Objective To observe the effect of ketamine on Glutamate-induced apoptosis and the intracellular dissociated Ca~(2+) concentration([Ca~(2+)]i) in rat cultured spinal cord dorsal horn astrocytes.
Objective To observe the effect of different concentrations of propofol on glutamate- induced astrocyte injury in the dorsal horn of the spinal cord in rats.
Methods Using RT-PCR and Western Blot methods,the changes of AMPA receptor GluR2 subunit mRNA and protein expressions were observed in cultured hippocampal neurons of rats induced by glutamate.
Par-4-AS-ODN significantly suppress the up-regulation of intracellular calc ium concentration in PC12 cells induced by glutamate(Rate of fluorescent densit y: 167.9±32.4 vs 228.8±36.8, P<0.01).
Current data on glutamate-induced functional and morphological changes in mitochondria correlating with or being a result of their membrane potential changes are reviewed.
It is assumed that glutamate-induced loss of mitochondrial potential is mediated by Ca2+ overload resulting in the induction of nonspecific permeability of the inner mitochondrial membrane.
Objective:To investigate the effects of cholecystokinin-octapeptide(CCK-8)on glutamate-induced neurotoxicity in rat cerebral cortical neurons.Methods:The cytomorphological changes of cul-tured neuron treated by various factors were observed with scanning electron microscopy and LDH Iev-els in medium were measured with LDG-L kit.Results:CCK-8 may significantly attenuate destruction of neuronal structure induced by glutamate,and inhibit the LDH Ievels. The action of CCK-8 was blocked by CCK_B receptor antagon...
To study action of IL-1 involved in neurotoxicity. Methods: The effects of IL-1ra(IL-1 receptor antagonist, IL-1ra) on glutamate-induced neurotoxicity were examined using cultured ratcortical neurons with Trypan blue exclusion and scan electromicroscope. Meanwhile, effects of IL-1β onexpression of NMDAR1 mRNA were observed in cultures. Results: IL-1ra (1mg/L) may significantlyinhibit neuronal damage induced by glutamate at concentration of 0. 5 mmol/L F IL-1β dose-deppndentlypromoted expression of NMDAR1 mR...
IM: To determine whether the extract of leaves of Ginkgo biloba L (EGb) and several active constituents of EGb have protective effects against glutamate (Glu) induced neuronal damage. METHODS: Microscopy and image analysis of nucleus areas in the arcuate nuclei (AN) of mice were made. The neuronal viability in primary cultures from mouse cerebral cortex was assessed using MTT [3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium bromide] staining and the intracellular free calcium concentration ([...