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   全长编码 的翻译结果: 查询用时:0.487秒
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全长编码
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  complete coding
     Methods: Complete coding BMP-2 gene sequences were obtained from Genebank. Human BMP-2 gene, mouse BMP-2 gene, and chicken BMP-2 gene were analyzed with firstEF, footprinter and Consite software.
     方法:从Genebank中获得BMP-2的全长编码基因,利用firstEF,footprinter和Consite软件对人类BMP-2基因,小鼠BMP-2基因,鸡BMP-2基因的5’侧翼序列进行生物信息学分析。
短句来源
     AIM: To clone the complete coding sequence of mouse oxytocin receptor (mOTR) gene and to express it in mammalian cells.
     目的 :为了获得小鼠催产素受体 (mouseoxytocinreceptor,mOTR)基因的全长编码区和构建mOTR的真核表达载体 ,以及在哺乳动物细胞中真核表达mOTR。
短句来源
     Complete coding sequences of β arrestin1 (1A and 1B) were cloned through application of bioinformatics analysis to the dbEST database.
     用生物信息法获得了人类βarestin1两个亚型(1A和1B)的全长编码序列的cDNA。
短句来源
     As a result,we confirmed that one clone contained a 1.3 kb cDNA insert including the complete coding region of human interleukin 6,and the other 0.9 kb insert lacking the area encoding the signal peptide and thirty amino terminal residures of mature IL-6.
     结果证明,一个克隆的cDNA片段长1.3kb,含有人白介素6全长编码区; 另一个的cDNA插入片段为0.9kb,缺乏信号肽及成熟IL-6N端30个残基的编码序列。
短句来源
     Conventionally there are three conditions for obtaining complete coding genetic sequence of schistosoma japonicum,the gene may has been known,partial known or unknown.
     通常取日本血吸虫全长编码序列会面对以下三种情况 :其因可能为已知序列的基因 ,已知部分序列的基因或者不知序列的基因 ,本文针对以上常见的每一种情况。
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  “全长编码”译为未确定词的双语例句
     [Methods] After the complete coded gene of the human OPG cDNA was amplified and cloned into the mammalia vector pcDNA3.1/CT-GFP,the recombination OPG expressing vector pcDNA3.1/OPG-GFPhas been constructed.
     方法用RT-PCR方法将人OPG cDNA的全长编码基因扩增后,将其克隆于哺乳动物表达载体pcDNA 3.1/CT-GFP上,构建OPG的重组表达载体pcDNA 3.1/OPG-GFP。
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     According to the cDNA sequence of human HSP70D, we designed primers to amplify full-length code region of human HSP70D.
     首先,根据人HSP70D cDNA序列设计一对包含HSP70D全长编码区的PCR引物。
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     Methods: The plasmid pPCIcc containing the full length of LMP2A cDNA was digested with EcoR I and Not I and cloned into expression vector pPICZαA.
     方法:用EcoR I和Not I将含LMP2A全长编码基因的质粒PCIcc双酶切后,克隆到毕氏酵母载体PICZαA中,构建重组表达质粒pPICZαA-LMP2A。
短句来源
     The RT-PCR product of specimens positive for MAGE-4 was sequenced and proved as the full length of MAGE-4 gene.
     对阳性标本的RT-PCR扩增产物中目的基因片段进行DNA测序,证实为MAGE-4全长编码基因。
短句来源
     Chinese hamster ovary (CHO) cells were transiently cotransfected with plasmids harboring the entire coding region of GPⅠbα, GPⅠβ and GPⅨ or mutant GPⅨ, respectively.
     用含有GPⅠbα ,GPⅠbβ和GPⅨ或GPⅨ突变型全长编码序列的质粒对中国仓鼠卵巢 (CHO)细胞共转染 ;
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  相似匹配句对
     Summary of the methods for obtaining complete coding genetic sequences of schistosoma japonicum
     血吸虫全长编码基因序列获取方法概述
短句来源
     It contains 1632bp long from the ATG to the stop codon, encoding a predicated protein of 60kD and is composed of 544 amino acids.
     其全长1993bp,编码区长1632bp。
短句来源
     3. entropy coding.
     熵编码
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     1 encoding for RAS and call signalling messages.
     1编码;
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  complete coding
A cDNA fragment containing the complete coding part of the gene was isolated on the basis of the known N-terminal amino acid sequence of the mature form of the trypsin.
      
It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis.
      
Using RT-PCR, we have amplified the complete coding sequence for Pγ from mouse lung RNA.
      
In this study, we cloned and sequenced goat ABCG2 gene complete coding sequence and predicted its putative translated protein structure with implicative functional domains.
      
A cDNA clone (pTag1436) carrying a complete coding sequence for a γ-gliadin polypeptide has been identified and sequenced.
      
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From monolayer of He La cells treated with recombinant human interferon alpha,total poly(A)+RNA was routinely extracted,which was used as template for synthesis of the first strand of cDNA by reverse transcriptase.A cDNA library was constucted in phagemid vector pTZigR by the primer-adapter procedure.Six positive clones were screened out of this library by means of colony and slot-blotting hybridizations using 32P-labled IL-6 fragment of 480 bp as a probe.Two of them were further characterized by restriction...

From monolayer of He La cells treated with recombinant human interferon alpha,total poly(A)+RNA was routinely extracted,which was used as template for synthesis of the first strand of cDNA by reverse transcriptase.A cDNA library was constucted in phagemid vector pTZigR by the primer-adapter procedure.Six positive clones were screened out of this library by means of colony and slot-blotting hybridizations using 32P-labled IL-6 fragment of 480 bp as a probe.Two of them were further characterized by restriction analysis and DNA sequencing.As a result,we confirmed that one clone contained a 1.3 kb cDNA insert including the complete coding region of human interleukin 6,and the other 0.9 kb insert lacking the area encoding the signal peptide and thirty amino terminal residures of mature IL-6.

我们从重组的人α干扰素处理的单层HeLa细胞常规提取Poly(A)~+RNA作为逆转录合成cDNA第一链之模板,用引物-适配接头法在噬菌质粒pTz19R中构建cDNA文库。以~(32)p-标记的480bpIL-6cDNA片段作探针进行菌落原位及狭缝印迹杂交,筛选出6个阳性克隆。其中两个克隆并用限制酶切分析及DNA序列测定做进一步鉴定。结果证明,一个克隆的cDNA片段长1.3kb,含有人白介素6全长编码区;另一个的cDNA插入片段为0.9kb,缺乏信号肽及成熟IL-6N端30个残基的编码序列。

Transformation of the re-combinant plasmid pRK41 containing 2.15kd human brain myelin basic proteinMBP)-coding sequence and 3' untrans-lated region(1.2kb) into the E.coli JM109was made by using Hanahan's method.Positive colonies were sereened withdigoxigenin oligo labelled rat brain MBPcDNA fragment (1.2kb). To remove 3'untranslated region and obtain the full-length coding sequence of MBP cDNA. apair of specific DNA primers was desig-ned and synthesized. A 600 bp fragmentwas amplified from the recombinant plas-mid,...

Transformation of the re-combinant plasmid pRK41 containing 2.15kd human brain myelin basic proteinMBP)-coding sequence and 3' untrans-lated region(1.2kb) into the E.coli JM109was made by using Hanahan's method.Positive colonies were sereened withdigoxigenin oligo labelled rat brain MBPcDNA fragment (1.2kb). To remove 3'untranslated region and obtain the full-length coding sequence of MBP cDNA. apair of specific DNA primers was desig-ned and synthesized. A 600 bp fragmentwas amplified from the recombinant plas-mid, extracted from the positive colonyby using polymerase chain reaction(PCR).The PCR fragment was isolated, and thendigested with BamH I, Kpn I and BamHI+Kpn I .The results of restriction ana-lysis indicate that the PCR amplifiedfragment is desirable and can be useddirectly to constract expression vectors.

作者将含人脑髓鞘碱性蛋白(MBP)编码区和3’端非翻译区(1.2kb)的重组质粒pRK41—1.2转化宿主菌E.coli JM109,用非同位素标记鼠MBP cDNA克隆片段作探针,经菌落原位杂交筛选出阳性克隆并以此为模板,采用聚合酶链反应(PCR)技术扩增出含21.5kd MBP全长编码序列(600bp),又经限制性内切酶BamHⅠ和KpnⅠ消化,电泳结果进一步证实该PCR产物的特异性,为人MBP全长编码序列。

series of DNA primers specific for humanbrain myelin basic protein (MBP) gene wasdesigned and synthesized. MBP cDNA frag-ment about 600bp in length was amplified fromhuman brain cDNA library by using poly-merase chain reaction (PCR) with the specificprimers P_1 and P_2. The recovered PCR productwas flushed by klenow fragment and insertedinto pGEM-3Zf (+ ) vector pretreated withSmaⅠand calf intestinal alkaline phophatase.The recombinant plasmid was used to trans-form competent cell JM 109. The positivecolonies...

series of DNA primers specific for humanbrain myelin basic protein (MBP) gene wasdesigned and synthesized. MBP cDNA frag-ment about 600bp in length was amplified fromhuman brain cDNA library by using poly-merase chain reaction (PCR) with the specificprimers P_1 and P_2. The recovered PCR productwas flushed by klenow fragment and insertedinto pGEM-3Zf (+ ) vector pretreated withSmaⅠand calf intestinal alkaline phophatase.The recombinant plasmid was used to trans-form competent cell JM 109. The positivecolonies were directly screened on indicatorplates.The recombinant plasmid DNA and in-sert fragment isolated from four positivecolonies were analyzed by digestion with EcoRⅠ, Kpn Ⅰand Taq Ⅰ.The different codingsequences including MBP exon Ⅰ─Ⅶ.Ⅰ─Ⅲ,Ⅲ─Ⅶ and Ⅰ─Ⅴ were amplified fromthese clones with their corresponding nestedsets of primers respectively. These resultsshow that these cDNA clones contain full-length coding sequence for 21.5kD humanMBP.

采用聚合酶链反应(PCR)从人脑cDNA文库中扩增出600bp的髓鞘碱性蛋白(MBP)cDNA片段,与载体pGEM-3Zf(+)平端连接.重组质粒DNA转化宿主菌JM109,在含X-gal和IPTG的平板上直接筛选阳性克隆.限制性内切酶分析和成套引物扩增鉴定证明,该克隆含有7个外显子的21.5kD人脑MBP全长编码序列.

 
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