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冷酚
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  cold phenol
     RNA EXTRACTION WITH COLD PHENOL METHOD AND QUALITY EXAMINATION
     冷酚法提取RNA及质量鉴定
短句来源
     Then the total atria RNA was extracted with cold phenol method and hybridized to a-32P labeled r-prepro-ANP-cDNA probe.
     用冷酚法提取心房总RNA,与α-~(32)P标记的大鼠心房肽前体原cDNA(r—prepro—ANP—cDNA)探针杂交。
短句来源
     The atrial total RNA was extracted by cold phenol method and was hybridized with a-32P labeled ANF -cDNA probe.
     用冷酚法提取心房总RNA,用α-~(32)P标记ANF—cD-NA探针,经RNA电泳转移杂交和斑点吸印杂交。
短句来源
     Methods To check tumor-loaded mice for survival rate, tumor suppressive rate, activity of NK cells, TNF-α and IFN-γ, toxicity of LTA by cold phenol extraction method and Sepharose-CL-4B purification method.
     方法冷酚法提取LTA,经SepharoseCL4B纯化,检测LTA毒性; 观察荷瘤小鼠注射LTA后的生命延长率及抑瘤率,NK细胞活性,TNFα、IFNγ分泌。
短句来源
     Atrial total RNA is extracted by cold phenol and hybridized with preproANP-cDNA probe labeled with α-32P to detect its complementary RNA that is ANP-mRNA. The result shows that the ANP-mRNA content increases after two weeks maximal training, but decreases in four or six weeks.
     冷酚法提取心房总 RNA,用 α- 32 P标记的大鼠心房肽互补 DNA探针与之杂交 ,检测ANP- m RNA的含量结果表明 ,2周大运动量训练 ,大鼠的 ANP- m RNA含量增加 ,4、6周大运动量训练大鼠的 ANP- m RNA含量减少 ;
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  “冷酚”译为未确定词的双语例句
     Large scale preparation of human IL-2 mRNA was obtained from human spleen cells stimulated with PHA+TPA at the peak of IL-2 mRNA transcription. Full length of IL-2 mRNA was translated in wheat germ extract to produce bioactive IL-2 (tIL-2).
     通过测定人IL-2和IL-2mRNA的诱生动力学,在高峰时相从PHA+TPA刺激的人脾脏单个核细胞内用冷酚法提取了大量富含IL-2mRNA的胞浆RNA。
短句来源
     The ANP-mRNA contents were determined using the Northem blot and Dot blot hybridization technique with α- 32 P-Labelled r-prepro-ANP-cDNA probe.
     冷酚法提取心房总RNA ,用 (α 32 P)标记的大鼠心钠素前体原cDNA控针与各组大鼠心房总RNA做Northernblot杂交和Dotblot杂交 ,以检测心房ANP mRNA的含量。
短句来源
     It was proved that the virus nuclear acid was DNA in the bianiline colour-show test and the ultraviolet scanning.
     用冷酚法提取的病毒核酸,经二苯胺显色及紫外扫描,证明为DNA。
短句来源
     The DNA had been extracted from the purified PoNPV particles by SDS-cold phenol method,and identified as a homogenious preparation either by Sephadex G-100 chromatography or by polyacrylamide gel electrophoresis. An approximate estimated Mol. Wt of 55 ×106 dalton was determined by restrictive enzyme digestion (EcoRI,HindⅡ) and agarose gel electrophoresis.
     经SDS—冷酚法由纯化的蜀柏毒蛾NPV颗粒抽提到了NPV的DNA.经SephadexG—100柱层析和聚丙烯酰胺凝胶电泳鉴定,该DNA是均一制剂.PoNPV-DNA的Tm值测定为70℃,经限制性内切酶HindⅢ和EcoRⅠ酶切,琼脂糖电泳分析测得该DNA分子量约为55×106道尔顿左右.
短句来源
     The method could produce cotton total RNA with higher purity and integrality in a shorter time from different tissues of cotton than other conventional methods.
     该方法与异硫氰酸胍法或冷酚法等相比具有更简便、得到的棉花组织总RNA完整性好和纯度高等优点。
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  相似匹配句对
     RNA EXTRACTION WITH COLD PHENOL METHOD AND QUALITY EXAMINATION
     法提取RNA及质量鉴定
短句来源
     On cold fusion
     核聚变
短句来源
     Cold Mountain
     《山》
短句来源
     Cold Hardened Core Box
     氨气固化芯盒和基尿烷自硬砂技术
短句来源
     Polarography of Phenol Red
     红的极谱
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  cold phenol
After 24 h RNA was extracted using the cold phenol method and separated on a tandem polyacrylamide gel.
      
Total RNAs were isolated from seedling tissues by a cold phenol method25.
      


A method to isolate and purify the messenger RNA from posterior silk gland of the Antyerea pernyi was described. The total cellular RNA was extracted by cold phenol treatment and the messenger RNA was purifed by Sepharose-2 B column. Only one band was presented in the polyacrylamiade gel electrophoritic patterns of this mRNA. In cell-free system from Enrlich ascites in the mouse with the optimal concentration of 4mM Mg~(++) and 80mM K~+ the incorporation of ~3H-Alanine stimulated by silk fibroin messenger RNA...

A method to isolate and purify the messenger RNA from posterior silk gland of the Antyerea pernyi was described. The total cellular RNA was extracted by cold phenol treatment and the messenger RNA was purifed by Sepharose-2 B column. Only one band was presented in the polyacrylamiade gel electrophoritic patterns of this mRNA. In cell-free system from Enrlich ascites in the mouse with the optimal concentration of 4mM Mg~(++) and 80mM K~+ the incorporation of ~3H-Alanine stimulated by silk fibroin messenger RNA was increased by more than 7 times.

本文介绍了两步操作提取纯化柞蚕后丝腺mRNA的方法,用SDS-冷酚法提取全核酸,通过Sepharose-2B柱层析纯化丝心蛋白mRNA,对纯化的丝心蛋白mRNA用聚丙烯酰胺凝胶电泳鉴定,达到一条电泳带的水平。此mRNA在小鼠Ehrlich腹水癌无细胞体系中,在4mM Mg~(++)和80mM K~+浓度范围内能有效地促进~3H-Ala参入达7倍以上。

Large scale preparation of human IL-2 mRNA was obtained from human spleen cells stimulated with PHA+TPA at the peak of IL-2 mRNA transcription. Full length of IL-2 mRNA was translated in wheat germ extract to produce bioactive IL-2 (tIL-2). The IL-2 mRNA was about 14 S depending on the 7 M Urea-SDS-PAGE, and recovried from gel and translated in vitro. The elute curve of tIL-2 was the same as that of recombinant IL-2 (rIL-2) and natural IL-2 (nIL-2), the molecular weight of tIL-2 was about 14.000 dalton. The...

Large scale preparation of human IL-2 mRNA was obtained from human spleen cells stimulated with PHA+TPA at the peak of IL-2 mRNA transcription. Full length of IL-2 mRNA was translated in wheat germ extract to produce bioactive IL-2 (tIL-2). The IL-2 mRNA was about 14 S depending on the 7 M Urea-SDS-PAGE, and recovried from gel and translated in vitro. The elute curve of tIL-2 was the same as that of recombinant IL-2 (rIL-2) and natural IL-2 (nIL-2), the molecular weight of tIL-2 was about 14.000 dalton. The translational process of IL-2 mRNA in vitro was inhibited when RNA was cultured with RNase or translated product was cultured with proteinase, proving a standard in-vitro translation.

通过测定人IL-2和IL-2mRNA的诱生动力学,在高峰时相从PHA+TPA刺激的人脾脏单个核细胞内用冷酚法提取了大量富含IL-2mRNA的胞浆RNA。用制备型柱状7M尿素—SDS—PAGE分离胞浆RNA,并结合麦胚无细胞转译体系和IL-2生物活性检测,测定出人IL-2mRNA全长片段约为14S。将转译IL-2(tIL-2)与基因工程IL-2(rIL-2)和部分纯化的天然IL-2(nIL-2)进行分子筛层析,证实三种IL-2洗脱峰基本相同,分子量约为14000道尔顿。tIL-2与IL-2R具良好的结合特性。

Experiments were conducted in intact and adrenalectomized female Wistarrats given dexamethasone,deoxycorticosterone or both,and atria total RNA wasextracted with cold phenol method and hybridized to rat ANP-cDNA probe labeledwith α-~32P.The ANP gene transcripts in intact rats receiving dexamethasoneand in adrenalectomized rats receiving dexamethasone and deoxycorticosterone in-creased by 2-fold,while there were no significant changes in the other groups.Theresults suggest that glucocorticoids enhance ANP gene...

Experiments were conducted in intact and adrenalectomized female Wistarrats given dexamethasone,deoxycorticosterone or both,and atria total RNA wasextracted with cold phenol method and hybridized to rat ANP-cDNA probe labeledwith α-~32P.The ANP gene transcripts in intact rats receiving dexamethasoneand in adrenalectomized rats receiving dexamethasone and deoxycorticosterone in-creased by 2-fold,while there were no significant changes in the other groups.Theresults suggest that glucocorticoids enhance ANP gene expression,an effect relyingon the presence of mineralocorticoids which given alone does not give rise to ANPgene expression enhancement.

给完整的及切除肾上腺的雌性 Wistar 大鼠分別注射地塞米松、去氧皮质酮或地塞米松加去氧皮质酮;冷酚法提取心房总 RNA,用α-~(32P)标记的大鼠心房肽 cDNA 探针与之杂交。完整大鼠接受地塞米松和切除肾上腺后接受地塞米松加去氧皮质酮的大鼠,心房肽基因转录产物增加2倍,其余组无显著变化。结果提示糖皮质激素可促进心房肽基因表达,但此作用依赖于盐皮质激素的同时存在,单纯盐皮质激素不能增强该基因的表达。

 
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