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蚕蜂与野生动物保护
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  gene
     Transformation and Expression of an Avian Influena A (H5N1) Virus Gene(h5nla) in Transgenic Potato——Compared with Expression of Hepatitis B Surface Antigen(HBsAg) in Transgenic Potato
     禽流感病毒(H5N1)基因(h5n1a)在马铃薯中的转化与表达研究——附:乙肝表面抗原(HBsAg)基因转化的对比研究
短句来源
     Cloning and Expression of the Ethylene Receptor Gene (etr1) of Hevea brasiliensis
     巴西橡胶树(Hevea brasiliensis)乙烯受体基因(etr1)的克隆及表达分析
短句来源
     Cloning of Yeast Gene (Pro2) Coding for Proline Synthesizing Enzyme (Glutamate Phosphate Reductase) and Leu~+ Pro~+ Phenotypic Cotransduction
     酵母脯氨酸合成酶基因(Pro2)的分离及Leu~+ Pro~+表型共转导
短句来源
     Experiments of Mammalian Genetic Engineering Experiment 15 DNA Mediated Gene Transfer
     实验15 DNA介导转移基因(DMGT)
短句来源
     SUBCLONING OF THE PROMOTOR FRAGMENT OF THE YEAST 3-PHOSPHOGLYCERATE KINASE GENE (PGK1)
     酿酒酵母3-磷酸甘油酸激酶基因(PGK1)启动子片段的亚克隆
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  gene containing
     The authors mainly introduced the progress in the studies on the complexity and the character of gene expression duringand anther and pollen development, anther-and pollen-specific genes, the regulation of the specific genes; and discussedthe prospects for the induction of plant male sterility with a chimeric gene containing the specific gene promoter and areporter gene (for example, RNase gene), and the problems of the application of the method to hybrid cropbreeding.
     本文着重介绍了花药和花粉发育过程中基因表达的特点和复杂性,花药和花粉的特异性基因以及特异性基因的调节等方面的研究进展,讨论了应用上述特异性基因的调节序列与一定的目的基因(如核糖核酸酶基因)构建的嵌合基因诱导植物雄性不育的前景和这种分子生物学方法在作物杂种优势利用过程中遇到的问题。
短句来源
     The recombinant gene containing human proinsulin gene driven by ovine β lactoglobulin gene was microinjected into the pronucleus of mouse zygotes.
     将构建的绵羊 β-乳球蛋白基因 ( BLG)调控人胰岛素原基因的重组基因通过显微注射生产转基因小鼠。
短句来源
     The recombinant gene containing human proinsulin gene driven by ovine β lactoglobulin gene was microinjected into the pronuclei of goat zygotes for producing transgenic dairy goats.
     将构建的绵羊β-乳球蛋白基因 ( BLG)调控人胰岛素原基因的重组基因通过显微注射生产转基因奶山羊。
短句来源
     The three site-mutated human proinsulin gene containing regulatory elements (GLRE3-IGFBP-1P-3mINS) was inserted into the retroviral vector (pLXSN) after restrictive endonuclease digestion, and transfered into rat′s hepatocarcinoma cells CBRH7919 mediated by liposomes. The mature insulin level was detected in the culture media and the expressive difference between GLRE3-IGFBP-1P-3mINS and two site-mutated human proinsulin gene recombinants (GLRE3-IGFBP-1P-2mINS) was compared.
     经酶切后将含有调控元件的3点突变人胰岛素原基因(GLRE3-IGFBP-1P-3mINS)插入逆转录病毒载体(pLXSN),脂质体介导转染大鼠肝癌细胞CBRH7919后检测成熟胰岛素表达情况及与含有调控元件的2点突变人胰岛素原基因(GLRE3-IGFBP-1P-3mINS)表达体的表达差异。
短句来源
     The sGBR gene containing GBR gene that was upstream linked with tissue-type plasminogen activator signal peptide (tPA-SP) was directly cloned into the plasmid pCN-SSIE containing sSBR gene encoding saliva binding region (SBR) of antigen protein Ⅰ/Ⅱ(AgⅠ/Ⅱ) from S.mutans.
     将合成的组织纤溶酶原信号肽序列(tPA-SP)连接到GBR基因的上游; 再将此基因(sGBR)定向克隆到载有sSBR基因的双启动子真核表达载体pCN-SSIE上,构建出pCN-SSISG;
  “基因(”译为未确定词的双语例句
     Construction of Candida albican calmodulin(CMD1) gene-deleted yeast and study on CMD1 functional base
     白念珠菌钙调蛋白基因(CMD1)缺陷菌株的构建及CMD1功能性基因的初步研究
短句来源
     EXPRESSION OF MDR-1 IN LEUKEMIC CELLS
     多元药物抗性基因(mdr-1)在白血病细胞中的表达
短句来源
     The Natural Selection of a Single Alleles—pair(A,a)
     单对基因(A,a)的自然选择
短句来源
     Lux Genes and Their Application in the Environmental Moniterirg
     荧光素酶基因(Lux)在环境监测中的应用和研究
短句来源
     RADIOSENSITIVITY AND GENES
     辐射敏感性和基因(英文)
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  gene
NEW TECHNOLOGY FOR DRUG DISCOVERY BASED UPON INSERTION OF LIGANDS INTO GENE SEQUENCES BY NUCLEAR RECEPTOR PROTEINS
      
A gene regulatory mechanism has been proposed in which steroid hormones and certain other drugs bind to nuclear receptor proteins followed by transfer to DNA where they are inserted between base pairs.
      
Polymerase chain reaction was used to amplify a 439-bp fragment of a 65,000-kDa (Mr) heat shock protein gene (hsp65) of Mycobacterium.
      
Cloning of an APETALA3 homologous gene (PtAP3) from Populus tomentosa and genetic transformation of its sense and anti-sense con
      
A pair of primers were designed according to published literature on Populus trichocarpa gene (PTD), and PtAP3, an AP3 homologous gene from Populus tomentosa was isolated by PCR using genomic DNA of the male clone of P.
      
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  gene containing
In this study, the promoter fragment of LTF gene containing mutation (G/C) in position +32 has been amplified by PCR followed by genotyping by the SSCP and RFLP method.
      
A senescence-inhibition chimeric gene containing the specific promoter of SAG12 and IPT gene was transferred into rice with the biolistic method.
      
The CD gene containing virus was injected into the tumors.
      
A truncated human U6 gene containing convenient restriction sites has been shown to be expressed at high levels following electroporation into a series of human cell lines.
      
We have demonstrated high level expression of the recombinant U6 gene containing the ribozyme and reduction of Bcl-2 protein in transduced cells.
      
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Four different types of cytoplasmic male sterility in maize were tested for their identityby use of the restoration technique.Male-sterile lines incorporated with cytoplasm of theTexas source were found to be completely affected in crosses by a group of restorers includ-ing W153,W28 and G32,and partially restored by W24,M14 and A34.This made a figure of6 restorers out of about 60 inbreds thus tested in the Texas cytoplasm.Inbreds W153 wasselected as a differential restorer for T-type steriles since it gave no...

Four different types of cytoplasmic male sterility in maize were tested for their identityby use of the restoration technique.Male-sterile lines incorporated with cytoplasm of theTexas source were found to be completely affected in crosses by a group of restorers includ-ing W153,W28 and G32,and partially restored by W24,M14 and A34.This made a figure of6 restorers out of about 60 inbreds thus tested in the Texas cytoplasm.Inbreds W153 wasselected as a differential restorer for T-type steriles since it gave no reactions to the othertypes.For the Moldavia type of male sterility,formerly offered by Prof.Hadjinov,we founda partially restoring inbred W9 to be better suited for the similar purpose.The third andfourth type of male sterility,designated as B- and G-type,came through our own selectionsfrom two Bulgarian varieties.Separation between them seemed difficult.Since A374 gavepartial pollen fertility to the B-type steriles exclusively,it could be used as a differentiat orfor this type of cytoplasm.Pollen restoration in crosses involving W153 and W28 followed the expectation based ona dominant Mendelian gene.However,data obtained from segregating progenies of doublecrosses in which G32 was the restorer suggested strongly that two dominant complementarygenes were more workable.The difference in genotype of inbreds concerned in variousinvestigations seemed to be responsible for such inconsistent results.Segregating patterns in the Texas sterile crosses of the partial restorer,W24 or M14,varied with plants used as the pollen parent and with the date of planting.It appeared verylikely that W24 and M14 were heterozygous for major restorer genes since one sterile versionof W24 and four fully restoring lines of M14 had been established by conversion and test-cross-ing respectively.Dominant modifiers might also be present in either of the inbred popula-tions.In the presence of Moldavian cytoplasm the recessive allele of the restorer gene seemedto exert an abortive action to its pollen carriers produced by the heterozygote.When plantsheterozygous for the restorer gone were outcrossed to male steriles carrying the right cyto-plasm,all plants from the progenies proved to be pollen shedders.It was suggested that theM-type cytoplasm might be similar to that of S-type.Tentative genotypes related to pollen restoration of Texas male sterility had been workedout for a number of inbreds on the basis of two dominant complementary genes.Workingschemes for the production of double-cross seeds of maize without detasseling had been sum-marized and discussed by the authers.

三种细胞质遗传的玉米雄花不孕类型各有其专效的恢复系和部分恢复系。在 T 型细胞质基础上,测定出两对显性互补基因决定着花粉孕育性的恢复,同时还有显性修饰基因存在,影响其表现的程度。春播和夏播的不同环境条件只对部分恢复性的表现发生明显影响,对全恢复性和不孕性则很少能够改变。M 型恢复性的等位隐性基因在杂合株内对花粉粒具有某种致死或败育作用,故测交后代表现不分离现象。本文初步鉴定了若干常用自交系的 T 型恢复基因型,并以此作为根据,提出了配制全不去雄的玉米双交种的各种可能方案。

The glutamic dehydrogenase(GDH)-positive mutant, B. subtilis IRC-3-N-1, produced alanine dehydrogenase(ADH)when grown in nutrient medium. The formation of ADH by this mutant was inductive in nature. The inducer for ADH synthesis was not restricted to the substrate of the enzyme. Aspartic acid, threonine, both enantiomorphs of alanine and pyruvate were all effective.During the fractionation of the enzymes, GDH and the induced ADH were coexistent in the same fraction and distributed in almost the same proportions....

The glutamic dehydrogenase(GDH)-positive mutant, B. subtilis IRC-3-N-1, produced alanine dehydrogenase(ADH)when grown in nutrient medium. The formation of ADH by this mutant was inductive in nature. The inducer for ADH synthesis was not restricted to the substrate of the enzyme. Aspartic acid, threonine, both enantiomorphs of alanine and pyruvate were all effective.During the fractionation of the enzymes, GDH and the induced ADH were coexistent in the same fraction and distributed in almost the same proportions. The inductive ADH was found to be indistinguishable from its constitutive form in the wild type in regard to electrophoretic mobility. Since inductive formation of ADH did not cause a corresponding decrease of the constitutive GDH and contrarily, inhibition of ADH synthesis did not bring about any change of the level of the GDH in the mutant cells, the possibility that GDH was converted metabolitically to ADH was ruled out.All the GDH-positive mutants including those obtained by nitrous acid treatment and those of spontaneous origin are capable of inductive synthesis of ADH.In view of the inducibility of ADH synthesis in the GDH-positive mutants and the foregoing data on the immunology of these two enzymes, it was assumed that the genetic transition of ADH to GDH was a result of the mutation of a regulatory gene concerned in the synthesis of ADH.

枯草杆菌B.subtilis IRC-3-N-1,GDH~+变种在营养培养基上生长时能产生ADH。这样合成的ADH是誘导性貭的。誘导物除了酶的底物,L-丙氨酸外,尚有DL-天門冬氨酸,DL-苏氨酸,D-丙氨酸与丙酮酸。将酶进行純化时,GDH与誘导性的ADH始終同时存在于每一步驟中,而且两者的比例不变。誘导性的ADH与野生型细菌的本貭性ADH在电泳移动率上沒有区別。由于ADH誘导形成时,并末观察到变种細胞內GDH的相应減少,同时抑制了ADH的誘导合成,并不引起GDH水平的变化,因之由后者直接轉变成前者的可能性并不存在。所有GDH~+变种,包括自亚硝酸誘发突变或自发突变来源的,都能誘导生成ADH。从以上这些試驗結果,以及以前关于ADH与GDH免疫学試驗的結果,作者們认为枯草杆菌中,由亚硝酸誘发突变而引起ADH轉換成GDH的原因是由于与ADH合成有关的調节基因发生突变的結果。

In many important types of reactions involving olefins or acetylenes, catalyzed by compounds of the transition metals, activation of the unsaturated reacting groups is probably effected through the formation of δπ -bonds with the catalysts. In the present article, a critical review of the mechanisms of these important types of teactions is given. The relation between catalysis by coordination activation and the catalytic behaviours of transition metals, oxide semi-conductors and acid catalysts, and the trend...

In many important types of reactions involving olefins or acetylenes, catalyzed by compounds of the transition metals, activation of the unsaturated reacting groups is probably effected through the formation of δπ -bonds with the catalysts. In the present article, a critical review of the mechanisms of these important types of teactions is given. The relation between catalysis by coordination activation and the catalytic behaviours of transition metals, oxide semi-conductors and acid catalysts, and the trend of development in modern theory of catalysis are also briefly discussed.

在不饱和有机物所参与的许多类型的催化反应中,过渡金属化合物催化剂的作用可认为是通过与反应分子中的不饱和反应基因构成σπ-配键,从而使其活化的.本文根据这概念讨论了烯烃化学中某些重要的催化反应和催化剂的作用机理;最后并扼要地讨论了络合活化催化作用与金属催化剂、氧化物半导体催化剂和酸催化剂的催化性能的关系,以及催化理论的发展动向,

 
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