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blast序列
相关语句
  blast sequence
     ③PGEM-T/Ang-1 vector was constructed successfully, and Blast sequence analysis is in accordance with AY121504 in GenBank.
     ③成功构建了PGEM-T/Ang-1载体,Blast序列分析与GenBank中AY121504一致。
短句来源
     The first 20 amino acid sequence of CVNPF from N terminal as NLYQFKNMIQCTVPSRSWWD was obtained, and CNPF was identified as a member of secreted phospholipase A2s(sPLA2s) by BLAST sequence alignment. Inhibitors of sPLA2s (7,7 Dimethyleico sadienoic acid and Manoalide) can not block its neuronal protection.
     神经保护因子(Cobravenomneu-ronalprotectivefactor,CVNPF)此神经保护因子N-末端20个氨基酸序列为NLYQFKNMIQCTVPSRSWWD-,BLAST序列同源比对确定该保护因子为中华眼镜蛇分泌型磷脂酶A2(secretedphospholipaseA2,sPLA2)。
短句来源
     A 1.5 kb fragment of cDNA was amplified by RT-PCR,and PGEM-T/Ang-1 vector was successfully established. Blast sequence analysis was coincident with AY121504 in GenBank.
     RT-PCR扩增出1.5kb的cDNA片段,成功构建了PGEM-T/Ang-1载体,Blast序列分析与GenBank中AY121504一致。
短句来源
     Results High-quality total RNA was extracted from human placenta. A fragment of human angiopoietin-1 gene, 1.5kb, was amplified by RT-PCR. PGEM-T/Ang-1 vector was constructed successfully, and the result of Blast sequence analysis is in accordance with that of AY121504 in GenBank.
     结果从胎盘组织总RNA中,RT-PCR扩增出1.5kb的cDNA片段,成功构建了PGEM-T/Ang-1载体,B last序列分析与GenBank中AY121504一致。
短句来源
  “blast序列”译为未确定词的双语例句
     BLAST results revealed that the HA gene represented the H6 subtype and the NA gene represented the N2 subtype.
     BLAST序列比较结果显示,该毒株HA基因属于H6亚型,NA基因属于N2亚型。
短句来源
     BLAST was used to search the NCBInr-database at http://www. ncbi. nlm.
     通过NCBI网站(http:/www.ncbi.nlm.nih.gov)作Blast序列同源性分析。
短句来源
     Sequence analysis indicated that the cDNA sequence of OsFNR is identical to that of FNR from rice embryo.
     BLAST序列同源性分析表明,该基因cDNA序列与水稻胚中的FNR同源,后者仅有电子登记,但没有进一步的研究报道。
短句来源
     The result showed that the Myf-5 gene (5219bp) consisted of exon 2, partial sequence of intronl and 3. The comparison analysis of the fragment sequence with Bos taurus through blast of GenBank, the homologies of the nucleotide sequence of Sanjiang indicus、Dechang buffalo、Gayals with Bostaurus were 99.2%;
     结果表明:所测序列包括Myf-5因(5219bp)的全部外显子2序列和内含子1、3的部分序列。 通过Blast序列比较分析,三江黄牛、德昌水牛、大额牛3个牛种的Myf-5基因均在1521位点发生了A-T的转换或者颠换,与普通牛种同源性均为99.2%;
     Methods NGAL gene 5′ nontranscribed region was cloned by PCR. After sequencing the base pair mutation character was confirmed by using NCBI/BLAST database.
     方法 :采用PCR法克隆NGAL基因 5′端转录调控区 ,并通过NCBI公共数据库BLAST序列分析鉴定突变。
短句来源
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  相似匹配句对
     p sequences or R.
     p序列和R.
短句来源
     It generates 2~~(s·g(n,s)) M sequences of span.
     g(n,s)个n级M序列
短句来源
     there are 32 sequences producing significant alignments and containing above 52% identities with the predicted protein;
     BLAST分析,发现同源性52%以上的序列32 条;
短句来源
     Construction of Local Database and Achieving Local BLAST of Bt cry Genes
     Bt cry序列本地数据库的建立及本地BLAST的实现
短句来源
     Parallel Detection Algorithm of V-BLAST
     V-BLAST的并行译码
短句来源
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  blast sequence
The World Wide Web provides a vast resource to genomics researchers, with Web-based access to distributed data sources such as BLAST sequence homology search interfaces.
      
Based on the Blast sequence comparisons, an intron was predicted to be present in both genes.
      
BLAST sequence homology searches were performed in order to identify the template proteins.
      
Figure 1 shows the execution profile from gprof for our three applications and the BLAST sequence analysis application.
      
For instance, in the example query, the call to the BLAST sequence similarity algorithm.
      
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Genomic DNA and cDNA fragments encoding para sodium channel were cloned from cotton aphid, Aphis gossypii Glover using Touch down PCR technique with degenerate primer. The amplified cDNA fragment is of 659bp, which demonstrate high identity with para sodium channel gene amplified from other insects through Blast sequence analysis. Genomic DNA is of 1328bp, including 2 introns. Cloning of cDNA fragments from Aphis gossypii bear great significance on the pesticides molecular design and pest resistance research.,...

Genomic DNA and cDNA fragments encoding para sodium channel were cloned from cotton aphid, Aphis gossypii Glover using Touch down PCR technique with degenerate primer. The amplified cDNA fragment is of 659bp, which demonstrate high identity with para sodium channel gene amplified from other insects through Blast sequence analysis. Genomic DNA is of 1328bp, including 2 introns. Cloning of cDNA fragments from Aphis gossypii bear great significance on the pesticides molecular design and pest resistance research., especially pest resistance molecular monitoring.

采用降落 PCR技术 ,根据昆虫 para型钠通道 区 S4至 S6及 与 连接区的保守区域设计简并引物 ,成功地克隆了棉蚜 para型钠离子通道 c DNA和基因组 DNA片段 (Gene Bank登录号分别为 :AF4 114 5 5、AF4 475 74 ) ,其中 c DNA片段为 6 5 9bp,编码 2 2 0个氨基酸。利用 c DNA片段设计特异性引物克隆获的基因组DNA片段为 132 8bp,共有 2个内含子。Blast序列分析表明 ,PCR扩增获得的棉蚜 para型钠离子通道 c DNA片段所编码的氨基酸与其他昆虫的 para型钠通道氨基酸具有很高的同源相似性 ,与马铃薯甲虫、德国小蠊、果蝇和家蝇的同源性分别为 6 5 %、6 3%、6 3%、5 9%。棉蚜 para型钠离子通道 c DNA和基因组 DNA片段的克隆对于农药分子设计与害虫抗性机制 ,特别是靶标抗性分子监测具有重要意义。

Objective To study the method for rapid preparation of the gene chip probes for Bacillus anthracis protective antigen (pag). Methods According to the sequences of pag published on PubMed, a pair of primers was designed to amplify the PA gene of Bacillus anthracis. Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. The DNA sequences of these segments were determined with 377 DNA Sequencer, and the resultant sequences...

Objective To study the method for rapid preparation of the gene chip probes for Bacillus anthracis protective antigen (pag). Methods According to the sequences of pag published on PubMed, a pair of primers was designed to amplify the PA gene of Bacillus anthracis. Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. The DNA sequences of these segments were determined with 377 DNA Sequencer, and the resultant sequences analyzed with Bioinformatic software. Results With the designed primers, the pag 2 205 bp in length was amplified. After enzyme digestion and AT clone, 7 fragments of varied lengths were screened, which underwent analysis with the basic local alignment sequence tool (BLAST) and 377 DNA Sequencer. BLAST analysis showed that all the fragments cloned belonged to Bacillus anthracis. Conclusion PCR in conjunction with restriction enzyme digestion and AT clone is rapid and simple to prepare the desired gene chip probes.

目的快速制备炭疽杆菌保护性抗原基因芯片探针。方法根据PubMed上公布的炭疽杆菌保护性抗原的DNA序列设计引物,扩增保护性抗原基因。利用酶切、AT克隆方法快速分析筛选出保护性抗原基因片段的重组子,从而制备成芯片探针。DNA自动分析仪对克隆片段进行序列测定,生物信息学软件对其基因序列进行分析。结果根据炭疽杆菌保护性抗原设计的引物,可以扩增出长2 205 bp的保护性抗原基因,经酶切、AT克隆方法筛选出约7个长度不一的片段,对其片段进行序列测定并进行Blast序列比对得知这些片段均属于炭疽杆菌保护性抗原基因。结论利用PCR扩增产物并结合酶切、AT克隆方法可以快速、简便地制备基因芯片探针。

To isolate and identify a neuronal protective factor from Naja naja atra venom, and then explore its protective mechanism against neuronal apoptosis. The neuronal protective component was isolated and purified from Naja naja atra crude venom by continuous chromatography. The protein sequencing was carried out using Edman N terminal sequencing method, and sequence alignment was performed by BLAST (basic local sequence alignment searching tool). Neuronal protection was confirmed by the neuronal apoptosis model...

To isolate and identify a neuronal protective factor from Naja naja atra venom, and then explore its protective mechanism against neuronal apoptosis. The neuronal protective component was isolated and purified from Naja naja atra crude venom by continuous chromatography. The protein sequencing was carried out using Edman N terminal sequencing method, and sequence alignment was performed by BLAST (basic local sequence alignment searching tool). Neuronal protection was confirmed by the neuronal apoptosis model ( cerebellar granule neuronal apoptosis model induced by low potassium)A neuronal protective factor (Cobra venom neuronal protective factor,CVNPF) from Naja naja atra venom was isolated, which could protect cerebellar granule neurons from apoptosis. The first 20 amino acid sequence of CVNPF from N terminal as NLYQFKNMIQCTVPSRSWWD was obtained, and CNPF was identified as a member of secreted phospholipase A2s(sPLA2s) by BLAST sequence alignment. Inhibitors of sPLA2s (7,7 Dimethyleico sadienoic acid and Manoalide) can not block its neuronal protection.Furthermore,some other sPLA2s (from bee venom, Naja naja mossambica venom, Crotalus atroxalso venom respectively) were found to have neuronal protective function. [Conclusion]CVNPF belongs to sPLA2 family, CVNPF and some other members of sPLA2s family can protect cerebellar granule neurons from apoptosis induced by low potassium. The neuronal protection of CVNPF is independent of sPLA2s enzymatic activity.

【目的】鉴定中华眼镜蛇(Najanajaatra)蛇毒中的神经保护因子,研究其抗神经元凋亡作用机制。【方法】连续柱层析法分离、纯化蛇毒神经保护因子。用EdmanN-末端测序法取得蛋白质一级序列,BLAST软件进行蛋白质鉴定。使用低钾诱导体外培养小脑颗粒神经元凋亡模型,研究其神经元保护作用。【结果】从中华眼镜蛇蛇毒中分离出一种,可浓度依赖性地抑制低钾诱导的小脑颗粒神经元凋亡。神经保护因子(Cobravenomneu-ronalprotectivefactor,CVNPF)此神经保护因子N-末端20个氨基酸序列为NLYQFKNMIQCTVPSRSWWD-,BLAST序列同源比对确定该保护因子为中华眼镜蛇分泌型磷脂酶A2(secretedphospholipaseA2,sPLA2)。进一步发现来源于蜂毒、莫桑比克眼镜蛇(Njajanajamossambica)蛇毒、西部菱斑响尾蛇(Crotalusatroxalso)蛇毒的sPLA2也对小脑颗粒神经元具有保护作用;其作用不为磷脂酶A2酶活性抑制剂7,7-Dimethyleicosadienoicacid(DEDA)和Manoalide阻断。【结论】CVN...

【目的】鉴定中华眼镜蛇(Najanajaatra)蛇毒中的神经保护因子,研究其抗神经元凋亡作用机制。【方法】连续柱层析法分离、纯化蛇毒神经保护因子。用EdmanN-末端测序法取得蛋白质一级序列,BLAST软件进行蛋白质鉴定。使用低钾诱导体外培养小脑颗粒神经元凋亡模型,研究其神经元保护作用。【结果】从中华眼镜蛇蛇毒中分离出一种,可浓度依赖性地抑制低钾诱导的小脑颗粒神经元凋亡。神经保护因子(Cobravenomneu-ronalprotectivefactor,CVNPF)此神经保护因子N-末端20个氨基酸序列为NLYQFKNMIQCTVPSRSWWD-,BLAST序列同源比对确定该保护因子为中华眼镜蛇分泌型磷脂酶A2(secretedphospholipaseA2,sPLA2)。进一步发现来源于蜂毒、莫桑比克眼镜蛇(Njajanajamossambica)蛇毒、西部菱斑响尾蛇(Crotalusatroxalso)蛇毒的sPLA2也对小脑颗粒神经元具有保护作用;其作用不为磷脂酶A2酶活性抑制剂7,7-Dimethyleicosadienoicacid(DEDA)和Manoalide阻断。【结论】CVNPF属于sPLA2。多种sPLA2可以抗神经元凋亡,其保护机制与磷脂酶A2的酶活性无关。

 
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