Transformation and Expression of an Avian Influena A (H5N1) Virus Gene(h5nla) in Transgenic Potato——Compared with Expression of Hepatitis B Surface Antigen(HBsAg) in Transgenic Potato
The authors mainly introduced the progress in the studies on the complexity and the character of gene expression duringand anther and pollen development, anther-and pollen-specific genes, the regulation of the specific genes; and discussedthe prospects for the induction of plant male sterility with a chimeric gene containing the specific gene promoter and areporter gene (for example, RNase gene), and the problems of the application of the method to hybrid cropbreeding.
The recombinant gene containing human proinsulin gene driven by ovine β lactoglobulin gene was microinjected into the pronuclei of goat zygotes for producing transgenic dairy goats.
The three site-mutated human proinsulin gene containing regulatory elements (GLRE3-IGFBP-1P-3mINS) was inserted into the retroviral vector (pLXSN) after restrictive endonuclease digestion, and transfered into rat′s hepatocarcinoma cells CBRH7919 mediated by liposomes. The mature insulin level was detected in the culture media and the expressive difference between GLRE3-IGFBP-1P-3mINS and two site-mutated human proinsulin gene recombinants (GLRE3-IGFBP-1P-2mINS) was compared.
The sGBR gene containing GBR gene that was upstream linked with tissue-type plasminogen activator signal peptide (tPA-SP) was directly cloned into the plasmid pCN-SSIE containing sSBR gene encoding saliva binding region (SBR) of antigen protein Ⅰ/Ⅱ(AgⅠ/Ⅱ) from S.mutans.
A gene regulatory mechanism has been proposed in which steroid hormones and certain other drugs bind to nuclear receptor proteins followed by transfer to DNA where they are inserted between base pairs.
A pair of primers were designed according to published literature on Populus trichocarpa gene (PTD), and PtAP3, an AP3 homologous gene from Populus tomentosa was isolated by PCR using genomic DNA of the male clone of P.
In this study, the promoter fragment of LTF gene containing mutation (G/C) in position +32 has been amplified by PCR followed by genotyping by the SSCP and RFLP method.
A truncated human U6 gene containing convenient restriction sites has been shown to be expressed at high levels following electroporation into a series of human cell lines.
Four different types of cytoplasmic male sterility in maize were tested for their identityby use of the restoration technique.Male-sterile lines incorporated with cytoplasm of theTexas source were found to be completely affected in crosses by a group of restorers includ-ing W153,W28 and G32,and partially restored by W24,M14 and A34.This made a figure of6 restorers out of about 60 inbreds thus tested in the Texas cytoplasm.Inbreds W153 wasselected as a differential restorer for T-type steriles since it gave ...
三种细胞质遗传的玉米雄花不孕类型各有其专效的恢复系和部分恢复系。在 T 型细胞质基础上,测定出两对显性互补基因决定着花粉孕育性的恢复,同时还有显性修饰基因存在,影响其表现的程度。春播和夏播的不同环境条件只对部分恢复性的表现发生明显影响,对全恢复性和不孕性则很少能够改变。M 型恢复性的等位隐性基因在杂合株内对花粉粒具有某种致死或败育作用,故测交后代表现不分离现象。本文初步鉴定了若干常用自交系的 T 型恢复基因型,并以此作为根据,提出了配制全不去雄的玉米双交种的各种可能方案。
The glutamic dehydrogenase(GDH)-positive mutant, B. subtilis IRC-3-N-1, produced alanine dehydrogenase(ADH)when grown in nutrient medium. The formation of ADH by this mutant was inductive in nature. The inducer for ADH synthesis was not restricted to the substrate of the enzyme. Aspartic acid, threonine, both enantiomorphs of alanine and pyruvate were all effective.During the fractionation of the enzymes, GDH and the induced ADH were coexistent in the same fraction and distributed in almost the same proport...
In many important types of reactions involving olefins or acetylenes, catalyzed by compounds of the transition metals, activation of the unsaturated reacting groups is probably effected through the formation of δπ -bonds with the catalysts. In the present article, a critical review of the mechanisms of these important types of teactions is given. The relation between catalysis by coordination activation and the catalytic behaviours of transition metals, oxide semi-conductors and acid catalysts, and the tren...
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