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完整编码
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  complete coding
     A 3.76 kb genome DNA fragment, containing the complete coding sequences of UL31, UL32, UL33 and UL34 genes and the partial coding sequences of UL30 and UL35 genes of Pseudorabies virus (PRV) Ea strain, was amplified by PCR and sequenced.
     从伪狂犬病病毒Ea株基因组DNA中扩增到3.76 kb的基因组片段,该片段包含UL31、UL32、UL33和UL34基因完整编码区,以及UL30和UL35基因部分序列。
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     The sequence of the porcine MEF2C gene cDNA was 1940-bp which contained the complete coding region, and the porcine CACNG1 gene was 1136bp in length, respectively.
     获得了包括完整编码区的长度为1940bp的MEF2C基因和长度为1136bp的CACNG1基因的cDNA序列; 通过RACE得到了猪MEF2B基因的5'非翻译区序列,将该序列与PCR扩增的产物拼接后得到了长度大约为1070bp的猪MEF2B基因cDNA序列。
短句来源
     CRLP(Crn-like Protein) is a novel gene of complete coding sequence that is isolated from the human placenta cDNA library by a high throughput cDNA transfection screening assay established by our laboratory.
     CRLP(Crn-like Protein)是通过我室建立的高通量cDNA转染技术从人类胎盘cDNA文库中筛选出的具有完整编码序列的新基因。
短句来源
     The sequence showed that Prp gene of musk deer was 771 bp long, including complete coding region of Prp gene, which was a complete ORF contained within a single exon, and was basically same as the published gene sequences of animals in same family.
     序列分析表明所克隆的马麝PrP基因片段大约为771bp,包含了朊蛋白基因的完整编码区序列,即包含在单一外显子内的完整开放阅读框,与国外报道的同科动物PrP基因序列基本相同。
短句来源
     Methods Human A NNEXIN A 2 complete coding region was amplified by RT-PCR method from human normal lung tissues, and was reconstructed into the pcDNA3.1/TOPO(?)
     方法采用逆转录聚合酶链式反应技术,从人类正常肺组织中扩增出人类ANNEXIN A2基因的完整编码区全长序列,通过DNA重组技术将该基因重组于pcDNA3.1/TOPO(?)
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  “完整编码”译为未确定词的双语例句
     The entire ERβcoding sequence was then cloned into pcDNA3-HA,resulting in the pcDNA3-HA-ERβrecombinant plasmid.
     再将ERβ完整编码区序列克隆到pcDNA3-HA中,得到重组质粒pcDNA3-HA-ERβ。
短句来源
     According to the 3AB gene of FMDV, the primers of 3AB gene was designed, and the objective fragment was 528 bp which was cloned into pMD18-T vector.
     根据中国口蹄疫流行毒株设计1对包含3AB基因完整编码区的特异性引物,扩增出3AB基因528 bp的特异带,并将纯化的扩增产物克隆到pMD18-T载体上。
     Methods:The full length L1 coden region of HPV58 was amplified by PCR,and cloned into pUC19,sequenced.
     方法 :用聚合酶链反应 (PCR)扩增HPV58L1完整编码区基因 ,将PCR扩增产物克隆至 pUC1 9质粒中并测序。
短句来源
     Nucleotide sequence analysis indicated that the fragment has 162 nt coding for transit peptides (TP) and 21 nt coding for DHDPS amino end of mature protein.
     总DNA为模板,PCR扩增DHDPS叶绿体转运肽的编码序列,序列分析结果显示,扩增片段中含有DHDPS转运肽完整编码区162个核苷酸及成熟蛋白N-端编码区21个核苷酸。
短句来源
     3. Combining the in silico cloning, RACE and RT-PCR method, the full-length cDNA sequence of PSMC1, PSMC4 and PSMC5 gene and the entire coding region of the PSMC2 and PSMD4 genes were cloned.
     3.利用电脑克隆、RACE和RT-PCR的方法相结合,克隆了蛋白酶体激活因子PA700相关基因PSMC1、PSMC4和PSMC5共3个基因的全长cDNA序列,以及PSMC2和PSMD4基因的完整编码区(coding sequence,CDS)。 进行了蛋白质序列的推导和功能域的预测。
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  相似匹配句对
     An Integrated Huffman Code System
     用C实现完整的哈夫曼编码系统
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     3. entropy coding.
     熵编码
短句来源
     texture coding;
     纹理编码 ;
短句来源
     The cellular ultrastructures were also intacted.
     细胞结构完整 .
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     the tonoplast structure remained intact;
     液泡膜完整
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  complete coding
A cDNA fragment containing the complete coding part of the gene was isolated on the basis of the known N-terminal amino acid sequence of the mature form of the trypsin.
      
It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis.
      
Using RT-PCR, we have amplified the complete coding sequence for Pγ from mouse lung RNA.
      
In this study, we cloned and sequenced goat ABCG2 gene complete coding sequence and predicted its putative translated protein structure with implicative functional domains.
      
A cDNA clone (pTag1436) carrying a complete coding sequence for a γ-gliadin polypeptide has been identified and sequenced.
      
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The expression vector λgTllAmp3 has been used to constructa cDNA library from rat liver mRNA.Twenty independent clones,expres-sing antigenic determinants to rat albumin;were isolated.The sizes of the cDNAinsertrons for these clones varied from 0.9 to 1.9 kb.Three of them(clone 4,12,21)were further identified by restriction mapping and DNA sequencing.The lar-gest insertron contained the entire coding sequence for mature albumin.Observa-tion of the expressed proteins by polyacrylamide gel electrophoresis followed...

The expression vector λgTllAmp3 has been used to constructa cDNA library from rat liver mRNA.Twenty independent clones,expres-sing antigenic determinants to rat albumin;were isolated.The sizes of the cDNAinsertrons for these clones varied from 0.9 to 1.9 kb.Three of them(clone 4,12,21)were further identified by restriction mapping and DNA sequencing.The lar-gest insertron contained the entire coding sequence for mature albumin.Observa-tion of the expressed proteins by polyacrylamide gel electrophoresis followed byimmunological detection indicated that the proteins were produced as hybrids lin-ked to the bacterial β-galactosidase.The ~(32)P-labelled cDNA for albumin was used to measure mRNA levels by DNA-RNA hybridization,During acute inflammation,the level of the albuminmRNA in ratliver decreased,reaching the minimum of 25% of the normal level36h after inducing inflammation.

利用表达载体λgT11Amp3以大鼠肝 mRNA 建立了一个 cDNA 文库,从中分离了表达大鼠血清白蛋白抗原决定簇的克隆。内切酶作用图谱和 DNA 顺序测定法表明最大的插入子含有成熟白蛋白的完整编码顺序。聚丙烯酰胺凝胶电泳和免疫检测表明所表达的蛋白质是和细菌β-半乳糖苷酶一起以融合蛋白质的形式存在的。以 DNA-RNA 杂交法用~(32)P 标记的血清白蛋白 cDNA 进行测定,表明在急性炎症期间,大鼠肝中的白蛋白 mRNA 水平大大下降。

The complete encoding sequence with the initiation and stop codons ofMarek's disease virus(MDV) 38 kd phosphorylated protein (pp38) gene wasintegrated into the baculovirus AcNPV transfer vector pVL1392. The insectcell line Sf9 cells were cotransfected with the recombinant transfer vectorpVLpp38 I and the wild type AcNPV DNA. A recombinant baculovirus cloneBP38 I, which was expressing the MDV pp38 gene, was screened with theanti-MDV monoclonal antibody (MAb) H19 in fluorescence antibody test.Immunoblot indicates...

The complete encoding sequence with the initiation and stop codons ofMarek's disease virus(MDV) 38 kd phosphorylated protein (pp38) gene wasintegrated into the baculovirus AcNPV transfer vector pVL1392. The insectcell line Sf9 cells were cotransfected with the recombinant transfer vectorpVLpp38 I and the wild type AcNPV DNA. A recombinant baculovirus cloneBP38 I, which was expressing the MDV pp38 gene, was screened with theanti-MDV monoclonal antibody (MAb) H19 in fluorescence antibody test.Immunoblot indicates that the MAb H19 could recognize a MDV-specific pro-tein band of 35--36 kd from the recombinant virus BP38 I -infected Sf9 celllysates.

鸡马立克病病毒(MDV)38kd磷蛋白(pp38)基因中包括起始密码子和终止密码子的完整编码序列被整合进杆状病毒AcNPV的转移载体质粒pVL1392,用所得的含pp38基因的重组转移载体质粒pVLpp38I与野生型杆状病毒AcNPV的DNA共转染昆虫传代细胞系Sf9细胞后,用荧光抗体法以抗MDV单克隆抗体H_(19)筛选到能表达MDVpp38的重组杆状病毒克隆BP38 I。免疫印迹试验表明,在重组病毒BP38 I感染的Sf9细胞溶解物中,可表现一条分子量约为35—36kd的为单克隆抗体H_(19)识别的MDV特异性蛋白带。

wo oligonucleotide primers were synthesized according to the reported cDNA sequence ofRice Dwarf Virus (RDV)genome segment S6 - Japanese isolsate for the purpose of obatiningthe coding sequence of RDV S6 from the virus of the Fujian isolate.cDNA synthesis andPCR were carried out by using these primers. The PCR product was cloned to the plasmidvector pGEM3Zf(一)and the recombinant DNA was identified by restriction mapping andDNA sequencing analysis.The cloned fragment is 1579 bp long and contains a single openreading...

wo oligonucleotide primers were synthesized according to the reported cDNA sequence ofRice Dwarf Virus (RDV)genome segment S6 - Japanese isolsate for the purpose of obatiningthe coding sequence of RDV S6 from the virus of the Fujian isolate.cDNA synthesis andPCR were carried out by using these primers. The PCR product was cloned to the plasmidvector pGEM3Zf(一)and the recombinant DNA was identified by restriction mapping andDNA sequencing analysis.The cloned fragment is 1579 bp long and contains a single openreading frame of RDV S6.Four subclones were prepared and then sequenced by Sangerdideoxy-mediated chain-termination method.Comparisons of the nucleotide sequences anddeduced amino acid sequences between two RDV S6 of different isolates showed the identitiesof 96.7% and 98.8% respectively.

从水稻矮缩病毒(RiceDwarfVirus,RDV)中国福建分离物的基因组中分离出第六号   片段的双链RNA,根据已发表的日本分离物第六号片段cDNA全序列合成了两个寡聚核苷酸引物,经过逆转录合成cDNA,应用PCR方法扩增了编码区的基因序列,并将扩增产物克隆到pGEM3Zf(一)载体上。对重组子进行的限制性酶切分析和DNA序列分析证明,得到的是RDV第六号片段完整的编码序列,进而构建了亚克隆并进行了全序列测定。结果表明,我们所扩增和克隆的片段长1579bp,包含了第六号片段的长153Obp的阅读框架部分,编码一个由509个氨基酸组成的蛋白质,与日本分离物相应序列相比较有较高的同源性,核苷酸序列及由此推算出的氨基酸序列的同源率分别为96.7%和98.8%。

 
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