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胞浆空泡内
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  cytoplasmic vacuoles
     The mature DEV which had the structures of envelop and tegument was spherical in shape with diameter of 150-300 nm and located in cytoplasmic vacuoles.
     成熟的病毒粒子具有囊膜和皮层结构,呈圆形,直径150~300 nm,存在于胞浆空泡内;
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  相似匹配句对
     Vacuoles in cytoplasm could be seen but also organellae and microvilli on the cellular membrane of Human.
     胞浆空泡,但仍有细胞器存在;
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     There were swollen mitochondria vacuoles in cytoplasma 3 hours after reperfusion.
     再灌3h胞浆可见肿大的线粒体和空泡变。
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     Oharacteristics of AC3 included marked vacuolations in its cytoplasm and different sized nuclei;
     AC3最显著的特征是胞浆广泛分布的脂质空泡
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     The cytosolic PKA activity was increased.
     胞浆 PKA活性升高。
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     3) Inter cytoplasmicglycogen increase, occasional sightings of small amounts of glocogen inthe nucleus;
     3)胞浆糖原增多,空泡变性,核偶见少量糖原;
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  cytoplasmic vacuoles
In addition, these observations showed that salt ions are localized in the central vacuole of leaf cells (via pinocytosis) and also in small cytoplasmic vacuoles of leaf and root cells.
      
During separation of sister nuclei, the "excessive" cytobionts are released into the cytoplasmic vacuoles formed by external membranes of the nuclear envelope.
      
Histologically the tumor is characterized by proliferation of plump epithelial-like endothelial cells with the vascular differentiation being mostly expressed at a cellular level in the form of cytoplasmic vacuoles.
      
Remarkable interstitial edema and cytoplasmic vacuoles in acinar cells were the earliest histologic alterations.
      
The formation of cytoplasmic vacuoles of neutrophilic granulocytes and monocytes after ingestion of latex particles dependent on inflammations of different etiology had been studied in vitro.
      
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Ultrathin sectioning and transmission electron microscopy of infected duck embryo fibroblasts(DEF) were employed to investigate the morphology of virulent duck enteritis virus(DEV) strain CH.The nucleic acid of DEV was round in shape with diameter of 35-45 nm and was often in a cluster in the nucleus of DEF.The nucleocapsid of DEV was round in shape with diameter of 90-100 nm and could be observed both in nucleus and cytoplasm of DEF.Based on their mophological characteristics,DEV nucleocapsids could be divided...

Ultrathin sectioning and transmission electron microscopy of infected duck embryo fibroblasts(DEF) were employed to investigate the morphology of virulent duck enteritis virus(DEV) strain CH.The nucleic acid of DEV was round in shape with diameter of 35-45 nm and was often in a cluster in the nucleus of DEF.The nucleocapsid of DEV was round in shape with diameter of 90-100 nm and could be observed both in nucleus and cytoplasm of DEF.Based on their mophological characteristics,DEV nucleocapsids could be divided into 4 distinct groups which were the hollow nucleocapsid,the nucleocapsid with several nucleic acid particles closely attached to its inner wall,the concentric circle nucleocapsid and the infarctate nucleocapsid.The mature DEV which had the structures of envelop and tegument was spherical in shape with diameter of 150-300 nm and located in cytoplasmic vacuoles.Intracytoplasmic inclusion bodies and intranuclear inclusion bodies could be observed respectively in the cytoplasm and nucleus of infected DEF.With the appearance of progeny DEV in DEF,some densely electron-stained virus-related structures which were rod-shaped,U-shaped,or of circle,semicircle or concentric circle in appearance could be observed in the cytoplasm of DEF.

通过超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒(DEV)CH强毒株在鸭胚成纤维细胞(DEF)中的形态结构进行了研究。结果发现,DEV CH强毒株病毒核酸呈圆形颗粒状,直径35~45 nm,在胞核内常集中分布;病毒核衣壳呈圆形,直径90~100 nm,在胞核和胞浆内都有分布;DEV核衣壳可根据所含核酸形态的差异分为空心核衣壳、内壁附有颗粒型核衣壳、同心圆形核衣壳和实心核衣壳;成熟的病毒粒子具有囊膜和皮层结构,呈圆形,直径150~300 nm,存在于胞浆空泡内;DEV可在DEF中分别形成胞浆内和胞核内包涵体结构;伴随子代病毒在细胞内的出现,胞浆内还出现豆荚状、马蹄形、半圆形、圆形、同心圆形等与病毒发生有关的电子致密结构。

Objective To determine whether Acanthamoeba polyphaga could affect the survival and growth of Vibrio cholerae O139 in low temperature. Methods V. cholerae O139 was co-cultured with the Acanthamoeba polyphaga to be examined on its intracellular growth and survival rate within cysts at low temperature, using methods as Gram-staining, electron microscope and passage culture. Results V. cholerae O139 were observed to enter into the trophozoites and grow the within the vacuoles after 8 hour incubation with Acanthamoeba...

Objective To determine whether Acanthamoeba polyphaga could affect the survival and growth of Vibrio cholerae O139 in low temperature. Methods V. cholerae O139 was co-cultured with the Acanthamoeba polyphaga to be examined on its intracellular growth and survival rate within cysts at low temperature, using methods as Gram-staining, electron microscope and passage culture. Results V. cholerae O139 were observed to enter into the trophozoites and grow the within the vacuoles after 8 hour incubation with Acanthamoeba polyphaga. The germs survived in the vacuole and/or endo-layer of wall and could be re-isolated from the excystment of Acanthamoeba polyphaga. At 30℃, V. cholerae O139 could survive for 120 days with the amoeba while less than 45 days in PAS. At 4℃, the number of viable bacteria decreased and reached undetectable levels for both study and control groups after a 30-day incubation. V. cholerae O139 could be re-isolated from the 30-, 45-, 60- and 75- day's infected cysts after excystment. However the ability of excystment for 90- clay's infected cysts decreased and V. cholerae O139 within the cyst could not be isolated again because the amoebae had lysed. Conclusion These findings indicated that V. cholerae O139 could grow within Acanthamoeba polyphaga and the survival time could be increased in the cysts at low temperature. It seemed that Acanthamoeba can provide an environmental reservoir for V. cholerae O139.

目的观察棘阿米巴原虫对霍乱弧菌O139的环境生存影响。方法采用共培养方法, 通过染色和电镜超薄切片来观察霍乱弧菌O139在多噬棘阿米巴的滋养体和包囊内生存情况,经包囊传代及低温下生存耐受实验来观察阿米巴包囊能否提高霍乱弧菌O139的环境生存能力。结果与多噬棘阿米巴共培养8 h后,霍乱弧菌O139就能进入阿米巴滋养体内并在空泡内增殖。霍乱弧菌 O139可生存于胞浆空泡内和(或)包囊内壁间,经包囊传代培养能再分离培养出此菌。在30℃时,在棘阿米巴环境下120 d仍能检测出霍乱弧菌O139,而在阿米巴生理盐水液中45 d后就检测不出霍乱弧菌O139。在4℃时,实验组和对照组培养液30 d后均未能培养出霍乱弧菌O139;而被感染包囊分别在30、45、60和75 d后复苏培养能再检测出霍乱弧菌O139;被感染包囊90 d后发育成滋养体能力下降,未能再培养出活的霍乱弧菌O139,在电镜下可见包囊被裂解死亡。结论霍乱弧菌O139能在棘阿米巴的滋养体内繁殖和包囊内生存;阿米巴包囊能够提高霍乱弧菌O139的低温下生存能力;棘阿米巴包囊可能成为霍乱弧菌O139越冬的环境宿主。

 
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