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  complete coding
     From one blood donor serum, two SENV-H isolates named SENV-H12-1 and SENV-H 12-2 spanning the complete coding region were amplified by nested PCR respectively.
     从一例健康人血清中分段克隆得到了两个SENV-H亚型分离株(SENV-H12-1和SENV-H12-2)的全部编码区基因序列。
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     METHODS The complete coding region of the two genes were amplified with polymerase chain reaction (PCR), and bidirectional sequencing of the PCR products was subsequently applied in the 36 family members to identify the possible mutations or polymorphisms in the candidate genes.
     方法对一个6代相传、全基因组扫描连锁分析定位于DFNA4座位的常染色体显性遗传性耳聋中国家系成员,针对候选基因KCNN4、KPTN的全部编码序列设计引物,应用PCR扩增反应、产物纯化后直接测序的方法进行突变或多态性位点的检测与鉴定。
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     the product was cloned and sequenced. After that,the DNA sequences were sequenced using BLASTN soft. (l)The complete coding sequences including exon land exon 2 of pig BMP15 were obtained while they were submitted to GenBank (Accession Number, Large White: AH012831,Mei Shan: AH012830).
     (1)获得包含猪BMP15基因外显子1(exon1)和外显子2(exon2)的全部编码区序列,并提交于GenBank,得到猪BMP15基因编码区序列的登录号分别为:AY283024、AY295073、AH012831、AY283025、AY295074、AH012830。
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     A cDNA fragment containing the complete coding region for human p27 was prepared by polymerase chain reaction and ligated efficiently with the linearized expression vector pCRTM3 through a new cloning technique of PCR productsEukaryotic TA Cloning System.
     作者通过PCR方法获得含人p27全部编码区域的p27cDNA片段,并用PCR产物TA克隆法,将其构建在真核表达载体pCRTM3中;
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     Methods Genetic DNA was prepared from peripheral blood leukocytes using DNA Isolation Kits for Mammalian Blood. The complete coding region and exon-intron splice sites of CNGA1 gene were amplified with polymerase chain reaction (PCR).
     方法采集患者外周血,应用DNA分离试剂盒提取DNA,应用11对CNGA1基因引物进行聚合酶链反应(polymerasechainreaction,PCR),扩增CNGA1基因的全部编码区及内含子外显子的拼接区。
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  “全部编码”译为未确定词的双语例句
     Nonstructural proteins NS2 and NS3 are present as diad NS2-3 in wildtype noncytopathic CSFV. NS2 gene is deleted together with all upstream coding regions in CSFV DI particles, and diad NS2-3 is changed to NS3 monomer.
     野生型CSFV非结构蛋白NS2和NS3以二联体形式NS2-3存在,而在干扰缺损颗粒中,NS2基因连同上游的全部编码区被缺失,NS2-3二联体变成NS3单体。
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     Methods By PCR SSCP analysis, we examined 627 bp of exon 28 and 31 of NF1 gene in Chinese patients with NF1, which accounted for 6.95% of NF1 total coding region.
     方法 用PCR SSCP方法检查NF1基因 2 8和 3 1号外显子共约 10 60bp ,约占NF1基因全部编码区的 6 95 %。
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     (2)There were 4 variations in the coding region of apMl:SNP45 in exon 2;
     (2)apMl基因全部编码区上共发现4种单核苷酸变异:2号外显子内SNP45和3号外显子内H142P、R22lS及H241 P。
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     Detection of single nucletide polymorphism of all coding regions in ABCA1 gene in patients with coronary heart disease
     冠心病患者ABCA1基因全部编码区SNP的检测及意义
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     Results The result of PBV220 IL 18cDNA sequencing showed that the sequence accorded with that published completely, and restriction enzyme degestion suggested that a fragment of about 470 bp was subcloned into PGEX 4T 3.Conclusion The gene encoding full human IL 18 active protein was obtained.
     结果测序结果表明克隆至PBV220中的IL-18cDNA包含成熟IL-18蛋白的全部编码序列,酶切分析表明一约470bp的基因片段克隆至PGEX-4T-3中,与理论预计的一致。 结论获得了编码人IL-18成熟活性蛋白的cDNA克隆。
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     All of then were cured.
     全部治愈。
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     3. entropy coding.
     熵编码
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     1 encoding for RAS and call signalling messages.
     1编码;
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     All 4 hot spots of mutation in VP_1 gene led to mutations of deduced amino acid.
     4个突变热点全部导致了编码氨基酸的突变。
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     all effective.
     全部有效。
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  complete coding
A cDNA fragment containing the complete coding part of the gene was isolated on the basis of the known N-terminal amino acid sequence of the mature form of the trypsin.
      
It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis.
      
Using RT-PCR, we have amplified the complete coding sequence for Pγ from mouse lung RNA.
      
In this study, we cloned and sequenced goat ABCG2 gene complete coding sequence and predicted its putative translated protein structure with implicative functional domains.
      
A cDNA clone (pTag1436) carrying a complete coding sequence for a γ-gliadin polypeptide has been identified and sequenced.
      
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Using the shutgun strategy and cloning of DNA restriction fragments technology,the sequence of HBcAg gene,adr subtype,was determined by dideoxy method and labeled with a35S dATP as radioactive marker.The results indicated that the adr NC-1 core antigen gene is composed of 552 mucleotides from codon ATG to TAG and encoding the polypeptide of 183 amino acids with a molecular weight of 21000 dlt.In comparison with the data published in Japan and Shanghai,there are 1.3-1.8% nucleotides change between adrNC-1 and...

Using the shutgun strategy and cloning of DNA restriction fragments technology,the sequence of HBcAg gene,adr subtype,was determined by dideoxy method and labeled with a35S dATP as radioactive marker.The results indicated that the adr NC-1 core antigen gene is composed of 552 mucleotides from codon ATG to TAG and encoding the polypeptide of 183 amino acids with a molecular weight of 21000 dlt.In comparison with the data published in Japan and Shanghai,there are 1.3-1.8% nucleotides change between adrNC-1 and other adr subtypes corresponding to 0.5% missense mutation,and 9.8-10.9% nucleotides change between adrNC-1 and adw ayw with 3.3-4.4% missense mutation.

采用定点克隆与随机克隆相结合的实验设计,用α~(35)S-dATP标记,以末端终止法测定了adr亚型乙型肝炎病毒DNA核心抗原基因的全顺序,包括终止密码子在内基因全长552核苷酸,编码由183氨基酸组成的多肽,计算分子量21000dlt。与日本及上海等实验室报道的adr亚型HBcAg基因比较,核苷酸点突变1.3—1.8%,由点突变导致的错义氨基酸突变有一处,占全部编码氨基酸的0.5%。与adw及ayw亚型比较,核苷酸点突变占全部核苷酸的9.8—10.9%,导致的错义突变占全部编码氨基酸的3.3—4.4%,并对eAg基因的定位进行了讨论。

The psbA gene cloned in pSB135 from Solatium nigrum atrazine-resistant biotype was se-quenced.It has the same nuclcotide sequence as the known atrazine resistance gene from another independent biotype of S.nigrum.On the basis of the deduced amino acid sequences the secondary structure of the 32kD proteins encoded by the pfbA genes were compared between atrazine-resistant and susceptible biotypes,and some indications from the protein structure comparison were discussed.

对克隆在psb135质粒上的来自龙葵阿特拉津抗性生物型的psbA基因进行DNA序列分析,测得长为1384个核苷酸的全序列,包括该基因的全部编码区和5′上游顺序。该基因的核苷酸序列与另一个独立来源的龙葵阿特拉津抗性基因的核苷酸序列完全相同,在由核苷酸推导的氨基酸序列的基础上,比较了分别由龙葵抗阿特拉津和对阿特拉津敏感的psbA基因编码的32kD蛋白质的二级结构,并对其可能的含意进行了讨论。

The cDNA for human G-CSF was cloned from total RNA of human bladder carcinoma 5637 cells following PCR amplification. Sequencing data proved that the cloned cDNA contained the intact coding region of human G-CSF gene which was 612 bp in length, encoding 30 amino acids of signal peptide and 174 amino acids of the mature protein. One base mutation was found in the 43rd codon of the cDNA (CAC→TAC) resulting in the replacement of histidine by tyrosine. The gene products proved having G-CSF aetivity after its preliminary...

The cDNA for human G-CSF was cloned from total RNA of human bladder carcinoma 5637 cells following PCR amplification. Sequencing data proved that the cloned cDNA contained the intact coding region of human G-CSF gene which was 612 bp in length, encoding 30 amino acids of signal peptide and 174 amino acids of the mature protein. One base mutation was found in the 43rd codon of the cDNA (CAC→TAC) resulting in the replacement of histidine by tyrosine. The gene products proved having G-CSF aetivity after its preliminary expression in SP2/0 cells following retroviral transfer.

利用多聚酶链反应技术,从人膀胱癌细胞系5637细胞中快速扩增并克隆了人粒细胞集落刺激因子cDNA,序列分析证明,该cDNA包含人粒细胞集落刺激因子的全部编码基因,全长612bp,编码30个氨基酸的信号肽和174个氨基酸的成熟蛋白。其中第43位codon出现一个碱基的突变(CAC→TAC)导至第43位氨基酸的改变(组氨酸→酪氨酸)。经逆转录病毒导入SP2/0细胞并初步表达。结果表明:该基因产物具有G-CSF活性。

 
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