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  icr mice
Twenty-five tiptoe-walking Yoshimura (twy) mice with calcified mass lesions compressing the spinal cord posterolaterally at the C1-C2 vertebral levels were compared with five Institute of Cancer Research (ICR) mice that served as controls.
      
albicans) in mice, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C.
      
To study the effect of itraconazole on the vaginal candidiasis caused by Candida under different immunity conditions, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C.
      
The relationship between Ni-induced hepatic lipid peroxidation (LPO) and the concentrations of Ni and trace elements was investigated in male ICR mice.
      
The l-SeMet α,γ-elimination enzyme was ubiquitous in tissues from ICR mice and the activity was relatively high in the large intestine, brain, and muscle, as well as the liver.
      
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Conditioned media were prepared by using the collected media which had cultured fibroblasts from muscle tissue of fetal mouse (ICR) or chick embryo (Leghorn chicken). The effects of these media on the proliferation and fusion in mouse o(?) chick myoblast were studied quantitatively.The results were as follows: (1) the conditioned medium from fibroblasts of fetal mouse promoted the proliferation of mouse or chick embryonic myoblasts by 2.65 times (P<0.001) or 2.35 times (P<0.01) as compared with control group(?)...

Conditioned media were prepared by using the collected media which had cultured fibroblasts from muscle tissue of fetal mouse (ICR) or chick embryo (Leghorn chicken). The effects of these media on the proliferation and fusion in mouse o(?) chick myoblast were studied quantitatively.The results were as follows: (1) the conditioned medium from fibroblasts of fetal mouse promoted the proliferation of mouse or chick embryonic myoblasts by 2.65 times (P<0.001) or 2.35 times (P<0.01) as compared with control group(?) respectively. (2) The conditioned medium from fibroblasts of embryonic chicken promoted the proliferation of chick or mouse embryonic myoblasts by 2.66 times (P<0.01) or 2.17 times (P<0.01) respectively. (3) The conditioned medium from fibroblasts of fetal mouse enhanced the fused rate of mouse or chick myoblasts by 1.9 or 2.6 times respectively. The conditioned medium from fibroblasts of embryonic chick enhanced the rate of chick myoblast fusion by 2.1 times, but the effect on the mouse myoblast fusion was not remarkable.The results suggest that the effect of fibroblast conditioned media on myoblast proliferation is similar in the two species, but the effect on the fusion of myoblasts is somewhat species-specific.

用培养过鸡胚(来亨鸡)或胎鼠(ICR小鼠)肌组织的成纤维细胞的条件培养液,定量地研究它们对胎鼠或鸡胚的成肌细胞的增殖和融合的影响。所得结果如下:(1) 胎鼠的成纤维细胞条件培养液促进胎鼠或鸡胚成肌细胞增殖,分别为对照组的2.65倍,(P<0.001)或2.35倍,(P<0.01);(2) 鸡胚的成纤维细胞条件培养液促进鸡胚或胎鼠的成肌细胞增殖,分别为对照组的2.66倍,(P<0.01)或2.17倍,(P<0.01);(3) 胎鼠的成纤维细胞条件培养液增加胎鼠或鸡胚的成肌细胞的融合率,分别为对照组的1.9倍或2.6倍;鸡胚的成纤维细胞条件培养液只增加鸡胚成肌细胞的融合率,为对照组的2.1倍,但对胎鼠成肌细胞的融合无明显的影响。 实验结果提示:成纤维细胞条件培养液促进成肌细胞的增殖,两种动物间无明显的差异,但在融合上却有一定的种属特异性。

The ICR mice were used as donors of fertilized eggs inthis study. A linear HuIFN-β foreign gene was introduced into pronuclei of fertilized eggs at a dosage of 1-3 pL, 2940-10000 copies per egg by micro-injection. 301 fertilized eggs which receivcd foreign gene were transferred to20 pseudopregnant recipients. 25 offsprings were totally obtained. DNAwas extracted from the tails of founder mice and cut with BamHI. Theintegration of foreign genes in the genomes of those mice was tested bySouthern blot analysis....

The ICR mice were used as donors of fertilized eggs inthis study. A linear HuIFN-β foreign gene was introduced into pronuclei of fertilized eggs at a dosage of 1-3 pL, 2940-10000 copies per egg by micro-injection. 301 fertilized eggs which receivcd foreign gene were transferred to20 pseudopregnant recipients. 25 offsprings were totally obtained. DNAwas extracted from the tails of founder mice and cut with BamHI. Theintegration of foreign genes in the genomes of those mice was tested bySouthern blot analysis. Some transgenic mice carrying pHI-β-IFN weredemonstrated with cut technique. DNA of mice which were borne bytransgenic mice was cut with aforecited EcoR I. It was shown that fragmentsof DNA cut with above restriction enzyme from different tissues of most ofthose mice were found to produce multiple bands with sizes of more than5.0 kb and a faint 1.8 kb band, representing intact pHI-β-IFN, pSV_2vector and HuIFN-β gene, respectively. Expression of HuIFN-β intransgenic mice was detected with CPE inhibition. It was found that thefluid of tumour tissue from No. 8-2 mouse contained biologically activeHuIFN-β with titer of 160 U/mL. This result showed that foreign genesintegrated to genome of transgenic mice can express to a certain extentwithout any induction.

选择ICR小鼠为受试动物,应用显微注射仪,将线状人β干扰素基因,按2940~10000拷贝,1~3pL的剂量注射到供体受精卵的雄前核,然后移植到假孕受体的输卵管。实验先后注射了301个受精卵,移植到20只假孕受体,其中5只妊娠,产仔25只。从所获得的25只亲代小鼠的尾组织提取DNA,用内切酶BamHI消化,以[α-~(32)P]-dCTP标记的上述外源基因为特异性核酸探针,应用DNA印迹法检测这些小鼠DNA内外源基因的整合状况.结果表明,亲代小鼠中有人β干扰素基因重组子的转基因小鼠,杂交带在6.8kb位置。应用上述同样方法对亲代转基因小鼠的子1代组织DNA检测发现,绝大多数小鼠的标本除见到1条1.8kb人β干扰素基因的较弱杂交带外,还有数条5.0kb以上强弱各异的杂交带,表明这种外源基因不仅整合到了亲代小鼠的遗传物质内,而且可经垂直方式传给子代。人β干扰素基因的鼠体水平表达检测发现,1只子1代转基因小鼠的肿瘤组织浸出液中含有人β干扰素,滴度达160U/mL,表明整合在小鼠DNA内的人β干扰素外源基因不经诱导而有一定表达。

The genetic character of two colonies of ICR outbred mice and one colony of KM outbred mice in Shanghai area was studied by authors using biochemical mar- kers and Ouchterlony methods.The phenotypes of 120 mice in 10 loci were tested, then the phenotype frequency and gene frequency in each colony were calculated. The results of comparative studies showed:(1)in eight loci,there are quite dif- ferent phenotype frequency and gene frequency between two ICR colonies,especially in Es-3,Gpd-1,Hc and Idh-1 loci:(2)KM...

The genetic character of two colonies of ICR outbred mice and one colony of KM outbred mice in Shanghai area was studied by authors using biochemical mar- kers and Ouchterlony methods.The phenotypes of 120 mice in 10 loci were tested, then the phenotype frequency and gene frequency in each colony were calculated. The results of comparative studies showed:(1)in eight loci,there are quite dif- ferent phenotype frequency and gene frequency between two ICR colonies,especially in Es-3,Gpd-1,Hc and Idh-1 loci:(2)KM colony has six kinds of phenotype in Es-3 locus and polymorphism is the strain character of KM mice.In six loci(Car-2, Es-3,Gpd-1,Hbb,Idh-1 and Mod-1),the gene frequency of KM colony approaches ICR colony Ⅱ;(3)in Es-3,Hbb,Hc,Idh-1,and Mod-1 loci,ICR colony I ap- proaches the control substrain Jct:ICR;but in Gpd-i locus,Jcl:ICR js aproached by ICR colony Ⅱ;in Car-2 locus,Jcl:ICR has its own character. The relationship between the histories of ICR,KM mice and their geneic character was discussed.Authors have indicated the importance of genetic control in reproduction of outbred mice.

对上海地区 ICR 系小鼠的2个远交群和昆明系(KM)小鼠的1个远交群共120只小鼠,用同工酶电泳法和免疫双向扩散法在10个位点上进行了基因型测试,分别计算其表型频率和基因频率,对 ICR 群体之间、ICR 与 KM 群体之间以及国内外 ICR 群体之间的遗传特性进行了比较研究。在被测3个群体和对照用1个群体中,ICR 群体Ⅰ的遗传性状同对照群相近,ICR 群体Ⅱ的遗传性状同 KM 群体相近。本文还探讨了 ICR小鼠和 KM 小鼠的历史渊源与其遗传特性的联系。

 
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