收到31.1个卵,几乎相当于对照组(自然发情组,8.5个/只)的4倍。母兔对超排的反应表现出明显的季节性,春季时的处理效果极显著地优于秋季。其中FSH组在春、秋季的回收卵数分别为35.0个/只与11.9个/只,差异十分显著(P<0.001);PMSG组也表现类似效果。另外,注射HCG后不同回收时间对卵回收数的影响也很大。在注射了HCG13.5h后卵母细胞的回收数非常显著地比13.5h前高,两者几乎相差1倍(36.0:18.3)。同时,母兔左、右侧卵巢排卵数也表现出明显差异,不论是自然发情或人工超排,母兔右侧卵巢的排卵数均显著地高于左侧卵巢。

 
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